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- W3103441428 abstract "CRISPR-Cas9 gene editing has made it possible to specifically edit genes in a myriad of target cells. Here, a method for isoform-specific editing and clonal selection in Madin-Darby canine kidney (MDCK) epithelial cells is described in detail. This approach was used to address a long-standing question in virology of how adenovirus enters polarized epithelia from the apical surface. Our method relies on selecting two sgRNA sequences, cloning them into a suitable fluorescently labeled Cas9 vector system, and subsequently transfecting our MDCK epithelium and selecting isoform-specific Coxsackievirus and adenovirus receptor knockout clones. Utilization of this method is readily applicable to many other genetic targets in epithelial cells. Simultaneous utilization of an sgRNA upstream and an sgRNA downstream of a target sequence allows for deletion of the intervening sequence, including whole exons. Sorting of cells positive for fluorescent marker gene expression enhances the identification of partial and biallelic gene knockout. PCR screening allows relatively fast and efficient determination of isoform-specific deletion." @default.
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- W3103441428 date "2020-01-01" @default.
- W3103441428 modified "2023-09-23" @default.
- W3103441428 title "Production of isoform-specific knockdown/knockout Madin–Darby canine kidney epithelial cells using CRISPR/Cas9" @default.
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- W3103441428 doi "https://doi.org/10.1016/j.mex.2020.101149" @default.
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