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- W3104667664 abstract "Optical tweezers are an invaluable tool for non-contact trapping and micro-manipulation, but their ability to facilitate high-throughput volumetric microrheology of biological samples for mechanobiology research is limited by the precise alignment associated with the excitation and detection of individual bead oscillations. In contrast, radiation pressure from a low numerical aperture optical beam can apply transversely localized force over an extended depth range. We propose photonic force optical coherence elastography (PF-OCE), leveraging phase-sensitive interferometric detection to track sub-nanometre oscillations of beads, embedded in viscoelastic hydrogels, induced by modulated radiation pressure. Since the displacements caused by ultra-low radiation-pressure force are typically obscured by absorption-mediated thermal effects, mechanical responses of the beads were isolated after independent measurement and decoupling of the photothermal response of the hydrogels. Volumetric imaging of bead mechanical responses in hydrogels with different agarose concentrations by PF-OCE was consistent with bulk mechanical characterization of the hydrogels by shear rheometry." @default.
- W3104667664 created "2020-11-23" @default.
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- W3104667664 date "2018-05-25" @default.
- W3104667664 modified "2023-10-17" @default.
- W3104667664 title "Photonic force optical coherence elastography for three-dimensional mechanical microscopy" @default.
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- W3104667664 doi "https://doi.org/10.1038/s41467-018-04357-8" @default.
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