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- W310333015 abstract "Solid-phase radioimmunoassay (SPRIA) techniques have been valuable for quantifying antibody to bacteria (I) and viruses (2,3). In the indirect solid-phase radioimmunoassay technique, a purified antigen is usually attached directly to the solid phase. The attached antigen is then exposed to the human test serum; and after thorough washing, antibodies combined with the antigen are detected by means of radioisotopically labeled antibodies specific for human IgM or IgG. Application of these techniques for determining the presence of human antibody to Yez exquisite sensitivityon the order of 0.5 pg of antibody per ml (I); and the ability to measure both IgM and IgG responses. The soluble protein fraction 1A of Y. pestis (4), recommended for serodiagnosis of human plague infection (5), constitutes a highly purified specific antigen; but it is only available in limited quantities from noncommercial sources. The most sensitive serologic test now used for diagnosis of human plague, the passive hemagglutination (PHA) test (5)," @default.
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- W310333015 date "1980-01-01" @default.
- W310333015 modified "2023-09-23" @default.
- W310333015 title "The use of solid-phase radioimmunoassay techniques for serodiagnosis of human plague infection." @default.
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