Matches in SemOpenAlex for { <https://semopenalex.org/work/W3101187332> ?p ?o ?g. }
- W3101187332 endingPage "121874" @default.
- W3101187332 startingPage "121874" @default.
- W3101187332 abstract "Herein, we developed a flexible and cost-effective manual droplet operation system (MDOS) for performing miniaturized cell assays as well as single cell analysis. The MDOS consists of a manual x-y-z translation stage for liquid transferring and switching, a high-precision syringe pump for liquid driving and metering, a tapered capillary probe for droplet manipulation, a droplet array chip for droplet loading and reaction, sample/reagent reservoirs for storage, and a microscope for droplet observation, with a total expense of only $4,000. By using the flexible combination of three elementary operations of the x-y-z stage's moving and the pump's aspirating and depositing, the MDOS can manually achieve multiple droplet handling operations in the nanoliter to picoliter range, including droplet generation, assembling, fusion, diluting, and splitting. On this basis, multiple cell-related operations could be performed, such as nanoliter-scale in-droplet cell culture, cell coculture, drug stimulation, cell washing, and cell staining, as well as formation of picoliter single-cell droplets. The feasibility and flexibility of the MDOS was demonstrated in multi-mode miniaturized cell assays, including cell-based drug test, first-pass effect assay, and single-cell enzyme assay. The MDOS with the features of low cost, easy to build and flexible to use, could provide a promising alternative for performing miniaturized assays in routine laboratories, in addition to conventional microfluidic chip-based systems and automated robot systems." @default.
- W3101187332 created "2020-11-23" @default.
- W3101187332 creator A5014288270 @default.
- W3101187332 creator A5040746847 @default.
- W3101187332 creator A5051941824 @default.
- W3101187332 creator A5077858309 @default.
- W3101187332 creator A5084617416 @default.
- W3101187332 date "2021-03-01" @default.
- W3101187332 modified "2023-10-15" @default.
- W3101187332 title "A flexible and cost-effective manual droplet operation platform for miniaturized cell assays and single cell analysis" @default.
- W3101187332 cites W1969894206 @default.
- W3101187332 cites W1971182486 @default.
- W3101187332 cites W1971500740 @default.
- W3101187332 cites W1975581000 @default.
- W3101187332 cites W1982964683 @default.
- W3101187332 cites W1984327317 @default.
- W3101187332 cites W1987362398 @default.
- W3101187332 cites W1992712806 @default.
- W3101187332 cites W1994504773 @default.
- W3101187332 cites W1994746525 @default.
- W3101187332 cites W1997088089 @default.
- W3101187332 cites W2001607419 @default.
- W3101187332 cites W2006759882 @default.
- W3101187332 cites W2013240219 @default.
- W3101187332 cites W2015554529 @default.
- W3101187332 cites W2016915024 @default.
- W3101187332 cites W2020438216 @default.
- W3101187332 cites W2024030519 @default.
- W3101187332 cites W2026908430 @default.
- W3101187332 cites W2028333733 @default.
- W3101187332 cites W2029162014 @default.
- W3101187332 cites W2045551515 @default.
- W3101187332 cites W2052715775 @default.
- W3101187332 cites W2057800506 @default.
- W3101187332 cites W2059018615 @default.
- W3101187332 cites W2062780817 @default.
- W3101187332 cites W2074640863 @default.
- W3101187332 cites W2075387868 @default.
- W3101187332 cites W2076922024 @default.
- W3101187332 cites W2088212087 @default.
- W3101187332 cites W2113214121 @default.
- W3101187332 cites W2124338782 @default.
- W3101187332 cites W2133811295 @default.
- W3101187332 cites W2134745814 @default.
- W3101187332 cites W2141789961 @default.
- W3101187332 cites W2163232083 @default.
- W3101187332 cites W2322862476 @default.
- W3101187332 cites W2328252368 @default.
- W3101187332 cites W2331001184 @default.
- W3101187332 cites W2334966290 @default.
- W3101187332 cites W2533636589 @default.
- W3101187332 cites W2557805030 @default.
- W3101187332 cites W2751974637 @default.
- W3101187332 cites W2786100953 @default.
- W3101187332 cites W2789614331 @default.
- W3101187332 cites W2790054076 @default.
- W3101187332 cites W2794526265 @default.
- W3101187332 cites W2798204822 @default.
- W3101187332 cites W2801354110 @default.
- W3101187332 cites W2886103315 @default.
- W3101187332 cites W2889308220 @default.
- W3101187332 cites W2907304027 @default.
- W3101187332 cites W2998738108 @default.
- W3101187332 cites W3006435583 @default.
- W3101187332 cites W3010423416 @default.
- W3101187332 cites W3014856783 @default.
- W3101187332 doi "https://doi.org/10.1016/j.talanta.2020.121874" @default.
- W3101187332 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33379083" @default.
- W3101187332 hasPublicationYear "2021" @default.
- W3101187332 type Work @default.
- W3101187332 sameAs 3101187332 @default.
- W3101187332 citedByCount "2" @default.
- W3101187332 countsByYear W31011873322022 @default.
- W3101187332 countsByYear W31011873322023 @default.
- W3101187332 crossrefType "journal-article" @default.
- W3101187332 hasAuthorship W3101187332A5014288270 @default.
- W3101187332 hasAuthorship W3101187332A5040746847 @default.
- W3101187332 hasAuthorship W3101187332A5051941824 @default.
- W3101187332 hasAuthorship W3101187332A5077858309 @default.
- W3101187332 hasAuthorship W3101187332A5084617416 @default.
- W3101187332 hasConcept C113196181 @default.
- W3101187332 hasConcept C118552586 @default.
- W3101187332 hasConcept C136229726 @default.
- W3101187332 hasConcept C147789679 @default.
- W3101187332 hasConcept C1491633281 @default.
- W3101187332 hasConcept C15744967 @default.
- W3101187332 hasConcept C171250308 @default.
- W3101187332 hasConcept C185592680 @default.
- W3101187332 hasConcept C192562407 @default.
- W3101187332 hasConcept C2776950831 @default.
- W3101187332 hasConcept C2778571141 @default.
- W3101187332 hasConcept C40875361 @default.
- W3101187332 hasConcept C41008148 @default.
- W3101187332 hasConcept C43617362 @default.
- W3101187332 hasConcept C50712719 @default.
- W3101187332 hasConcept C55493867 @default.
- W3101187332 hasConcept C71924100 @default.
- W3101187332 hasConcept C8673954 @default.