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- W3101857 abstract "This chapter discusses the effect of Con A on the cation affinity of the Na + –K + pump in lymphocytes. The chapter describes a study that was carried out to find out whether the mitogen-induced increase of Na + and decrease of K + concentrations are responsible for the enhanced K + uptake and Na + efflux in lymphocytes. It was conceivable that a decreased Na + concentration in the culture medium may prevent the mitogen-induced increase in cellular Na + . For this study, RPMI medium was used in which Na + was replaced by Hepes/Tris pH 7.4. The chapter also illustrates the intracellular Na + and K + concentration of mouse thymocytes incubated in media of different Na + concentrations. In comparison to the experiments carried out in high Na + media, cellular K + levels were 30% lower after one hour of incubation in 5 mM Na + media. The intracellular Na + concentrations increased in media with 120 mM Na + within the first 180 minutes and decreased in low Na + media. The cytocrit, as an indicator of cell volume, decreased in the 5 mM Na + buffer by about 12%. Con A had no additional effect on cell volume in any medium." @default.
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- W3101857 date "1979-01-01" @default.
- W3101857 modified "2023-09-27" @default.
- W3101857 title "EFFECT OF CON-A ON THE CATION AFFINITY OF THE Na+- K+ PUMP IN LYMPHOCYTES" @default.
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- W3101857 doi "https://doi.org/10.1016/b978-0-12-569650-0.50014-0" @default.
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