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- W3102042143 abstract "A set of four luminescent EuIII complexes bearing an extended aryl-alkynylpyridine chromophore has been studied, showing very different pH-dependent behaviour in their absorption and emission spectral response. For two complexes with pKa values of 6.45 and 6.20 in protein-containing solution, the emission lifetime increases very significantly following protonation. By varying the gate time during signal acquisition, the ‘switch-on’ intensity ratio could be optimised, and enhancement factors of between 250 to 1330 were measured between pH 8 and 4. The best-behaved probe showed no significant emission dependence on the concentration of endogenous cations, reductants, and serum albumin. It was examined in live-cell imaging studies to monitor time-dependent lysosomal acidification, for which the increase in observed image brightness due to acidification was a factor of 50 in NIH-3T3 cells." @default.
- W3102042143 created "2020-11-23" @default.
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- W3102042143 date "2020-12-15" @default.
- W3102042143 modified "2023-10-11" @default.
- W3102042143 title "Synthesis and Evaluation of Europium Complexes that Switch on Luminescence in Lysosomes of Living Cells" @default.
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- W3102042143 doi "https://doi.org/10.1002/chem.202003992" @default.
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