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- W3102104046 abstract "Objective To investigate the genes involved in Wolbachia -induced cytoplasmic incompatibility among three natural populations of Culex pipiens pallens in eastern China, so as to provide insights into the development of preventive and control measures for mosquito-borne diseases based on Wolbachia . Methods The cytoplasmic incompatibility was tested among three natural populations of C. pipiens pallens collected from Nanjing and Wuxi of Jiangsu Province and Tangkou of Shandong Province using reciprocal crosses. Wolbachi a infection was detected in C. pipiens pallens using a PCR assay, and the expression of Wolbachia wsp and WD0513 genes was quantified using a fluorescent quantitative real-time PCR (qPCR) assay. Results Bidirectional compatibility was found between the natural populations of C. pipiens pallens collected from Nanjing and Wuxi of Jiangsu Province ( t = 0.57 and 0.15, both P values > 0.05), while bidirectional incompatibility was seen between the natural populations of C. pipiens pallens collected from Tangkou of Shandong Province and Wuxi of Jiangsu Province ( t = 63.81 and 43.51, both P values 0.05). In addition, there was no significant difference in the WD0513 gene expression between the natural populations of C. pipiens pallens collected from Tangkou of Shandong Province and Nanjing of Jiangsu Province ( q = 8.42, P 0.05). Conclusion Different Wolbachia numbers are detected in natural populations of C. pipiens pallens collected from Nanjing and Wuxi of Jiangsu Province and Tangkou of Shandong Province, and WD0513 gene may be involved in the Wolbachia -induced cytoplasmic incompatibility among three natural populations of C. pipiens pallens . ［摘要］ 目的 探讨我国东部地区3个地理种群淡色库蚊由沃尔巴克氏体导致细胞质不亲和的相关基因, 为制定基于 沃尔巴克氏体的蚊媒病防制措施提供参考。 方法 采用正反交实验确定江苏南京、江苏无锡和山东唐口3个淡色库蚊 自然种群间细胞质不亲和, 应用PCR技术检测淡色库蚊沃尔巴克氏体感染情况, 采用荧光定量PCR法检测沃尔巴克氏 体 wsp 基因和沃尔巴克氏体基因组中Octomom增殖区域 WD0513 基因表达水平。 结果 江苏南京与无锡淡色库蚊自然 种群双向亲和 ( t = 0.57、0.15, P 均> 0.05), 山东唐口和江苏无锡淡色库蚊自然种群双向不亲和 ( t = 63.81、43.51, P 均 0.05) 。山东唐口与江苏南京、山东唐口与江苏无锡淡色库蚊自然种群 WD0513 基因表 达水平间差异均有统计学意义 ( q = 8.42、7.84, P 均 0.05) 。 结论 江苏南京、江苏无锡和山东唐口3个地理种群淡色库蚊所感染沃 尔巴克氏体数量不同, 淡色库蚊自然种群感染沃尔巴克氏体导致的细胞质不亲和可能与 WD0513 基因表达量相关。" @default.
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- W3102104046 date "2020-10-16" @default.
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- W3102104046 title "[Genes associated with Wolbachia-induced cytoplasmic incompatibility in natural populations of Culex pipiens pallen s: a preliminary study]" @default.
- W3102104046 doi "https://doi.org/10.16250/j.32.1374.2019197" @default.
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