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- W3100670310 abstract "Abstract Protein lipoylation is a post‐translational modification of emerging importance in both prokaryotes and eukaryotes. However, labeling and large‐scale profiling of protein lipoylation remain challenging. Here, we report the development of iLCL (iodoacetamide‐assisted lipoate‐cyclooctyne ligation), a chemoselective reaction that enables chemical tagging of protein lipoylation. We demonstrate that the cyclic disulfide of lipoamide but not linear disulfides can selectively react with iodoacetamide to produce sulfenic acid, which can be conjugated with cyclooctyne probes. iLCL enables tagging of lipoylated proteins for gel‐based detection and cellular imaging. Furthermore, we apply iLCL for proteomic profiling of lipoylated proteins in both bacteria and mammalian cells. In addition to all of the eight known lipoylated proteins, we identified seven candidates for novel lipoylated proteins. The iLCL strategy should facilitate uncovering the biological function of protein lipoylation." @default.
- W3100670310 created "2020-11-23" @default.
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- W3100670310 date "2020-12-21" @default.
- W3100670310 modified "2023-10-14" @default.
- W3100670310 title "Chemical Tagging of Protein Lipoylation" @default.
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- W3100670310 doi "https://doi.org/10.1002/anie.202010981" @default.
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