Matches in SemOpenAlex for { <https://semopenalex.org/work/W3103399553> ?p ?o ?g. }
- W3103399553 endingPage "417" @default.
- W3103399553 startingPage "417" @default.
- W3103399553 abstract "Our recent study detected the expression of a tissue renin–angiotensin system (tRAS) in human intervertebral discs (IVDs). The present study sought to investigate the impact of the angiotensin II receptor type 1 (AGTR1) antagonist losartan on human nucleus pulposus (NP) cell inflammation and degeneration induced by tumor necrosis factor-α (TNF-α). Human NP cells (4 donors; Pfirrmann grade 2–3; 30–37-years–old; male) were isolated and expanded. TNF-α (10 ng/mL) was used to induce inflammation and degeneration. We examined the impact of losartan supplementation and measured gene expression of tRAS, anabolic, catabolic, and inflammatory markers in NP cells after 24 and 72 h of exposure. T0070907, a PPAR gamma antagonist, was applied to examine the regulatory pathway of losartan. Losartan (1 mM) significantly impaired the TNF-α-induced increase of pro-inflammatory (nitric oxide and TNF-α), catabolic (matrix metalloproteinases), and tRAS (AGTR1a and angiotensin-converting enzyme) markers. Further, losartan maintained the NP cell phenotype by upregulating aggrecan and downregulating collagen type I expression. In summary, losartan showed anti-inflammatory, anti-catabolic, and positive phenotype-modulating effects on human NP cells. These results indicate that tRAS signaling plays an important role in IVD degeneration, and tRAS modulation with losartan could represent a novel therapeutic approach." @default.
- W3103399553 created "2020-11-23" @default.
- W3103399553 creator A5001667773 @default.
- W3103399553 creator A5010012576 @default.
- W3103399553 creator A5016932546 @default.
- W3103399553 creator A5036526404 @default.
- W3103399553 creator A5039482894 @default.
- W3103399553 creator A5040342612 @default.
- W3103399553 creator A5044949872 @default.
- W3103399553 creator A5053261522 @default.
- W3103399553 creator A5054453691 @default.
- W3103399553 creator A5064347608 @default.
- W3103399553 creator A5081236962 @default.
- W3103399553 creator A5082211352 @default.
- W3103399553 creator A5085056832 @default.
- W3103399553 date "2021-01-04" @default.
- W3103399553 modified "2023-09-27" @default.
- W3103399553 title "Angiotensin II Type 1 Receptor Antagonist Losartan Inhibits TNF-α-Induced Inflammation and Degeneration Processes in Human Nucleus Pulposus Cells" @default.
- W3103399553 cites W1511547559 @default.
- W3103399553 cites W1843451178 @default.
- W3103399553 cites W1965455057 @default.
- W3103399553 cites W1992549364 @default.
- W3103399553 cites W1996109225 @default.
- W3103399553 cites W2000563697 @default.
- W3103399553 cites W2001084782 @default.
- W3103399553 cites W2003083924 @default.
- W3103399553 cites W2004591427 @default.
- W3103399553 cites W2005485949 @default.
- W3103399553 cites W2006244753 @default.
- W3103399553 cites W2009826185 @default.
- W3103399553 cites W2013571586 @default.
- W3103399553 cites W2023313940 @default.
- W3103399553 cites W2028439883 @default.
- W3103399553 cites W2039177386 @default.
- W3103399553 cites W2047256051 @default.
- W3103399553 cites W2047847740 @default.
- W3103399553 cites W2048748448 @default.
- W3103399553 cites W2049635464 @default.
- W3103399553 cites W2050062150 @default.
- W3103399553 cites W2052265065 @default.
- W3103399553 cites W2055889289 @default.
- W3103399553 cites W2056561920 @default.
- W3103399553 cites W2060256297 @default.
- W3103399553 cites W2063597031 @default.
- W3103399553 cites W2072052992 @default.
- W3103399553 cites W2072686110 @default.
- W3103399553 cites W2075604884 @default.
- W3103399553 cites W2081398865 @default.
- W3103399553 cites W2085673586 @default.
- W3103399553 cites W2087770419 @default.
- W3103399553 cites W2089118929 @default.
- W3103399553 cites W2090150130 @default.
- W3103399553 cites W2092773978 @default.
- W3103399553 cites W2092798278 @default.
- W3103399553 cites W2096491169 @default.
- W3103399553 cites W2098054841 @default.
- W3103399553 cites W2101726533 @default.
- W3103399553 cites W2101786682 @default.
- W3103399553 cites W2101924426 @default.
- W3103399553 cites W21105706 @default.
- W3103399553 cites W2115096980 @default.
- W3103399553 cites W2117853190 @default.
- W3103399553 cites W2118110481 @default.
- W3103399553 cites W2124579702 @default.
- W3103399553 cites W2126340219 @default.
- W3103399553 cites W2129492896 @default.
- W3103399553 cites W2133694559 @default.
- W3103399553 cites W2138101248 @default.
- W3103399553 cites W2152983876 @default.
- W3103399553 cites W2153785959 @default.
- W3103399553 cites W2161310292 @default.
- W3103399553 cites W2168830792 @default.
- W3103399553 cites W2169958375 @default.
- W3103399553 cites W2171916504 @default.
- W3103399553 cites W2277966459 @default.
- W3103399553 cites W2296254759 @default.
- W3103399553 cites W2325408458 @default.
- W3103399553 cites W2541750414 @default.
- W3103399553 cites W2612188380 @default.
- W3103399553 cites W2751884637 @default.
- W3103399553 cites W2752637575 @default.
- W3103399553 cites W2792601250 @default.
- W3103399553 cites W2881853507 @default.
- W3103399553 cites W2886312852 @default.
- W3103399553 cites W2890763118 @default.
- W3103399553 cites W2890863884 @default.
- W3103399553 cites W2945373073 @default.
- W3103399553 cites W2966026491 @default.
- W3103399553 cites W2980923221 @default.
- W3103399553 cites W3000441976 @default.
- W3103399553 cites W3034573444 @default.
- W3103399553 cites W3036231571 @default.
- W3103399553 cites W3091711968 @default.
- W3103399553 cites W3101556161 @default.
- W3103399553 doi "https://doi.org/10.3390/app11010417" @default.
- W3103399553 hasPublicationYear "2021" @default.
- W3103399553 type Work @default.
- W3103399553 sameAs 3103399553 @default.