FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3100713060> ?p ?o ?g. }

• W3100713060 endingPage "8694" @default.
• W3100713060 startingPage "8694" @default.
• W3100713060 abstract "Defining the best combination of cells and biomaterials is a key challenge for the development of tendon tissue engineering (TE) strategies. Adipose-derived stem cells (ASCs) are ideal candidates for this purpose. In addition, controlled cell-based products adherent to good manufacturing practice (GMP) are required for their clinical scale-up. With this aim, in this study, ASC 3D bioprinting and GMP-compliant tenogenic differentiation were investigated. In detail, primary human ASCs were embedded within a nanofibrillar-cellulose/alginate bioink and 3D-bioprinted into multi-layered square-grid matrices. Bioink viscoelastic properties and scaffold ultrastructural morphology were analyzed by rheology and scanning electron microscopy (SEM). The optimal cell concentration for printing among 3, 6 and 9 × 106 ASC/mL was evaluated in terms of cell viability. ASC morphology was characterized by SEM and F-actin immunostaining. Tenogenic differentiation ability was then evaluated in terms of cell viability, morphology and expression of scleraxis and collagen type III by biochemical induction using BMP-12, TGF-β3, CTGF and ascorbic acid supplementation (TENO). Pro-inflammatory cytokine release was also assessed. Bioprinted ASCs showed high viability and survival and exhibited a tenocyte-like phenotype after biochemical induction, with no inflammatory response to the bioink. In conclusion, we report a first proof of concept for the clinical scale-up of ASC 3D bioprinting for tendon TE." @default.
• W3100713060 created "2020-11-23" @default.
• W3100713060 creator A5015303950 @default.
• W3100713060 creator A5036427354 @default.
• W3100713060 creator A5041672765 @default.
• W3100713060 creator A5043068469 @default.
• W3100713060 creator A5051663327 @default.
• W3100713060 creator A5062357921 @default.
• W3100713060 creator A5068491882 @default.
• W3100713060 date "2020-11-18" @default.
• W3100713060 modified "2023-10-14" @default.
• W3100713060 title "3D Bioprinting of Human Adipose-Derived Stem Cells and Their Tenogenic Differentiation in Clinical-Grade Medium" @default.
• W3100713060 cites W1482025997 @default.
• W3100713060 cites W1572018222 @default.
• W3100713060 cites W1965056469 @default.
• W3100713060 cites W1967415478 @default.
• W3100713060 cites W1971500643 @default.
• W3100713060 cites W1972610645 @default.
• W3100713060 cites W1972930867 @default.
• W3100713060 cites W1974163213 @default.
• W3100713060 cites W1982356500 @default.
• W3100713060 cites W1991931230 @default.
• W3100713060 cites W1994903148 @default.
• W3100713060 cites W1996113920 @default.
• W3100713060 cites W2001662737 @default.
• W3100713060 cites W2021128702 @default.
• W3100713060 cites W2033641981 @default.
• W3100713060 cites W2048644480 @default.
• W3100713060 cites W2054992092 @default.
• W3100713060 cites W2067812985 @default.
• W3100713060 cites W2071082100 @default.
• W3100713060 cites W2083994991 @default.
• W3100713060 cites W2084257603 @default.
• W3100713060 cites W2088278979 @default.
• W3100713060 cites W2107503723 @default.
• W3100713060 cites W2112957988 @default.
• W3100713060 cites W2115062578 @default.
• W3100713060 cites W2122822855 @default.
• W3100713060 cites W2133683725 @default.
• W3100713060 cites W2137571679 @default.
• W3100713060 cites W2138038251 @default.
• W3100713060 cites W2145062234 @default.
• W3100713060 cites W2153990758 @default.
• W3100713060 cites W2171162651 @default.
• W3100713060 cites W2171492615 @default.
• W3100713060 cites W2271618911 @default.
• W3100713060 cites W2290751882 @default.
• W3100713060 cites W2304916030 @default.
• W3100713060 cites W2328240320 @default.
• W3100713060 cites W2396518997 @default.
• W3100713060 cites W2482231493 @default.
• W3100713060 cites W2510240060 @default.
• W3100713060 cites W2520718776 @default.
• W3100713060 cites W2521121173 @default.
• W3100713060 cites W2585744746 @default.
• W3100713060 cites W2588746409 @default.
• W3100713060 cites W2593501280 @default.
• W3100713060 cites W2601097860 @default.
• W3100713060 cites W2604516108 @default.
• W3100713060 cites W2652296546 @default.
• W3100713060 cites W2738450069 @default.
• W3100713060 cites W2746198675 @default.
• W3100713060 cites W2755125191 @default.
• W3100713060 cites W2756672088 @default.
• W3100713060 cites W2759886011 @default.
• W3100713060 cites W2774582908 @default.
• W3100713060 cites W2785325647 @default.
• W3100713060 cites W2793358356 @default.
• W3100713060 cites W2888737314 @default.
• W3100713060 cites W2909360950 @default.
• W3100713060 cites W2911376101 @default.
• W3100713060 cites W2912026146 @default.
• W3100713060 cites W2937732269 @default.
• W3100713060 cites W2980195275 @default.
• W3100713060 cites W2981931524 @default.
• W3100713060 cites W3030898095 @default.
• W3100713060 cites W4211147424 @default.
• W3100713060 cites W4255895078 @default.
• W3100713060 doi "https://doi.org/10.3390/ijms21228694" @default.
• W3100713060 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7698777" @default.
• W3100713060 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33218011" @default.
• W3100713060 hasPublicationYear "2020" @default.
• W3100713060 type Work @default.
• W3100713060 sameAs 3100713060 @default.
• W3100713060 citedByCount "17" @default.
• W3100713060 countsByYear W31007130602021 @default.
• W3100713060 countsByYear W31007130602022 @default.
• W3100713060 countsByYear W31007130602023 @default.
• W3100713060 crossrefType "journal-article" @default.
• W3100713060 hasAuthorship W3100713060A5015303950 @default.
• W3100713060 hasAuthorship W3100713060A5036427354 @default.
• W3100713060 hasAuthorship W3100713060A5041672765 @default.
• W3100713060 hasAuthorship W3100713060A5043068469 @default.
• W3100713060 hasAuthorship W3100713060A5051663327 @default.
• W3100713060 hasAuthorship W3100713060A5062357921 @default.
• W3100713060 hasAuthorship W3100713060A5068491882 @default.
• W3100713060 hasBestOaLocation W31007130601 @default.
• W3100713060 hasConcept C122927707 @default.

• next