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• W3102374795 endingPage "8572" @default.
• W3102374795 startingPage "8572" @default.
• W3102374795 abstract "Purinergic receptors of the P2 subclass are commonly found in human and rodent macrophages where they can be activated by adenosine 5′-triphosphate (ATP) or uridine 5′-triphosphate (UTP) to mediate Ca2+ mobilization, resulting in downstream signalling to promote inflammation and pain. However, little is understood regarding these receptors in canine macrophages. To establish a macrophage model of canine P2 receptor signalling, the expression of these receptors in the DH82 canine macrophage cell line was determined by reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemistry. P2 receptor function in DH82 cells was pharmacologically characterised using nucleotide-induced measurements of Fura-2 AM-bound intracellular Ca2+. RT-PCR revealed predominant expression of P2X4 receptors, while immunocytochemistry confirmed predominant expression of P2Y2 receptors, with low levels of P2X4 receptor expression. ATP and UTP induced robust Ca2+ responses in the absence or presence of extracellular Ca2+. ATP-induced responses were only partially inhibited by the P2X4 receptor antagonists, 2′,3′-O-(2,4,6-trinitrophenyl)-ATP (TNP-ATP), paroxetine and 5-BDBD, but were strongly potentiated by ivermectin. UTP-induced responses were near completely inhibited by the P2Y2 receptor antagonists, suramin and AR-C118925. P2Y2 receptor-mediated Ca2+ mobilization was inhibited by U-73122 and 2-aminoethoxydiphenyl borate (2-APB), indicating P2Y2 receptor coupling to the phospholipase C and inositol triphosphate signal transduction pathway. Together this data demonstrates, for the first time, the expression of functional P2 receptors in DH82 canine macrophage cells and identifies a potential cell model for studying macrophage-mediated purinergic signalling in inflammation and pain in dogs." @default.
• W3102374795 created "2020-11-23" @default.
• W3102374795 creator A5041363133 @default.
• W3102374795 creator A5052350687 @default.
• W3102374795 creator A5054821864 @default.
• W3102374795 creator A5063117675 @default.
• W3102374795 date "2020-11-13" @default.
• W3102374795 modified "2023-10-06" @default.
• W3102374795 title "P2Y2 and P2X4 Receptors Mediate Ca2+ Mobilization in DH82 Canine Macrophage Cells" @default.
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• W3102374795 doi "https://doi.org/10.3390/ijms21228572" @default.
• W3102374795 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7696671" @default.
• W3102374795 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33202978" @default.

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