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• W3102481877 abstract "Advances in DNA sequencing and informatics have revolutionised biology over the past four decades, but technological limitations have left many applications unexplored 1,2 . Recently, portable, real-time, nanopore sequencing (RTnS) has become available. This offers opportunities to rapidly collect and analyse genomic data anywhere 3–5 . However, the generation of datasets from large, complex genomes has been constrained to laboratories 6,7 . The portability and long DNA sequences of RTnS offer great potential for field-based species identification, but the feasibility and accuracy of these technologies for this purpose have not been assessed. Here, we show that a field-based RTnS analysis of closely-related plant species ( Arabidopsis spp .) 8 has many advantages over laboratory-based high-throughput sequencing (HTS) methods for species level identification-by-sequencing and de novo phylogenomics. Samples were collected and sequenced in a single day by RTnS using a portable, “al fresco” laboratory. Our analyses demonstrate that correctly identifying unknown reads from matches to a reference database with RTnS reads enables rapid and confident species identification. Individually annotated RTnS reads can be used to infer the evolutionary relationships of A. thaliana . Furthermore, hybrid genome assembly with RTnS and HTS reads substantially improved upon a genome assembled from HTS reads alone. Field-based RTnS makes real-time, rapid specimen identification and genome wide analyses possible. These technological advances are set to revolutionise research in the biological sciences 9 and have broad implications for conservation, taxonomy, border agencies and citizen science." @default.
• W3102481877 created "2020-11-23" @default.
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• W3102481877 date "2017-02-10" @default.
• W3102481877 modified "2023-09-26" @default.
• W3102481877 title "Field-based species identification in eukaryotes using real-time nanopore sequencing" @default.
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• W3102481877 doi "https://doi.org/10.1101/107656" @default.
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