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- W3104165890 abstract "Severe asthma is associated with an increased airway smooth muscle (ASM) mass and an altered composition of the extracellular matrix (ECM). Studies have suggested that ECM-ASM cell interactions contribute to this remodeling and its limited reversibility with current therapy. Three-dimensional matrices allow the study of complex cellular responses to different stimuli in an almost natural environment. Our goal was to obtain acellular bronchial matrices and then develop a recellularization protocol with ASM cells. We studied equine bronchi as horses spontaneously develop a human asthma-like disease. The bronchi were decellularized using Triton/Sodium Deoxycholate. The obtained scaffolds retained their anatomical and histological properties. Using immunohistochemistry and a semi-quantitative score to compare native bronchi to scaffolds revealed no significant variation for matrixial proteins. A DNA quantification and electrophoresis revealed that DNA was" @default.
- W3104165890 created "2020-11-23" @default.
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- W3104165890 date "2019-12-26" @default.
- W3104165890 modified "2023-10-06" @default.
- W3104165890 title "Development of a Protocol to Study Bronchial Smooth Muscle Cells Behavior on a Natural Biologic Bronchial Matrix" @default.
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