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- W3100826046 abstract "Ribosomes often encounter obstacles during translation elongation and thus collide with each other. Disome profiling, an optimized ribosome profiling method, specifically sequences the long ribosome footprints generated from collided ribosomes produced by the ribosome pause and thus allows the survey of sites in a genome-wide manner. This protocol details the procedure from lysate preparation of human tissue cultures and zebrafish embryos to sequencing library construction. For complete details on the use and execution of this protocol, please refer to Han et al. (2020)." @default.
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- W3100826046 date "2020-12-01" @default.
- W3100826046 modified "2023-10-16" @default.
- W3100826046 title "Protocol for Disome Profiling to Survey Ribosome Collision in Humans and Zebrafish" @default.
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- W3100826046 doi "https://doi.org/10.1016/j.xpro.2020.100168" @default.
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