FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3102293742> ?p ?o ?g. }

• W3102293742 abstract "Abstract This study sets out to establish the suitability of saliva-based whole-genome sequencing (WGS) through a comparison against blood-based WGS. To fully appraise the observed differences, we developed a novel technique of pseudo-replicates. We also investigated the potential of characterising individual salivary microbiomes from non-human DNA fragments found in saliva. We observed the majority of discordant genotype calls between blood and saliva calls fell into known regions of the human genome that are typically sequenced with low confidence; and could be identified by quality control measures. Pseudo-replication demonstrated that the levels of discordance between blood- and saliva-derived WGS data were entirely similar to what one would expect between technical replicates if an individual’s blood or saliva had been sequenced twice. Finally, we successfully sequenced salivary microbiomes in parallel to human genomes as demonstrated by a comparison against the Human Microbiome Project. Author Summary DNA is usually collected from blood for the analysis of human genomes. In France, a new and very large genetic dataset will be created where selected participants will be sent saliva-collection kits in the post as this data collection method presents numerous logistical benefits. It has been previously shown that good quality genetic data can be created from saliva, though existing studies have often not considered the latest technologies or have only analysed a very small number of individuals. In this study, we have analysed genetic data derived from saliva for 39 individuals to give a firm conclusion that the proposed genome sequencing approach of the new French dataset will be capable of provided high quality data by making a comparison to pre-existing genetic data derived from blood for these 39 individuals. In order to do so, we developed a novel method (presented here) to establish the similarity between two sets of genetic data for the same individual that are generated from separate DNA samples. Finally, we have also demonstrated an added bonus of colleting saliva samples: that it is possible to gather both human genetic data and potentially interesting salivary microbiome data at the same time by separating and analysing in parallel human and non-human DNA fragments." @default.
• W3102293742 created "2020-11-23" @default.
• W3102293742 creator A5008671699 @default.
• W3102293742 creator A5011031207 @default.
• W3102293742 creator A5011453874 @default.
• W3102293742 creator A5015449055 @default.
• W3102293742 creator A5024251772 @default.
• W3102293742 creator A5027469318 @default.
• W3102293742 creator A5028873076 @default.
• W3102293742 creator A5037363695 @default.
• W3102293742 creator A5049107218 @default.
• W3102293742 creator A5058691508 @default.
• W3102293742 creator A5065730986 @default.
• W3102293742 creator A5075838907 @default.
• W3102293742 creator A5078726565 @default.
• W3102293742 date "2020-11-16" @default.
• W3102293742 modified "2023-10-06" @default.
• W3102293742 title "Evaluation of saliva as a source of accurate whole-genome and microbiome sequencing data" @default.
• W3102293742 cites W1964079064 @default.
• W3102293742 cites W1987869189 @default.
• W3102293742 cites W1988431250 @default.
• W3102293742 cites W2011099053 @default.
• W3102293742 cites W2034285706 @default.
• W3102293742 cites W2056279562 @default.
• W3102293742 cites W2078323312 @default.
• W3102293742 cites W2079979147 @default.
• W3102293742 cites W2082704080 @default.
• W3102293742 cites W2084336300 @default.
• W3102293742 cites W2103441770 @default.
• W3102293742 cites W2116779663 @default.
• W3102293742 cites W2120902911 @default.
• W3102293742 cites W2122767606 @default.
• W3102293742 cites W2123263006 @default.
• W3102293742 cites W2128769815 @default.
• W3102293742 cites W2141682018 @default.
• W3102293742 cites W2142858065 @default.
• W3102293742 cites W2147733682 @default.
• W3102293742 cites W2148412596 @default.
• W3102293742 cites W2153137490 @default.
• W3102293742 cites W2153712024 @default.
• W3102293742 cites W2160511389 @default.
• W3102293742 cites W2160724951 @default.
• W3102293742 cites W2167964622 @default.
• W3102293742 cites W2168133698 @default.
• W3102293742 cites W2190569576 @default.
• W3102293742 cites W2326641739 @default.
• W3102293742 cites W2334884587 @default.
• W3102293742 cites W2467325308 @default.
• W3102293742 cites W2510766756 @default.
• W3102293742 cites W2511515754 @default.
• W3102293742 cites W2528374605 @default.
• W3102293742 cites W2528619219 @default.
• W3102293742 cites W2560652310 @default.
• W3102293742 cites W2769542288 @default.
• W3102293742 cites W2779975541 @default.
• W3102293742 cites W2784047162 @default.
• W3102293742 cites W2789516263 @default.
• W3102293742 cites W2792381148 @default.
• W3102293742 cites W2910199421 @default.
• W3102293742 cites W2914547442 @default.
• W3102293742 cites W2924660869 @default.
• W3102293742 cites W2929354011 @default.
• W3102293742 cites W2951529834 @default.
• W3102293742 cites W2951576702 @default.
• W3102293742 cites W2952191974 @default.
• W3102293742 cites W2952870794 @default.
• W3102293742 cites W2961404751 @default.
• W3102293742 cites W2972731224 @default.
• W3102293742 cites W2990618091 @default.
• W3102293742 cites W3007907037 @default.
• W3102293742 cites W3024875793 @default.
• W3102293742 doi "https://doi.org/10.1101/2020.11.16.384438" @default.
• W3102293742 hasPublicationYear "2020" @default.
• W3102293742 type Work @default.
• W3102293742 sameAs 3102293742 @default.
• W3102293742 citedByCount "0" @default.
• W3102293742 crossrefType "posted-content" @default.
• W3102293742 hasAuthorship W3102293742A5008671699 @default.
• W3102293742 hasAuthorship W3102293742A5011031207 @default.
• W3102293742 hasAuthorship W3102293742A5011453874 @default.
• W3102293742 hasAuthorship W3102293742A5015449055 @default.
• W3102293742 hasAuthorship W3102293742A5024251772 @default.
• W3102293742 hasAuthorship W3102293742A5027469318 @default.
• W3102293742 hasAuthorship W3102293742A5028873076 @default.
• W3102293742 hasAuthorship W3102293742A5037363695 @default.
• W3102293742 hasAuthorship W3102293742A5049107218 @default.
• W3102293742 hasAuthorship W3102293742A5058691508 @default.
• W3102293742 hasAuthorship W3102293742A5065730986 @default.
• W3102293742 hasAuthorship W3102293742A5075838907 @default.
• W3102293742 hasAuthorship W3102293742A5078726565 @default.
• W3102293742 hasBestOaLocation W31022937421 @default.
• W3102293742 hasConcept C104317684 @default.
• W3102293742 hasConcept C141231307 @default.
• W3102293742 hasConcept C143121216 @default.
• W3102293742 hasConcept C197077220 @default.
• W3102293742 hasConcept C24432333 @default.
• W3102293742 hasConcept C504460877 @default.
• W3102293742 hasConcept C51679486 @default.
• W3102293742 hasConcept C54355233 @default.
• W3102293742 hasConcept C552990157 @default.

• next