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- W3105437584 abstract "Japanese encephalitis virus (JEV) is a member of mosquito-borne flaviviruses. To investigate whether there is a cis-acting genetic element in the coding region of the JEV C protein, which is required for viral replication, we generated four mutants by introducing a various size of deletions in each structural protein-coding region, designated as pJEV/Rep/ΔCC/LUC, pJEV/Rep/ΔC/LUC, pJEV/Rep/ΔprM/LUC, and pJEV/Rep/ΔE/LUC, of these, all replicons except for pJEV/Rep/ΔCC/LUC were competent in replication. Since pJEV/Rep/ΔCC/LUC is the same as pJEV/Rep/ΔC/LUC except for an additional 5′ deletion (nt 132∼201) in the coding region of the C protein, this region appeared to be essential for RNA replication. This is consistent with the proposed cyclization sequence motif in the 5′ region of the C gene, which has been recently shown to be required for replication in other mosquito-borne flaviviruses such as DV, YFV, KUN, and WNV. Thus, our results suggest that a cis-acting genetic element in the coding region of the JEV C protein may play an important role in RNA replication. This study will facilitate the current understanding of JEV RNA replication." @default.
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- W3105437584 date "2006-01-01" @default.
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- W3105437584 title "Evidence for the Presence of acis-acting Element in the Coding Region of the Japanese Encephalitis Virus Capsid Protein" @default.
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- W3105437584 doi "https://doi.org/10.4167/jbv.2006.36.2.125" @default.
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