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- W3102082362 abstract "Light-inducible dimerization protein modules enable precise temporal and spatial control of biological processes in non-invasive fashion. Among them, Magnets are small modules engineered from the Neurospora crassa photoreceptor Vivid by orthogonalizing the homodimerization interface into complementary heterodimers. Both Magnets components, which are well-tolerated as protein fusion partners, are photoreceptors requiring simultaneous photoactivation to interact, enabling high spatiotemporal confinement of dimerization with a single excitation wavelength. However, Magnets require concatemerization for efficient responses and cell preincubation at 28°C to be functional. Here we overcome these limitations by engineering an optimized Magnets pair requiring neither concatemerization nor low temperature preincubation. We validated these ‘enhanced’ Magnets (eMags) by using them to rapidly and reversibly recruit proteins to subcellular organelles, to induce organelle contacts, and to reconstitute OSBP-VAP ER-Golgi tethering implicated in phosphatidylinositol-4-phosphate transport and metabolism. eMags represent a very effective tool to optogenetically manipulate physiological processes over whole cells or in small subcellular volumes." @default.
- W3102082362 created "2020-11-23" @default.
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- W3102082362 date "2020-11-11" @default.
- W3102082362 modified "2023-10-17" @default.
- W3102082362 title "Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells" @default.
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- W3102082362 doi "https://doi.org/10.7554/elife.63230" @default.
- W3102082362 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7735757" @default.
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