FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3100939609> ?p ?o ?g. }

• W3100939609 endingPage "3001" @default.
• W3100939609 startingPage "2991" @default.
• W3100939609 abstract "The aim of this study was to investigate the role of miRNA-486-5p in chronic obstructive pulmonary disease (COPD) progression and the underlying molecular mechanisms.Aberrant miRNA expression profiles between smokers and nonsmokers, and those between COPD patients and normal subjects were analyzed using microarray datasets and reverse-transcriptase quantitative polymerase chain reaction (qPCR). Enzyme-linked immunosorbent assay was used to determine the levels of inflammatory cytokines in cell supernatants. Expression levels of inflammatory cytokines, HAT1, TLR4, and miR-486-5p, were determined using qPCR or Western blotting. Luciferase reporter assays and fluorescence in situ hybridization were used to confirm the regulatory interaction between miR-486-6p and HAT1.miR-486-5p was significantly upregulated in the COPD and smoker groups compared to the control group, as demonstrated using bioinformatics analysis and validated using qPCR assay of alveolar macrophages and peripheral monocytes. Moreover, miR-486-5p expression was significantly correlated with the expression of IL-6, IL-8, TNF-α, and IFN-γ. Luciferase reporter assays confirmed that miR-486-5p directly targeted HAT1, and cellular localization showed that miR-486-5p and HAT1 were highly expressed in the cytoplasm. miR-486-5p overexpression led to a significant upregulation of TLR4 and a significant downregulation of HAT1. Inversely, miR-486-5p inhibition led to a significant downregulation of TLR4 and a significant upregulation of HAT1. HAT1 knockdown using siRNA significantly upregulated the expression of TLR4, IL-6, IL-8, TNF-α, and IFN-γ.miR-486-5p was differentially expressed in the alveolar macrophages of COPD patients. miR-486-5p overexpression may enhance the TLR4-triggered inflammatory response in COPD patients by targeting HAT1." @default.
• W3100939609 created "2020-11-23" @default.
• W3100939609 creator A5010379353 @default.
• W3100939609 creator A5054267674 @default.
• W3100939609 creator A5064968552 @default.
• W3100939609 creator A5071127825 @default.
• W3100939609 creator A5077715966 @default.
• W3100939609 creator A5080847607 @default.
• W3100939609 creator A5091285946 @default.
• W3100939609 date "2020-11-01" @default.
• W3100939609 modified "2023-09-27" @default.
• W3100939609 title "<p>miRNA-486-5p Promotes COPD Progression by Targeting HAT1 to Regulate the TLR4-Triggered Inflammatory Response of Alveolar Macrophages</p>" @default.
• W3100939609 cites W1705531132 @default.
• W3100939609 cites W1982263285 @default.
• W3100939609 cites W2004927458 @default.
• W3100939609 cites W2012593128 @default.
• W3100939609 cites W2053429123 @default.
• W3100939609 cites W2081720358 @default.
• W3100939609 cites W2091426331 @default.
• W3100939609 cites W2109491705 @default.
• W3100939609 cites W2111374619 @default.
• W3100939609 cites W2126420549 @default.
• W3100939609 cites W2133875700 @default.
• W3100939609 cites W2134629862 @default.
• W3100939609 cites W2137734450 @default.
• W3100939609 cites W2186695811 @default.
• W3100939609 cites W2198015960 @default.
• W3100939609 cites W2211837538 @default.
• W3100939609 cites W2316119938 @default.
• W3100939609 cites W2401306489 @default.
• W3100939609 cites W2474216046 @default.
• W3100939609 cites W2513389639 @default.
• W3100939609 cites W2530460077 @default.
• W3100939609 cites W2584465173 @default.
• W3100939609 cites W2590701429 @default.
• W3100939609 cites W2604137520 @default.
• W3100939609 cites W2610231217 @default.
• W3100939609 cites W2619076532 @default.
• W3100939609 cites W2740775631 @default.
• W3100939609 cites W2750383441 @default.
• W3100939609 cites W2753490904 @default.
• W3100939609 cites W2760493428 @default.
• W3100939609 cites W2766280052 @default.
• W3100939609 cites W2767546566 @default.
• W3100939609 cites W2791014718 @default.
• W3100939609 cites W2878649895 @default.
• W3100939609 cites W2887367079 @default.
• W3100939609 cites W2892139376 @default.
• W3100939609 cites W2894766323 @default.
• W3100939609 cites W2903685938 @default.
• W3100939609 cites W2904083228 @default.
• W3100939609 cites W2979744254 @default.
• W3100939609 cites W2995898781 @default.
• W3100939609 cites W3187803651 @default.
• W3100939609 doi "https://doi.org/10.2147/copd.s280614" @default.
• W3100939609 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7683830" @default.
• W3100939609 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33244226" @default.
• W3100939609 hasPublicationYear "2020" @default.
• W3100939609 type Work @default.
• W3100939609 sameAs 3100939609 @default.
• W3100939609 citedByCount "15" @default.
• W3100939609 countsByYear W31009396092021 @default.
• W3100939609 countsByYear W31009396092022 @default.
• W3100939609 countsByYear W31009396092023 @default.
• W3100939609 crossrefType "journal-article" @default.
• W3100939609 hasAuthorship W3100939609A5010379353 @default.
• W3100939609 hasAuthorship W3100939609A5054267674 @default.
• W3100939609 hasAuthorship W3100939609A5064968552 @default.
• W3100939609 hasAuthorship W3100939609A5071127825 @default.
• W3100939609 hasAuthorship W3100939609A5077715966 @default.
• W3100939609 hasAuthorship W3100939609A5080847607 @default.
• W3100939609 hasAuthorship W3100939609A5091285946 @default.
• W3100939609 hasBestOaLocation W31009396091 @default.
• W3100939609 hasConcept C104317684 @default.
• W3100939609 hasConcept C127561419 @default.
• W3100939609 hasConcept C145059251 @default.
• W3100939609 hasConcept C150194340 @default.
• W3100939609 hasConcept C153911025 @default.
• W3100939609 hasConcept C164027704 @default.
• W3100939609 hasConcept C173396325 @default.
• W3100939609 hasConcept C17991360 @default.
• W3100939609 hasConcept C190283241 @default.
• W3100939609 hasConcept C203014093 @default.
• W3100939609 hasConcept C2776070231 @default.
• W3100939609 hasConcept C2776914184 @default.
• W3100939609 hasConcept C48023723 @default.
• W3100939609 hasConcept C502942594 @default.
• W3100939609 hasConcept C55493867 @default.
• W3100939609 hasConcept C71924100 @default.
• W3100939609 hasConcept C8415881 @default.
• W3100939609 hasConcept C86803240 @default.
• W3100939609 hasConceptScore W3100939609C104317684 @default.
• W3100939609 hasConceptScore W3100939609C127561419 @default.
• W3100939609 hasConceptScore W3100939609C145059251 @default.
• W3100939609 hasConceptScore W3100939609C150194340 @default.
• W3100939609 hasConceptScore W3100939609C153911025 @default.
• W3100939609 hasConceptScore W3100939609C164027704 @default.
• W3100939609 hasConceptScore W3100939609C173396325 @default.

• next