Matches in SemOpenAlex for { <https://semopenalex.org/work/W3104229816> ?p ?o ?g. }
- W3104229816 abstract "Microbial fatty acids preserve metabolic and environmental information in their hydrogen isotope ratios ( 2 H/ 1 H). This ratio is influenced by parameters that include the 2H/1H of water in the microbial growth environment, and biosynthetic fractionations between water and lipid. In some microbes, this biosynthetic fractionation has been shown to vary systematically with central energy metabolism, and controls on fatty acid 2 H/ 1 H may be linked to the intracellular production of NADPH. We examined the apparent fractionation between media water and the fatty acids produced by Desulfovibrio alaskensis G20. Growth was in batch culture with malate as an electron donor for sulfate respiration, and with pyruvate and fumarate as substrates for fermentation and for sulfate respiration. A larger fractionation was observed as a consequence of respiratory or fermentative growth on pyruvate than growth on fumarate or malate. This difference correlates with opposite apparent flows of electrons through the electron bifurcating/confurcating transhydrogenase NfnAB. When grown on malate or fumarate, mutant strains of D. alaskensis G20 containing transposon disruptions in a copy of nfnAB show different fractionations than the wild type strain. This phenotype is muted during fermentative growth on pyruvate, and it is absent when pyruvate is a substrate for sulfate reduction. All strains and conditions produced similar fatty acid profiles, and the 2 H/ 1 H of individual lipids changed in concert with the mass-weighted average. Unsaturated fatty acids were generally depleted in 2 H relative to their saturated homologues, and anteiso- branched fatty acids were generally depleted in 2 H relative to straight-chain fatty acids. Fractionation correlated with growth rate, a pattern that has also been observed in the fractionation of sulfur isotopes during dissimilatory sulfate reduction by sulfate reducing bacteria." @default.
- W3104229816 created "2020-11-23" @default.
- W3104229816 creator A5035146459 @default.
- W3104229816 creator A5050339093 @default.
- W3104229816 creator A5053784601 @default.
- W3104229816 creator A5072506901 @default.
- W3104229816 date "2016-02-17" @default.
- W3104229816 modified "2023-09-27" @default.
- W3104229816 title "Transhydrogenase and growth substrate influence lipid hydrogen isotope ratios inDesulfovibrio alaskensis G20" @default.
- W3104229816 cites W1485088497 @default.
- W3104229816 cites W150362837 @default.
- W3104229816 cites W1506661817 @default.
- W3104229816 cites W1569137870 @default.
- W3104229816 cites W1904527840 @default.
- W3104229816 cites W1911856927 @default.
- W3104229816 cites W1969073938 @default.
- W3104229816 cites W1970604668 @default.
- W3104229816 cites W1989360756 @default.
- W3104229816 cites W1999607411 @default.
- W3104229816 cites W2002886550 @default.
- W3104229816 cites W2007347287 @default.
- W3104229816 cites W2011766043 @default.
- W3104229816 cites W2019830835 @default.
- W3104229816 cites W2020350008 @default.
- W3104229816 cites W2033880297 @default.
- W3104229816 cites W2047219583 @default.
- W3104229816 cites W2048895821 @default.
- W3104229816 cites W2050802935 @default.
- W3104229816 cites W2051645128 @default.
- W3104229816 cites W2054380694 @default.
- W3104229816 cites W2060617303 @default.
- W3104229816 cites W2063444166 @default.
- W3104229816 cites W2065155050 @default.
- W3104229816 cites W2068064029 @default.
- W3104229816 cites W2087923912 @default.
- W3104229816 cites W2093057648 @default.
- W3104229816 cites W2093133076 @default.
- W3104229816 cites W2096532104 @default.
- W3104229816 cites W2097107919 @default.
- W3104229816 cites W2099876220 @default.
- W3104229816 cites W2101220662 @default.
- W3104229816 cites W2105271117 @default.
- W3104229816 cites W2114520383 @default.
- W3104229816 cites W2115491599 @default.
- W3104229816 cites W2117730614 @default.
- W3104229816 cites W2126388436 @default.
- W3104229816 cites W2132216283 @default.
- W3104229816 cites W2132800014 @default.
- W3104229816 cites W2132926880 @default.
- W3104229816 cites W2139685023 @default.
- W3104229816 cites W2141181239 @default.
- W3104229816 cites W2142428670 @default.
- W3104229816 cites W2148618833 @default.
- W3104229816 cites W2151976860 @default.
- W3104229816 cites W2156498315 @default.
- W3104229816 cites W2157201655 @default.
- W3104229816 cites W2157517587 @default.
- W3104229816 cites W2161184773 @default.
- W3104229816 cites W2161948231 @default.
- W3104229816 cites W2166317358 @default.
- W3104229816 cites W2168696662 @default.
- W3104229816 cites W2170964688 @default.
- W3104229816 cites W2172371128 @default.
- W3104229816 cites W2475400937 @default.
- W3104229816 cites W2477247821 @default.
- W3104229816 cites W2582743722 @default.
- W3104229816 cites W42433123 @default.
- W3104229816 doi "https://doi.org/10.1101/039792" @default.
- W3104229816 hasPublicationYear "2016" @default.
- W3104229816 type Work @default.
- W3104229816 sameAs 3104229816 @default.
- W3104229816 citedByCount "0" @default.
- W3104229816 crossrefType "posted-content" @default.
- W3104229816 hasAuthorship W3104229816A5035146459 @default.
- W3104229816 hasAuthorship W3104229816A5050339093 @default.
- W3104229816 hasAuthorship W3104229816A5053784601 @default.
- W3104229816 hasAuthorship W3104229816A5072506901 @default.
- W3104229816 hasBestOaLocation W31042298161 @default.
- W3104229816 hasConcept C100544194 @default.
- W3104229816 hasConcept C178790620 @default.
- W3104229816 hasConcept C185592680 @default.
- W3104229816 hasConcept C18903297 @default.
- W3104229816 hasConcept C2777107655 @default.
- W3104229816 hasConcept C2777289219 @default.
- W3104229816 hasConcept C2778343803 @default.
- W3104229816 hasConcept C43617362 @default.
- W3104229816 hasConcept C543025807 @default.
- W3104229816 hasConcept C55493867 @default.
- W3104229816 hasConcept C62231903 @default.
- W3104229816 hasConcept C86803240 @default.
- W3104229816 hasConcept C97428945 @default.
- W3104229816 hasConceptScore W3104229816C100544194 @default.
- W3104229816 hasConceptScore W3104229816C178790620 @default.
- W3104229816 hasConceptScore W3104229816C185592680 @default.
- W3104229816 hasConceptScore W3104229816C18903297 @default.
- W3104229816 hasConceptScore W3104229816C2777107655 @default.
- W3104229816 hasConceptScore W3104229816C2777289219 @default.
- W3104229816 hasConceptScore W3104229816C2778343803 @default.
- W3104229816 hasConceptScore W3104229816C43617362 @default.
- W3104229816 hasConceptScore W3104229816C543025807 @default.