FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3100796800> ?p ?o ?g. }

• W3100796800 abstract "Flow cytometry represents a powerful and evolving methodologic approach to cell adhesion biology. In its beginnings, flow cytometry was used solely to measure the expression of receptors on cellular surfaces and to correlate that expression with biologic function in non-flow-cytometry-based assays. From this primitive beginning, applications have proliferated and now include methodologies that measure real-time aggregation, receptor activity, and the downstream biologic consequences of cell adhesion. These biologic applications have led to platforms that are easily employed as drug screening and target validation tools. Functional assays that measure cell aggregation were initially developed to measure cell–cell interactions in the immune system, especially between cytotoxic cells and various cell types targeted as the focus of their cytotoxic activity. The cytotoxic “effector” cells and the “target” cells were stained with spectrally distinct fluorescent dyes, gently sedimented together into a cell pellet, and allowed to interact under static conditions for designated intervals of time. When resuspended and introduced into the flow cytometer, effector cells adherent to target cells were detected as “conjugate” particles emitting the fluorescence spectra of both dyes. Nonadherent effector and target cells were detected as monochromatically fluorescent particles. By using ion concentration–sensitive cytoplasmic fluorescent probes as the effector cell labels, it was also possible to detect physiological changes in intracellular ionized calcium and pH elicited by adhesion to target cells and to correlate these responses with cytotoxic function. Later, methods were developed for continuously measuring (“real-time”) cell adhesive interactions as they progressed over time in a fluid shear environment. A limitation of early adhesion kinetics analyses was that the fluid shear was generated with a magnetic stir bar and was thus neither homogeneous nor amenable to precise quantification. Subsequent refinement of these methods has enabled flow cytometric analysis of cell mixtures subjected to a more uniform and quantifiable fluid shear environment generated in a cone-plate viscometer. Cell mixtures are sampled periodically from the viscometer into a formalin fixative solution for subsequent off-line flow cytometric analysis. These experiments have been able to demonstrate a remarkable potentiation of adhesion efficiency through the combined action of two sets of adhesion molecules and a progression of adhesion molecule use from one class to another over time." @default.
• W3100796800 created "2020-11-23" @default.
• W3100796800 creator A5071867144 @default.
• W3100796800 creator A5075766799 @default.
• W3100796800 date "2005-09-22" @default.
• W3100796800 modified "2023-09-27" @default.
• W3100796800 title "Applications of Flow Cytometry to Cell Adhesion Biology: From Aggregates to Drug Discovery" @default.
• W3100796800 doi "https://doi.org/10.1093/oso/9780195183146.003.0023" @default.
• W3100796800 hasPublicationYear "2005" @default.
• W3100796800 type Work @default.
• W3100796800 sameAs 3100796800 @default.
• W3100796800 citedByCount "0" @default.
• W3100796800 crossrefType "book-chapter" @default.
• W3100796800 hasAuthorship W3100796800A5071867144 @default.
• W3100796800 hasAuthorship W3100796800A5075766799 @default.
• W3100796800 hasConcept C12554922 @default.
• W3100796800 hasConcept C1491633281 @default.
• W3100796800 hasConcept C153911025 @default.
• W3100796800 hasConcept C154317977 @default.
• W3100796800 hasConcept C178790620 @default.
• W3100796800 hasConcept C185592680 @default.
• W3100796800 hasConcept C190062978 @default.
• W3100796800 hasConcept C202751555 @default.
• W3100796800 hasConcept C2780339063 @default.
• W3100796800 hasConcept C51785407 @default.
• W3100796800 hasConcept C553184892 @default.
• W3100796800 hasConcept C55493867 @default.
• W3100796800 hasConcept C79879829 @default.
• W3100796800 hasConcept C84416704 @default.
• W3100796800 hasConcept C85789140 @default.
• W3100796800 hasConcept C86803240 @default.
• W3100796800 hasConcept C95444343 @default.
• W3100796800 hasConceptScore W3100796800C12554922 @default.
• W3100796800 hasConceptScore W3100796800C1491633281 @default.
• W3100796800 hasConceptScore W3100796800C153911025 @default.
• W3100796800 hasConceptScore W3100796800C154317977 @default.
• W3100796800 hasConceptScore W3100796800C178790620 @default.
• W3100796800 hasConceptScore W3100796800C185592680 @default.
• W3100796800 hasConceptScore W3100796800C190062978 @default.
• W3100796800 hasConceptScore W3100796800C202751555 @default.
• W3100796800 hasConceptScore W3100796800C2780339063 @default.
• W3100796800 hasConceptScore W3100796800C51785407 @default.
• W3100796800 hasConceptScore W3100796800C553184892 @default.
• W3100796800 hasConceptScore W3100796800C55493867 @default.
• W3100796800 hasConceptScore W3100796800C79879829 @default.
• W3100796800 hasConceptScore W3100796800C84416704 @default.
• W3100796800 hasConceptScore W3100796800C85789140 @default.
• W3100796800 hasConceptScore W3100796800C86803240 @default.
• W3100796800 hasConceptScore W3100796800C95444343 @default.
• W3100796800 hasLocation W31007968001 @default.
• W3100796800 hasOpenAccess W3100796800 @default.
• W3100796800 hasPrimaryLocation W31007968001 @default.
• W3100796800 hasRelatedWork W2001837649 @default.
• W3100796800 hasRelatedWork W2023279715 @default.
• W3100796800 hasRelatedWork W2036353667 @default.
• W3100796800 hasRelatedWork W2058854575 @default.
• W3100796800 hasRelatedWork W2079245482 @default.
• W3100796800 hasRelatedWork W2080540673 @default.
• W3100796800 hasRelatedWork W2584128045 @default.
• W3100796800 hasRelatedWork W2755915064 @default.
• W3100796800 hasRelatedWork W3012197598 @default.
• W3100796800 hasRelatedWork W3016124788 @default.
• W3100796800 isParatext "false" @default.
• W3100796800 isRetracted "false" @default.
• W3100796800 magId "3100796800" @default.
• W3100796800 workType "book-chapter" @default.