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- W3100764059 abstract "Abstract Metritis is an important disorder in dairy cows during the early postpartum period. Myometrial contractility is a prerequisite for uterine involution; however, very scanty literature is available about the effect of metritis on this process and endocrine responsiveness. This study was aimed to evaluate the effect of inflammation on uterine contractility in vitro, and the inflammation was induced by incubating myometrial strips with lipopolysaccharides (LPS). Myometrial samples were collected from 17 healthy Holstein Friesian cows during caesarean section. Eight longitudinal strips from each cow were incubated in organ baths with LPS concentrations of 0 (LPS 0 ), 0.1 (LPS 0.1 ), 1 (LPS 1 ) and 10 µg/ml (LPS 10 ). Spontaneous contractility and contractility induced by increasing concentrations of oxytocin (10 –10 – 10 –7 mol/L) were recorded during nine 30‐min intervals (T1 to T9). The minimum amplitude (minA), maximum amplitude (maxA), mean amplitude (meanA) and area under the curve (AUC) were calculated for each time interval. LPS had an effect ( p ≤ .05) on maxA, meanA and AUC. In T1, myometrial strips incubated with LPS 0.1 and LPS 1 had higher ( p ≤ .05) maxA, meanA and AUC than the strips incubated with LPS 0 . In T9 without oxytocin, LPS 0 led to higher ( p ≤ .05) maxA, meanA and AUC than LPS 0.1 and LPS 1 . In T8 and T9 with oxytocin, LPS 1 had lower ( p ≤ .05) maxA, meanA and AUC than the other LPS concentrations. Interestingly, the results show that LPS has a transient positive effect on myometrial contractility in vitro and that this effect is dependent on LPS concentration and duration of incubation." @default.
- W3100764059 created "2020-11-23" @default.
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- W3100764059 date "2020-12-01" @default.
- W3100764059 modified "2023-10-18" @default.
- W3100764059 title "In vitro effects of lipopolysaccharides on bovine uterine contractility" @default.
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- W3100764059 doi "https://doi.org/10.1111/rda.13862" @default.
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