FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3101474530> ?p ?o ?g. }

• W3101474530 endingPage "1211" @default.
• W3101474530 startingPage "1211" @default.
• W3101474530 abstract "HomePlant DiseaseVol. 105, No. 4First Report of Leaf Spot Caused by Alternaria alternata on Italian Ryegrass (Lolium multiflorum) in China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Leaf Spot Caused by Alternaria alternata on Italian Ryegrass (Lolium multiflorum) in ChinaX. K. Wei, L. H. Xue, and C. J. LiX. K. WeiState Key Laboratory of Grassland Agro-Ecosystems; Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs; Engineering Research Center of Grassland Industry, Ministry of Education; Gansu Tech Innovation Center of Western China Grassland Industry; Center for Grassland Microbiome; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, ChinaSearch for more papers by this author, L. H. Xue†Corresponding authors: L. H. Xue; E-mail Address: xuelonghai55@126.com and C. J. Li; E-mail Address: chunjie@lzu.edu.cnhttp://orcid.org/0000-0002-1158-2152State Key Laboratory of Grassland Agro-Ecosystems; Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs; Engineering Research Center of Grassland Industry, Ministry of Education; Gansu Tech Innovation Center of Western China Grassland Industry; Center for Grassland Microbiome; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, ChinaSearch for more papers by this author, and C. J. Li†Corresponding authors: L. H. Xue; E-mail Address: xuelonghai55@126.com and C. J. Li; E-mail Address: chunjie@lzu.edu.cnhttp://orcid.org/0000-0002-3287-2140State Key Laboratory of Grassland Agro-Ecosystems; Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs; Engineering Research Center of Grassland Industry, Ministry of Education; Gansu Tech Innovation Center of Western China Grassland Industry; Center for Grassland Microbiome; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, ChinaGrassland Research Center of National Forestry and Grassland Administration, Chinese Academy of Forestry Sciences, Beijing 100091, ChinaSearch for more papers by this author AffiliationsAuthors and Affiliations X. K. Wei1 L. H. Xue1 † C. J. Li1 2 † 1State Key Laboratory of Grassland Agro-Ecosystems; Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs; Engineering Research Center of Grassland Industry, Ministry of Education; Gansu Tech Innovation Center of Western China Grassland Industry; Center for Grassland Microbiome; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China 2Grassland Research Center of National Forestry and Grassland Administration, Chinese Academy of Forestry Sciences, Beijing 100091, China Published Online:17 Feb 2021https://doi.org/10.1094/PDIS-08-20-1737-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleItalian ryegrass (Lolium multiflorum Lam.) is one of the most important forage crops in southwestern China. In 2018, a leaf spot was observed in a field of Italian ryegrass in Mengyang, Sichuan province, China (30.96925°N, 104.10223°E). From January to early March, this leaf spot developed sporadically and appeared as brown to dark brown lesions. In late May, this disease reached a peak with incidence up to 80% and appeared as reddish-brown necrotic spots with a grayish white to brown center. To isolate the pathogen, sections (0.5 × 1 cm) of 30 diseased leaves collected from 10 plants were surface disinfested in 70% ethanol solution for 30 s followed by 5% NaOCl solution for 5 min, rinsed thrice in sterilized distilled water, air dried, plated on potato dextrose agar (PDA), and incubated at 25°C in the dark for 4 days. To obtain pure isolates, the single-spore isolation technique (Cai et al. 2009) was used. The conidial suspensions were diluted to a reasonable concentration, spread onto PDA, and incubated at 25°C in dark for 24 to 48 h, and then single germinated conidia were transferred onto new PDA plates (Cai et al. 2009). Nine pure isolates showing similar morphology were obtained for further study. Colonies on PDA were dark gray in the center surrounded by white to gray, with gossypine mycelia on the upper side, and red to dark red on the reverse side. Conidia were obclavate or pyriform, olivaceous to dark brown, with zero to six transverse septa and zero to four longitudinal septa, 13.2 to 55.0 (27.9) × 6.3 to 12.5 (9.8) µm. Conidiophores were septate, hyaline to olivaceous brown, either branched or unbranched, geniculate at the tip, 2.5 to 5.9 μm wide, and up to 70 μm long. These morphological and cultural characteristics were consistent with the descriptions of Alternaria alternata (Fr.) Keissl. isolated from apple (Elfar et al. 2018). To confirm the pathogenicity on Italian ryegrass, healthy plants (8 weeks old) of cultivar Splendor grown in five pots filled with potting soil were spray inoculated with conidial suspension (1 × 106 conidia/ml). Plants in another five pots were sprayed with sterilized distilled water as controls. All pots were individually covered with transparent polyethylene bags for 5 days to maintain high relative humidity and placed in a greenhouse at 18 to 25°C. At 14 days postinoculation, typical symptoms of brown to dark brown leaf spots developed on the plants inoculated with conidial suspension, whereas no symptoms were observed on the control plants. The pathogenicity tests were carried out three times. The same pathogen was consistently reisolated from inoculated leaves and confirmed by morphological characterization as described above. To further identify this pathogen, isolate HMCH-9 (= CGMCC 3.19924) was selected as a representative for molecular characterization. Following Woudenberg et al. (2015), the internal transcribed spacer regions 1 and 2 and intervening 5.8S rDNA (ITS), glyceraldehyde-3-phosphate dehydrogenase (GPD), translation elongation factor 1-alpha (TEF), RNA polymerase second largest subunit (RPB2), and Alternaria major allergen (Alt) genes were partially amplified and sequenced. Sequences were deposited in GenBank (accession nos. MH567106 for ITS, MH567107 for GPD, MH567109 for TEF, MH567110 for RPB2, and MH567108 for Alt). BLAST analysis of all these five segments showed >99.8% identity with those sequences of ex-type isolate CBS 916.96 of A. alternata (Woudenberg et al. 2015). To our knowledge, this is the first report of A. alternata causing leaf spot on Italian ryegrass in China. The accurate identification of this pathogen would be useful for the prevention and control of leaf spot on Italian ryegrass in the future.The author(s) declare no conflict of interest.References:Cai, L., et al. 2009. Fungal Divers. 39:183. ISI, Google ScholarElfar, K., et al. 2018. Plant Dis. 102:2158. https://doi.org/10.1094/PDIS-02-18-0282-RE Link, ISI, Google ScholarWoudenberg, J. H. C., et al. 2015. Stud. Mycol. 82:1. https://doi.org/10.1016/j.simyco.2015.07.001 Crossref, ISI, Google ScholarThe author(s) declare no conflict of interest.Funding: This research was financially supported by China Agriculture Research System – Forage (CARS-034) and China Agriculture Research System – Green Manure (CARS-022).DetailsFiguresLiterature CitedRelated Vol. 105, No. 4 April 2021SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionLeaf symptom of field-grown ‘Hayward’ kiwifruit plant infected with Actinidia virus (L. Zhao et al.). Photo credit: L. Zhao. Leaf spot of Italian ryegrass naturally infected by Alternaria alternata (X. K. Wei et al.). Photo credit: L. H. Xue. Leaf of Ficus carica displaying symptoms of fig mosaic disease (S. Preising et al.). Photo credit: W. L. da Silva. Metrics Downloaded 703 times Article History Issue Date: 21 Apr 2021Published: 17 Feb 2021First Look: 11 Nov 2020Accepted: 10 Nov 2020 Page: 1211 Information© 2021 The American Phytopathological SocietyFundingChina Agriculture Research System – ForageGrant/Award Number: CARS-034China Agriculture Research System – Green ManureGrant/Award Number: CARS-022Keywordsfungipathogen diversitypathogen detectionThe author(s) declare no conflict of interest.PDF download" @default.
• W3101474530 created "2020-11-23" @default.
• W3101474530 creator A5027937706 @default.
• W3101474530 creator A5059244865 @default.
• W3101474530 creator A5068380836 @default.
• W3101474530 date "2021-04-01" @default.
• W3101474530 modified "2023-09-26" @default.
• W3101474530 title "First Report of Leaf Spot Caused by <i>Alternaria alternata</i> on Italian Ryegrass (<i>Lolium multiflorum</i>) in China" @default.
• W3101474530 cites W1125634552 @default.
• W3101474530 cites W2805509306 @default.
• W3101474530 doi "https://doi.org/10.1094/pdis-08-20-1737-pdn" @default.
• W3101474530 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33174794" @default.
• W3101474530 hasPublicationYear "2021" @default.
• W3101474530 type Work @default.
• W3101474530 sameAs 3101474530 @default.
• W3101474530 citedByCount "3" @default.
• W3101474530 countsByYear W31014745302022 @default.
• W3101474530 countsByYear W31014745302023 @default.
• W3101474530 crossrefType "journal-article" @default.
• W3101474530 hasAuthorship W3101474530A5027937706 @default.
• W3101474530 hasAuthorship W3101474530A5059244865 @default.
• W3101474530 hasAuthorship W3101474530A5068380836 @default.
• W3101474530 hasBestOaLocation W31014745301 @default.
• W3101474530 hasConcept C144027150 @default.
• W3101474530 hasConcept C17744445 @default.
• W3101474530 hasConcept C191935318 @default.
• W3101474530 hasConcept C199539241 @default.
• W3101474530 hasConcept C2779159236 @default.
• W3101474530 hasConcept C2779771029 @default.
• W3101474530 hasConcept C2780034373 @default.
• W3101474530 hasConcept C2780720873 @default.
• W3101474530 hasConcept C59822182 @default.
• W3101474530 hasConcept C6557445 @default.
• W3101474530 hasConcept C86803240 @default.
• W3101474530 hasConceptScore W3101474530C144027150 @default.
• W3101474530 hasConceptScore W3101474530C17744445 @default.
• W3101474530 hasConceptScore W3101474530C191935318 @default.
• W3101474530 hasConceptScore W3101474530C199539241 @default.
• W3101474530 hasConceptScore W3101474530C2779159236 @default.
• W3101474530 hasConceptScore W3101474530C2779771029 @default.
• W3101474530 hasConceptScore W3101474530C2780034373 @default.
• W3101474530 hasConceptScore W3101474530C2780720873 @default.
• W3101474530 hasConceptScore W3101474530C59822182 @default.
• W3101474530 hasConceptScore W3101474530C6557445 @default.
• W3101474530 hasConceptScore W3101474530C86803240 @default.
• W3101474530 hasIssue "4" @default.
• W3101474530 hasLocation W31014745301 @default.
• W3101474530 hasLocation W31014745302 @default.
• W3101474530 hasOpenAccess W3101474530 @default.
• W3101474530 hasPrimaryLocation W31014745301 @default.
• W3101474530 hasRelatedWork W1578443608 @default.
• W3101474530 hasRelatedWork W1753019555 @default.
• W3101474530 hasRelatedWork W2016315421 @default.
• W3101474530 hasRelatedWork W2298424742 @default.
• W3101474530 hasRelatedWork W2466125300 @default.
• W3101474530 hasRelatedWork W2811373640 @default.
• W3101474530 hasRelatedWork W2957108685 @default.
• W3101474530 hasRelatedWork W3012002703 @default.
• W3101474530 hasRelatedWork W3027791036 @default.
• W3101474530 hasRelatedWork W1586083311 @default.
• W3101474530 hasVolume "105" @default.
• W3101474530 isParatext "false" @default.
• W3101474530 isRetracted "false" @default.
• W3101474530 magId "3101474530" @default.
• W3101474530 workType "article" @default.