Matches in SemOpenAlex for { <https://semopenalex.org/work/W3102163077> ?p ?o ?g. }
- W3102163077 abstract "Abstract Dendritic cells (DCs) play a pivotal role to amplify antigen-specific immune responses. Antigens that sensitize T cells via antigen-presentation by DCs could enhance the capacity of host immunity to fight infections. In this study, we tested the immunogenic profiles of chicken DCs towards Glyceraldehyde-3-phosphate dehydrogenase from Eimeria acervulina (EaGAPDH). Immunoblot analysis showed that recombinant EaGAPDH (rEaGAPDH) protein was successfully recognized by rat sera generated against rEaGAPDH. Interaction and internalisation of rEaGAPDH by chicken splenic-derived DCs (chSPDCs) was confirmed by immunofluorescence analysis. Flow cytometry revealed that chSPDCs upregulated MHCII, CD1.1, CD11c, CD80, and CD86 cell-surface markers. Moreover, mRNA expressions of DC maturation biomarkers (CCL5, CCR7, and CD83) and TLR signalling genes (TLR15 and MyD88) were also upregulated whereas those of Wnt signalling were non-significant compared to negative controls. rEaGAPDH treatment induced IL-12 and IFN-γ secretion in chSPDCs but had no effect on IL-10 and TGF-β. Likewise, DC-T cell co-culture promoted IFN-γ secretion and the level of IL-4 was unaffected. Proliferation of T cells and their differentiation into CD3 + /CD4 + T cells were triggered in chSPDCs-T cells co-culture system. Taken together, rEaGAPDH could promote Th1 polarization by activating both host DCs and T cells and sheds new light on the role of this important molecule which might contribute to the development of new DCs-based immunotherapeutic strategies against coccidiosis." @default.
- W3102163077 created "2020-11-23" @default.
- W3102163077 creator A5015847699 @default.
- W3102163077 creator A5019358491 @default.
- W3102163077 creator A5028551178 @default.
- W3102163077 creator A5031351655 @default.
- W3102163077 creator A5033133176 @default.
- W3102163077 creator A5065786027 @default.
- W3102163077 creator A5066281981 @default.
- W3102163077 creator A5068261466 @default.
- W3102163077 creator A5073190623 @default.
- W3102163077 creator A5079824739 @default.
- W3102163077 date "2020-11-17" @default.
- W3102163077 modified "2023-09-26" @default.
- W3102163077 title "Glyceraldehyde-3-phosphate dehydrogenase from Eimeria acervulina modulates the functions of chicken dendritic cells to boost Th1 type immune response and stimulates autologous CD4+ T cells differentiation in-vitro" @default.
- W3102163077 cites W104146476 @default.
- W3102163077 cites W1548322053 @default.
- W3102163077 cites W1661544713 @default.
- W3102163077 cites W1666558076 @default.
- W3102163077 cites W1982293312 @default.
- W3102163077 cites W1982637996 @default.
- W3102163077 cites W1985497488 @default.
- W3102163077 cites W1987263704 @default.
- W3102163077 cites W1993142414 @default.
- W3102163077 cites W2002761249 @default.
- W3102163077 cites W2004805241 @default.
- W3102163077 cites W2006419043 @default.
- W3102163077 cites W2014956761 @default.
- W3102163077 cites W2022179150 @default.
- W3102163077 cites W2026024028 @default.
- W3102163077 cites W2035484798 @default.
- W3102163077 cites W2041489554 @default.
- W3102163077 cites W2049165816 @default.
- W3102163077 cites W2063882056 @default.
- W3102163077 cites W2066458665 @default.
- W3102163077 cites W2074183668 @default.
- W3102163077 cites W2074391032 @default.
- W3102163077 cites W2077883112 @default.
- W3102163077 cites W2082073529 @default.
- W3102163077 cites W2089664032 @default.
- W3102163077 cites W2103644250 @default.
- W3102163077 cites W2107277218 @default.
- W3102163077 cites W2116403793 @default.
- W3102163077 cites W2117929276 @default.
- W3102163077 cites W2120787532 @default.
- W3102163077 cites W2130832456 @default.
- W3102163077 cites W2155697606 @default.
- W3102163077 cites W2159153132 @default.
- W3102163077 cites W2163269741 @default.
- W3102163077 cites W2302910861 @default.
- W3102163077 cites W2343080363 @default.
- W3102163077 cites W2517664818 @default.
- W3102163077 cites W2604210932 @default.
- W3102163077 cites W2738511953 @default.
- W3102163077 cites W2801774047 @default.
- W3102163077 cites W2901012444 @default.
- W3102163077 cites W2921693673 @default.
- W3102163077 cites W2990139186 @default.
- W3102163077 cites W3010805801 @default.
- W3102163077 cites W3010983188 @default.
- W3102163077 cites W4249383371 @default.
- W3102163077 doi "https://doi.org/10.1186/s13567-020-00864-z" @default.
- W3102163077 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7672913" @default.
- W3102163077 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33203464" @default.
- W3102163077 hasPublicationYear "2020" @default.
- W3102163077 type Work @default.
- W3102163077 sameAs 3102163077 @default.
- W3102163077 citedByCount "3" @default.
- W3102163077 countsByYear W31021630772022 @default.
- W3102163077 crossrefType "journal-article" @default.
- W3102163077 hasAuthorship W3102163077A5015847699 @default.
- W3102163077 hasAuthorship W3102163077A5019358491 @default.
- W3102163077 hasAuthorship W3102163077A5028551178 @default.
- W3102163077 hasAuthorship W3102163077A5031351655 @default.
- W3102163077 hasAuthorship W3102163077A5033133176 @default.
- W3102163077 hasAuthorship W3102163077A5065786027 @default.
- W3102163077 hasAuthorship W3102163077A5066281981 @default.
- W3102163077 hasAuthorship W3102163077A5068261466 @default.
- W3102163077 hasAuthorship W3102163077A5073190623 @default.
- W3102163077 hasAuthorship W3102163077A5079824739 @default.
- W3102163077 hasBestOaLocation W31021630771 @default.
- W3102163077 hasConcept C104317684 @default.
- W3102163077 hasConcept C116484652 @default.
- W3102163077 hasConcept C127716648 @default.
- W3102163077 hasConcept C147483822 @default.
- W3102163077 hasConcept C153911025 @default.
- W3102163077 hasConcept C154317977 @default.
- W3102163077 hasConcept C202751555 @default.
- W3102163077 hasConcept C203014093 @default.
- W3102163077 hasConcept C2776090121 @default.
- W3102163077 hasConcept C2778170410 @default.
- W3102163077 hasConcept C2778297628 @default.
- W3102163077 hasConcept C2779919695 @default.
- W3102163077 hasConcept C2781101188 @default.
- W3102163077 hasConcept C3753494 @default.
- W3102163077 hasConcept C39347974 @default.
- W3102163077 hasConcept C55493867 @default.
- W3102163077 hasConcept C67662055 @default.
- W3102163077 hasConcept C79484868 @default.
- W3102163077 hasConcept C83464605 @default.