FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3104023786> ?p ?o ?g. }

• W3104023786 abstract "Flow cytometry is one of the most sophisticated technologies for the study of cellular biology, with the unique ability to analyze cell populations numbering in the millions. In addition to their great speed, flow cytometers can be configured with 10 or more detectors, so each cell can be characterized by multiple parameters corresponding to light scattered or emitted as they pass through the system. These capabilities have led to the development of a wide variety of applications for flow cytometry in cell biology, hematology, immunology, oncology, cell culture, and other fields. Nevertheless, a wide range of applications are not suited to flow cytometric analysis because the resulting data fail to capture cell morphology or the spatial distribution of signals within a cell. In contrast to conventional flow cytometry, microscopy can elucidate cell morphology by a number of means, including absorbed light imaging (brightfield), scattered light imaging (darkfield), and fluorescence imaging. An image of a typical mammalian cell measuring approximately 10 mm in diameter will cover over 300 pixels, assuming a detector resolution of 0.5mm per pixel. Hence, even a single cell image represents orders of magnitude more data than is acquired by a flow cytometric analysis of that same cell and therefore may represent far more information about that cell. In theory, a set of brightfield, darkfield, and fluorescence images of each cell would provide all the information of flow cytometry plus morphological features, such as the nuclear to cytoplasmic ratio, membrane texture, the distribution of a fluorescent probe, and so forth. However, as a practical matter, it is difficult to acquire and compare more than a few images of a cell because of the need to change fluorescence filter combinations, reconfigure the microscope for brightfield and darkfield imaging, and register the various images to compensate for shifts and distortion from the different optical arrangements. As a result, flow cytometry and microscopy have remained complementary techniques. The goal of imaging cells in flow has been elusive, despite several approaches that have achieved various levels of success over the last several decades." @default.
• W3104023786 created "2020-11-23" @default.
• W3104023786 creator A5034630760 @default.
• W3104023786 date "2005-09-22" @default.
• W3104023786 modified "2023-09-27" @default.
• W3104023786 title "Multispectral Imaging in Flow: A Technique for Advanced Cellular Studies" @default.
• W3104023786 doi "https://doi.org/10.1093/oso/9780195183146.003.0008" @default.
• W3104023786 hasPublicationYear "2005" @default.
• W3104023786 type Work @default.
• W3104023786 sameAs 3104023786 @default.
• W3104023786 citedByCount "0" @default.
• W3104023786 crossrefType "book-chapter" @default.
• W3104023786 hasAuthorship W3104023786A5034630760 @default.
• W3104023786 hasConcept C120665830 @default.
• W3104023786 hasConcept C121332964 @default.
• W3104023786 hasConcept C147080431 @default.
• W3104023786 hasConcept C1491633281 @default.
• W3104023786 hasConcept C153911025 @default.
• W3104023786 hasConcept C154945302 @default.
• W3104023786 hasConcept C160633673 @default.
• W3104023786 hasConcept C173163844 @default.
• W3104023786 hasConcept C2583947 @default.
• W3104023786 hasConcept C2780339063 @default.
• W3104023786 hasConcept C29537977 @default.
• W3104023786 hasConcept C41008148 @default.
• W3104023786 hasConcept C54355233 @default.
• W3104023786 hasConcept C553184892 @default.
• W3104023786 hasConcept C86803240 @default.
• W3104023786 hasConceptScore W3104023786C120665830 @default.
• W3104023786 hasConceptScore W3104023786C121332964 @default.
• W3104023786 hasConceptScore W3104023786C147080431 @default.
• W3104023786 hasConceptScore W3104023786C1491633281 @default.
• W3104023786 hasConceptScore W3104023786C153911025 @default.
• W3104023786 hasConceptScore W3104023786C154945302 @default.
• W3104023786 hasConceptScore W3104023786C160633673 @default.
• W3104023786 hasConceptScore W3104023786C173163844 @default.
• W3104023786 hasConceptScore W3104023786C2583947 @default.
• W3104023786 hasConceptScore W3104023786C2780339063 @default.
• W3104023786 hasConceptScore W3104023786C29537977 @default.
• W3104023786 hasConceptScore W3104023786C41008148 @default.
• W3104023786 hasConceptScore W3104023786C54355233 @default.
• W3104023786 hasConceptScore W3104023786C553184892 @default.
• W3104023786 hasConceptScore W3104023786C86803240 @default.
• W3104023786 hasLocation W31040237861 @default.
• W3104023786 hasOpenAccess W3104023786 @default.
• W3104023786 hasPrimaryLocation W31040237861 @default.
• W3104023786 hasRelatedWork W10107746 @default.
• W3104023786 hasRelatedWork W10920984 @default.
• W3104023786 hasRelatedWork W1121872 @default.
• W3104023786 hasRelatedWork W138378 @default.
• W3104023786 hasRelatedWork W1770923 @default.
• W3104023786 hasRelatedWork W2151077 @default.
• W3104023786 hasRelatedWork W4454899 @default.
• W3104023786 hasRelatedWork W47205 @default.
• W3104023786 hasRelatedWork W6644376 @default.
• W3104023786 hasRelatedWork W8591611 @default.
• W3104023786 isParatext "false" @default.
• W3104023786 isRetracted "false" @default.
• W3104023786 magId "3104023786" @default.
• W3104023786 workType "book-chapter" @default.