Matches in SemOpenAlex for { <https://semopenalex.org/work/W3104262100> ?p ?o ?g. }
- W3104262100 endingPage "2482" @default.
- W3104262100 startingPage "2469" @default.
- W3104262100 abstract "Terahertz waves have attracted great attention in biomolecule research because of the fact that they cover the range of energy levels of weak interactions, skeleton vibrations, and dipole rotations during inter- and intramolecular interactions in biomacromolecules. In this study, we validated the feasibility of employing terahertz time-domain spectroscopy (THz-TDS) for the nondestructive and label-free monitoring of protein digestion. The acid protease, pepsin, was used at its optimal pH to hydrolyze bovine serum albumin. Correspondingly, the control group experiment was also conducted by adjusting the pH value to inactivate pepsin. The progress of these two experiments was tracked by a compact commercial THz-TDS for 1 h. On one hand, the reaction-time-dependent absorption coefficient was calculated, and a direct absorption coefficient analysis was completed. The results indicate that protein hydrolysis can be easily monitored over time by focusing on the variation tendency of the absorption coefficient from a macroscopic perspective. On the other hand, we explored the use of the Debye model to analyze the dielectric properties of the solution during protein hydrolysis. The results of the Debye analysis prove that it is possible to investigate in detail the microscopic dynamics of biomacromolecule solutions at the molecular level by THz-TDS. Our research examined the process of protein hydrolysis by a combination of absorption spectra and Debye analysis and demonstrated that terahertz spectroscopy is a powerful technology for the investigation of biomolecular reactions, with potential applications in variety of fields." @default.
- W3104262100 created "2020-11-23" @default.
- W3104262100 creator A5007091914 @default.
- W3104262100 creator A5018824381 @default.
- W3104262100 creator A5039260364 @default.
- W3104262100 creator A5040599562 @default.
- W3104262100 creator A5058943734 @default.
- W3104262100 creator A5080846142 @default.
- W3104262100 date "2020-12-01" @default.
- W3104262100 modified "2023-10-14" @default.
- W3104262100 title "Terahertz Spectroscopy Tracks Proteolysis by a Joint Analysis of Absorptance and Debye Model" @default.
- W3104262100 cites W1480113445 @default.
- W3104262100 cites W1547655165 @default.
- W3104262100 cites W1572183078 @default.
- W3104262100 cites W1963831440 @default.
- W3104262100 cites W1965639359 @default.
- W3104262100 cites W1965807301 @default.
- W3104262100 cites W1972085077 @default.
- W3104262100 cites W1977893921 @default.
- W3104262100 cites W1978570333 @default.
- W3104262100 cites W1987065580 @default.
- W3104262100 cites W1987457606 @default.
- W3104262100 cites W1994515936 @default.
- W3104262100 cites W2005420127 @default.
- W3104262100 cites W2011983384 @default.
- W3104262100 cites W2012920581 @default.
- W3104262100 cites W2013163441 @default.
- W3104262100 cites W2015249168 @default.
- W3104262100 cites W2025533226 @default.
- W3104262100 cites W2029667874 @default.
- W3104262100 cites W2029937455 @default.
- W3104262100 cites W2033023213 @default.
- W3104262100 cites W2033961192 @default.
- W3104262100 cites W2035854881 @default.
- W3104262100 cites W2035872739 @default.
- W3104262100 cites W2037018413 @default.
- W3104262100 cites W2047393327 @default.
- W3104262100 cites W2057017272 @default.
- W3104262100 cites W2058493027 @default.
- W3104262100 cites W2060270565 @default.
- W3104262100 cites W2061178260 @default.
- W3104262100 cites W2068075383 @default.
- W3104262100 cites W2069586413 @default.
- W3104262100 cites W2070929047 @default.
- W3104262100 cites W2079094292 @default.
- W3104262100 cites W2079249552 @default.
- W3104262100 cites W2082917814 @default.
- W3104262100 cites W2084840523 @default.
- W3104262100 cites W2087050367 @default.
- W3104262100 cites W2125546904 @default.
- W3104262100 cites W2128121055 @default.
- W3104262100 cites W2133081913 @default.
- W3104262100 cites W2139487784 @default.
- W3104262100 cites W2139812504 @default.
- W3104262100 cites W2151044195 @default.
- W3104262100 cites W2156823846 @default.
- W3104262100 cites W2160397925 @default.
- W3104262100 cites W2171267039 @default.
- W3104262100 cites W2177205004 @default.
- W3104262100 cites W2291453359 @default.
- W3104262100 cites W2332846898 @default.
- W3104262100 cites W2344132874 @default.
- W3104262100 cites W2402157049 @default.
- W3104262100 cites W2467109865 @default.
- W3104262100 cites W2564729853 @default.
- W3104262100 cites W2588290812 @default.
- W3104262100 cites W2603772532 @default.
- W3104262100 cites W2610805388 @default.
- W3104262100 cites W2623914537 @default.
- W3104262100 cites W2720962517 @default.
- W3104262100 cites W2755546302 @default.
- W3104262100 cites W2804504128 @default.
- W3104262100 cites W2808412311 @default.
- W3104262100 cites W2905574041 @default.
- W3104262100 cites W2924218006 @default.
- W3104262100 cites W3022434238 @default.
- W3104262100 doi "https://doi.org/10.1016/j.bpj.2020.11.003" @default.
- W3104262100 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7822738" @default.
- W3104262100 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33189688" @default.
- W3104262100 hasPublicationYear "2020" @default.
- W3104262100 type Work @default.
- W3104262100 sameAs 3104262100 @default.
- W3104262100 citedByCount "8" @default.
- W3104262100 countsByYear W31042621002022 @default.
- W3104262100 countsByYear W31042621002023 @default.
- W3104262100 crossrefType "journal-article" @default.
- W3104262100 hasAuthorship W3104262100A5007091914 @default.
- W3104262100 hasAuthorship W3104262100A5018824381 @default.
- W3104262100 hasAuthorship W3104262100A5039260364 @default.
- W3104262100 hasAuthorship W3104262100A5040599562 @default.
- W3104262100 hasAuthorship W3104262100A5058943734 @default.
- W3104262100 hasAuthorship W3104262100A5080846142 @default.
- W3104262100 hasBestOaLocation W31042621001 @default.
- W3104262100 hasConcept C107816215 @default.
- W3104262100 hasConcept C113196181 @default.
- W3104262100 hasConcept C119824511 @default.
- W3104262100 hasConcept C120665830 @default.
- W3104262100 hasConcept C121332964 @default.