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- W3104809457 abstract "Abstract Extensively drug resistant Acinetobacter baumannii (XDR- Ab ), has emerged as an important pathogen in several outbreaks. The aim of our study was to investigate the eventual genetic relatedness of XDR- Ab strains recovered from burn patients and environment sites in the largest Tunisian Burn Intensive Care Unit (BICU) and to characterize β-lactamase encoding genes in these strains. Between March 04th, 2019 and April 22nd, 2019 an outbreak of XDR- Ab was suspected. Environmental screening was done. All isolates were screened by simplex PCR for β-lactamase genes. Genetic relatedness was determined by pulsed field gel electrophoresis (PFGE) of Apa I-digested total DNA. During the study period, 21 strains of A. baumannii were isolated in burn patients, mainly in blood culture ( n = 7) and central vascular catheter ( n = 6). All strains were susceptible to colistin but resistant to imipenem ( n = 23), ciprofloxacin ( n = 23), amikacin ( n = 22), tigecyclin ( n = 5) and rifampicin ( n = 4). The bla OXA-51-like , bla OXA23, and bla ADC genes were present in all strains. These resistance determinants were associated with bla PER-1 in 10 strains. The IS Aba1 was inserted upstream of bla OXA-23 in all isolates. PFGE revealed two major clusters A ( n = 11) and B ( n = 5). This is the first description in Tunisia of clonally related PER-1 producing XDR- Ab in burn patients with probable environmental origin." @default.
- W3104809457 created "2020-11-23" @default.
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- W3104809457 date "2020-12-17" @default.
- W3104809457 modified "2023-09-27" @default.
- W3104809457 title "Clonal spread of PER-1 and OXA-23 producing extensively drug resistant Acinetobacter baumannii during an outbreak in a burn intensive care unit in Tunisia" @default.
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- W3104809457 doi "https://doi.org/10.1556/030.2020.01208" @default.
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