FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3100991662> ?p ?o ?g. }

• W3100991662 abstract "Abstract The mammalian brain consists of several structurally and functionally distinct regions equipped with an equally complex cell-type system. Due to its relevance in uncovering disease mechanisms, the study of cell-type-specific molecular signatures of different brain regions has increased. The rapid evolution of newer and cheaper sequencing techniques has also boosted the interest in cell-type-specific epigenetic studies. In fact, the nucleus holds most of the cell’s epigenetic information and is quite resistant to tissue dissociation processes as compared to cells. As such, nuclei are continually preferred over cells for epigenetic studies. However, the isolation of nuclei from cells is still a biochemically complex process, with every step affecting downstream results. Therefore, it is necessary to use protocols that fit the experimental design to yield nuclei of high quality and quantity. However, the current protocols are not suitable for nuclei isolation of small volumes of micro-dissected brain regions from individual mouse brains. Additionally, the caveats associated with centrifugation steps of nuclei extraction and the effects of different buffers have not been thoroughly investigated. Therefore, in this study, we describe an iodixanol based density gradient ultracentrifugation protocol suitable for micro-dissected brain regions from individual mice using Arc creERT2 (TG/WT) . R 26 CAG-Sun1-sfGFP-Myc (M/WT or M/M) . This mouse model shows sfGFP expression (sfGFP + ) in the nuclear membrane of specific stimulus activated cells, thereby providing a good basis for the study - nuclei isolation and separation of cell-type-specific nuclei. The study also introduces new tools for rapid visualization and assessment of quality and quantity of nascent extracted nuclei. These tools were then used to examine critical morphological features of nuclei derived from different centrifugation methods and the use of different buffers to uncover underlying effects. Finally, to obtain cell-type-specific nuclei (sfGFP + nuclei) from the isolated nuclei pool of high viscosity, an optimized protocol for fluorescence activated nuclei sorting (FANS) was established to speed up sorting. Additionally, we present a 1% PFA protocol for fixation of isolated nuclei for long term microscopic visualization." @default.
• W3100991662 created "2020-11-23" @default.
• W3100991662 creator A5002178404 @default.
• W3100991662 creator A5006364288 @default.
• W3100991662 creator A5010773811 @default.
• W3100991662 creator A5041984103 @default.
• W3100991662 creator A5053322122 @default.
• W3100991662 date "2020-11-18" @default.
• W3100991662 modified "2023-10-13" @default.
• W3100991662 title "Isolation of nuclei and downstream processing of cell-type-specific nuclei from micro-dissected mouse brain regions – techniques and caveats" @default.
• W3100991662 cites W1503169097 @default.
• W3100991662 cites W1570600309 @default.
• W3100991662 cites W16656028 @default.
• W3100991662 cites W1706884702 @default.
• W3100991662 cites W1935339094 @default.
• W3100991662 cites W1972218846 @default.
• W3100991662 cites W1981654563 @default.
• W3100991662 cites W2001849319 @default.
• W3100991662 cites W2005063559 @default.
• W3100991662 cites W2007060069 @default.
• W3100991662 cites W2019287022 @default.
• W3100991662 cites W2059435371 @default.
• W3100991662 cites W2074335624 @default.
• W3100991662 cites W2075476418 @default.
• W3100991662 cites W2078236387 @default.
• W3100991662 cites W2114664783 @default.
• W3100991662 cites W2154558473 @default.
• W3100991662 cites W2167497075 @default.
• W3100991662 cites W2185425783 @default.
• W3100991662 cites W2279350325 @default.
• W3100991662 cites W2406354252 @default.
• W3100991662 cites W2414083225 @default.
• W3100991662 cites W2766798615 @default.
• W3100991662 cites W2791004613 @default.
• W3100991662 cites W2883504180 @default.
• W3100991662 cites W2912652602 @default.
• W3100991662 cites W2972344703 @default.
• W3100991662 cites W2996352091 @default.
• W3100991662 doi "https://doi.org/10.1101/2020.11.18.374223" @default.
• W3100991662 hasPublicationYear "2020" @default.
• W3100991662 type Work @default.
• W3100991662 sameAs 3100991662 @default.
• W3100991662 citedByCount "4" @default.
• W3100991662 countsByYear W31009916622021 @default.
• W3100991662 countsByYear W31009916622022 @default.
• W3100991662 crossrefType "posted-content" @default.
• W3100991662 hasAuthorship W3100991662A5002178404 @default.
• W3100991662 hasAuthorship W3100991662A5006364288 @default.
• W3100991662 hasAuthorship W3100991662A5010773811 @default.
• W3100991662 hasAuthorship W3100991662A5041984103 @default.
• W3100991662 hasAuthorship W3100991662A5053322122 @default.
• W3100991662 hasBestOaLocation W31009916621 @default.
• W3100991662 hasConcept C104317684 @default.
• W3100991662 hasConcept C1491633281 @default.
• W3100991662 hasConcept C185592680 @default.
• W3100991662 hasConcept C189014844 @default.
• W3100991662 hasConcept C2780723820 @default.
• W3100991662 hasConcept C41091548 @default.
• W3100991662 hasConcept C54355233 @default.
• W3100991662 hasConcept C70721500 @default.
• W3100991662 hasConcept C86803240 @default.
• W3100991662 hasConcept C95444343 @default.
• W3100991662 hasConceptScore W3100991662C104317684 @default.
• W3100991662 hasConceptScore W3100991662C1491633281 @default.
• W3100991662 hasConceptScore W3100991662C185592680 @default.
• W3100991662 hasConceptScore W3100991662C189014844 @default.
• W3100991662 hasConceptScore W3100991662C2780723820 @default.
• W3100991662 hasConceptScore W3100991662C41091548 @default.
• W3100991662 hasConceptScore W3100991662C54355233 @default.
• W3100991662 hasConceptScore W3100991662C70721500 @default.
• W3100991662 hasConceptScore W3100991662C86803240 @default.
• W3100991662 hasConceptScore W3100991662C95444343 @default.
• W3100991662 hasLocation W31009916621 @default.
• W3100991662 hasOpenAccess W3100991662 @default.
• W3100991662 hasPrimaryLocation W31009916621 @default.
• W3100991662 hasRelatedWork W1965454525 @default.
• W3100991662 hasRelatedWork W2094214551 @default.
• W3100991662 hasRelatedWork W2222447005 @default.
• W3100991662 hasRelatedWork W2545003596 @default.
• W3100991662 hasRelatedWork W2795381779 @default.
• W3100991662 hasRelatedWork W2951914955 @default.
• W3100991662 hasRelatedWork W3004427646 @default.
• W3100991662 hasRelatedWork W3034061995 @default.
• W3100991662 hasRelatedWork W4296703446 @default.
• W3100991662 hasRelatedWork W4307358436 @default.
• W3100991662 isParatext "false" @default.
• W3100991662 isRetracted "false" @default.
• W3100991662 magId "3100991662" @default.
• W3100991662 workType "article" @default.