Matches in SemOpenAlex for { <https://semopenalex.org/work/W3105244906> ?p ?o ?g. }
- W3105244906 endingPage "1308" @default.
- W3105244906 startingPage "1308" @default.
- W3105244906 abstract "Aminotransferases are employed as industrial biocatalysts to produce chiral amines with high enantioselectivity and yield. BpTA-1 and BpTA-2 are the only two pyridoxal 5′-phosphate-dependent fold type IV transaminase enzymes in Bacillus altitudinis W3. Herein, we compared the structures and biochemical characteristics of BpTA-1 and BpTA-2 using bioinformatic analysis, circular dichroism spectroscopy, atomic force microscopy and other approaches. BpTA-1 and BpTA-2 are similar overall; both form homodimers and utilize a catalytic lysine. However, there are distinct differences in the substrate cofactor-binding pocket, molecular weight and the proportion of the secondary structure. Both enzymes have the same stereoselectivity but different enzymatic properties. BpTA-2 is more active under partial alkaline and ambient temperature conditions and BpTA-1 is more sensitive to pH and temperature. BpTA-2 as novel enzyme not only fills the building blocks of transaminase but also has broader industrial application potential for (R)-α-phenethylamines than BpTA-1. Structure-function relationships were explored to assess similarities and differences. The findings lay the foundation for modifying these enzymes via protein engineering to enhance their industrial application potential." @default.
- W3105244906 created "2020-11-23" @default.
- W3105244906 creator A5003685070 @default.
- W3105244906 creator A5019052953 @default.
- W3105244906 creator A5026916310 @default.
- W3105244906 creator A5041269389 @default.
- W3105244906 creator A5057197678 @default.
- W3105244906 creator A5058590640 @default.
- W3105244906 date "2020-11-12" @default.
- W3105244906 modified "2023-09-25" @default.
- W3105244906 title "Structural and Functional Analysis of the Only Two Pyridoxal 5′-Phosphate-Dependent Fold Type IV Transaminases in Bacillus altitudinis W3" @default.
- W3105244906 cites W1599996251 @default.
- W3105244906 cites W1662068511 @default.
- W3105244906 cites W1668340281 @default.
- W3105244906 cites W1926603859 @default.
- W3105244906 cites W1977857718 @default.
- W3105244906 cites W1981215988 @default.
- W3105244906 cites W1986056153 @default.
- W3105244906 cites W1999728411 @default.
- W3105244906 cites W2015779037 @default.
- W3105244906 cites W2025439384 @default.
- W3105244906 cites W2025856735 @default.
- W3105244906 cites W2026727321 @default.
- W3105244906 cites W2031473433 @default.
- W3105244906 cites W2040851590 @default.
- W3105244906 cites W2041571635 @default.
- W3105244906 cites W2041768134 @default.
- W3105244906 cites W2073373061 @default.
- W3105244906 cites W2083715724 @default.
- W3105244906 cites W2096025964 @default.
- W3105244906 cites W2100837269 @default.
- W3105244906 cites W2112047015 @default.
- W3105244906 cites W2118672888 @default.
- W3105244906 cites W2140260269 @default.
- W3105244906 cites W2151522731 @default.
- W3105244906 cites W2158266834 @default.
- W3105244906 cites W2276465174 @default.
- W3105244906 cites W243815175 @default.
- W3105244906 cites W2465627392 @default.
- W3105244906 cites W2524638452 @default.
- W3105244906 cites W2752573573 @default.
- W3105244906 cites W2771222607 @default.
- W3105244906 cites W2789380007 @default.
- W3105244906 cites W2809493191 @default.
- W3105244906 cites W2889118538 @default.
- W3105244906 cites W2894772362 @default.
- W3105244906 cites W2894868033 @default.
- W3105244906 cites W2899847496 @default.
- W3105244906 cites W2940398357 @default.
- W3105244906 cites W2946683133 @default.
- W3105244906 cites W2954163869 @default.
- W3105244906 cites W2967836983 @default.
- W3105244906 cites W2970543113 @default.
- W3105244906 cites W2989696734 @default.
- W3105244906 cites W3002830925 @default.
- W3105244906 cites W3005563213 @default.
- W3105244906 cites W945384332 @default.
- W3105244906 cites W2086995725 @default.
- W3105244906 doi "https://doi.org/10.3390/catal10111308" @default.
- W3105244906 hasPublicationYear "2020" @default.
- W3105244906 type Work @default.
- W3105244906 sameAs 3105244906 @default.
- W3105244906 citedByCount "1" @default.
- W3105244906 countsByYear W31052449062023 @default.
- W3105244906 crossrefType "journal-article" @default.
- W3105244906 hasAuthorship W3105244906A5003685070 @default.
- W3105244906 hasAuthorship W3105244906A5019052953 @default.
- W3105244906 hasAuthorship W3105244906A5026916310 @default.
- W3105244906 hasAuthorship W3105244906A5041269389 @default.
- W3105244906 hasAuthorship W3105244906A5057197678 @default.
- W3105244906 hasAuthorship W3105244906A5058590640 @default.
- W3105244906 hasBestOaLocation W31052449061 @default.
- W3105244906 hasConcept C133571119 @default.
- W3105244906 hasConcept C147816474 @default.
- W3105244906 hasConcept C181199279 @default.
- W3105244906 hasConcept C185592680 @default.
- W3105244906 hasConcept C197957613 @default.
- W3105244906 hasConcept C2776834122 @default.
- W3105244906 hasConcept C2776877979 @default.
- W3105244906 hasConcept C2780859938 @default.
- W3105244906 hasConcept C55493867 @default.
- W3105244906 hasConcept C71240020 @default.
- W3105244906 hasConceptScore W3105244906C133571119 @default.
- W3105244906 hasConceptScore W3105244906C147816474 @default.
- W3105244906 hasConceptScore W3105244906C181199279 @default.
- W3105244906 hasConceptScore W3105244906C185592680 @default.
- W3105244906 hasConceptScore W3105244906C197957613 @default.
- W3105244906 hasConceptScore W3105244906C2776834122 @default.
- W3105244906 hasConceptScore W3105244906C2776877979 @default.
- W3105244906 hasConceptScore W3105244906C2780859938 @default.
- W3105244906 hasConceptScore W3105244906C55493867 @default.
- W3105244906 hasConceptScore W3105244906C71240020 @default.
- W3105244906 hasIssue "11" @default.
- W3105244906 hasLocation W31052449061 @default.
- W3105244906 hasOpenAccess W3105244906 @default.
- W3105244906 hasPrimaryLocation W31052449061 @default.
- W3105244906 hasRelatedWork W13108389 @default.
- W3105244906 hasRelatedWork W1986196461 @default.