Matches in SemOpenAlex for { <https://semopenalex.org/work/W3100958907> ?p ?o ?g. }
- W3100958907 abstract "Abstract Crosstalk between malignant and neighboring cells contributes to tumor growth. In East Asia, infection with fish-borne liver flukes is a major risk factor for cholangiocarcinoma (CCA). The parasite secretes a growth factor termed liver fluke granulin ( Ov -GRN-1), a homologue of the human progranulin (huPGRN), which contributes significantly to biliary tract fibrosis and morbidity during infection. Here, exosome-mediated transfer of mRNAs from the human cholangiocyte cell line (H69) was investigated following exposure to Ov- GRN-1, to naïve recipient H69 cells. To minimize the influence of endogenous huPGRN, the gene encoding huPGRN was inactivated using CRISPR/Cas9-based gene knock-out. Several huPGRN-depleted cell lines, termed ΔhuPGRN-H69, were established. These lines exhibited >80% reductions in levels of huPGRN transcripts and protein, both in gene-edited cells and within exosomes released by these cells. Profiles of exosomal RNAs (exRNA) from ΔhuPGRN-H69 for CCA-associated characteristics revealed a paucity of transcripts for estrogen- and Wnt-signaling pathways, peptidase inhibitors and tyrosine phosphatase related to cellular processes including oncogenic transformation. Several CCA-specific mRNAs including MAPK/AKT pathway members were induced by exposure to Ov -GRN-1. By comparison, estrogen, Wnt/PI3K and TGF signaling and other CCA pathway mRNAs were upregulated in wild type H69 exposed to Ov -GRN-1. Of these, CCA-associated exRNAs modified the CCA microenvironment in naïve recipient cells co-cultured with exosomes from ΔhuPGRN-H69 exposed to Ov- GRN-1, and induced translation of MAPK phosphorylation related-protein in naïve recipient cells comparing with control recipient cells. Exosome-mediated crosstalk in response to liver fluke granulin promoted a CCA-specific program through MAPK pathway which, in turn, established a CCA-conducive disposition." @default.
- W3100958907 created "2020-11-23" @default.
- W3100958907 creator A5001125098 @default.
- W3100958907 creator A5004216416 @default.
- W3100958907 creator A5007882171 @default.
- W3100958907 creator A5015076708 @default.
- W3100958907 creator A5025133816 @default.
- W3100958907 creator A5028059814 @default.
- W3100958907 creator A5035941607 @default.
- W3100958907 creator A5041928780 @default.
- W3100958907 creator A5055394762 @default.
- W3100958907 creator A5057395686 @default.
- W3100958907 creator A5071377385 @default.
- W3100958907 creator A5071410567 @default.
- W3100958907 creator A5074950470 @default.
- W3100958907 creator A5087460086 @default.
- W3100958907 date "2019-07-14" @default.
- W3100958907 modified "2023-09-27" @default.
- W3100958907 title "Liver fluke granulin promotes exosome-mediated crosstalk and cellular microenvironment conducive to cholangiocarcinoma" @default.
- W3100958907 cites W1881130638 @default.
- W3100958907 cites W1964488322 @default.
- W3100958907 cites W1968427508 @default.
- W3100958907 cites W1974564771 @default.
- W3100958907 cites W1975563887 @default.
- W3100958907 cites W1976086500 @default.
- W3100958907 cites W1984878005 @default.
- W3100958907 cites W1990350409 @default.
- W3100958907 cites W1990484563 @default.
- W3100958907 cites W1998540184 @default.
- W3100958907 cites W2003459911 @default.
- W3100958907 cites W2010790600 @default.
- W3100958907 cites W2018369483 @default.
- W3100958907 cites W2023471806 @default.
- W3100958907 cites W2038944440 @default.
- W3100958907 cites W2051432136 @default.
- W3100958907 cites W2068427957 @default.
- W3100958907 cites W2071765960 @default.
- W3100958907 cites W2075953048 @default.
- W3100958907 cites W2083296923 @default.
- W3100958907 cites W2094395605 @default.
- W3100958907 cites W2097023342 @default.
- W3100958907 cites W2097761110 @default.
- W3100958907 cites W2107277218 @default.
- W3100958907 cites W2111775782 @default.
- W3100958907 cites W2115892230 @default.
- W3100958907 cites W2120418776 @default.
- W3100958907 cites W2122675979 @default.
- W3100958907 cites W2125508818 @default.
- W3100958907 cites W2129692155 @default.
- W3100958907 cites W2140030329 @default.
- W3100958907 cites W2141042450 @default.
- W3100958907 cites W2154156417 @default.
- W3100958907 cites W2162021059 @default.
- W3100958907 cites W2260292996 @default.
- W3100958907 cites W2267404846 @default.
- W3100958907 cites W2470801200 @default.
- W3100958907 cites W2485316353 @default.
- W3100958907 cites W2519687050 @default.
- W3100958907 cites W2528619219 @default.
- W3100958907 cites W2558669106 @default.
- W3100958907 cites W2559505560 @default.
- W3100958907 cites W2606520907 @default.
- W3100958907 cites W2620210866 @default.
- W3100958907 cites W2724543993 @default.
- W3100958907 cites W2741941234 @default.
- W3100958907 cites W2761560873 @default.
- W3100958907 cites W2782752196 @default.
- W3100958907 cites W2786514708 @default.
- W3100958907 cites W2791527635 @default.
- W3100958907 cites W2792541019 @default.
- W3100958907 cites W2797336915 @default.
- W3100958907 cites W2811415666 @default.
- W3100958907 cites W2889607525 @default.
- W3100958907 cites W2892899495 @default.
- W3100958907 cites W2895255040 @default.
- W3100958907 cites W2899807648 @default.
- W3100958907 cites W2900482673 @default.
- W3100958907 cites W2900735818 @default.
- W3100958907 cites W2901440033 @default.
- W3100958907 cites W2910099164 @default.
- W3100958907 cites W2920849174 @default.
- W3100958907 cites W2943740964 @default.
- W3100958907 cites W2950320278 @default.
- W3100958907 cites W2953363910 @default.
- W3100958907 cites W4238000034 @default.
- W3100958907 cites W4293247451 @default.
- W3100958907 cites W96287323 @default.
- W3100958907 doi "https://doi.org/10.1101/700427" @default.
- W3100958907 hasPublicationYear "2019" @default.
- W3100958907 type Work @default.
- W3100958907 sameAs 3100958907 @default.
- W3100958907 citedByCount "0" @default.
- W3100958907 crossrefType "posted-content" @default.
- W3100958907 hasAuthorship W3100958907A5001125098 @default.
- W3100958907 hasAuthorship W3100958907A5004216416 @default.
- W3100958907 hasAuthorship W3100958907A5007882171 @default.
- W3100958907 hasAuthorship W3100958907A5015076708 @default.
- W3100958907 hasAuthorship W3100958907A5025133816 @default.
- W3100958907 hasAuthorship W3100958907A5028059814 @default.
- W3100958907 hasAuthorship W3100958907A5035941607 @default.