Matches in SemOpenAlex for { <https://semopenalex.org/work/W3105463310> ?p ?o ?g. }
- W3105463310 endingPage "1269" @default.
- W3105463310 startingPage "1258" @default.
- W3105463310 abstract "We aimed at exploring the function of microRNA-324/cytokine signaling 3 (miR-324/SOCS3) axis in hypoxia/reoxygenation (H/R) -induced cardiomyocyte injury and its underlying mechanism. The differential expression genes were analyzed based on the GSE83500 and GSE48060 datasets from the Gene Expression Omnibus (GEO) database. Then, to conduct the function enrichment analysis, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases were used. The upstream regulatory microRNAs (miRNAs) of the identified genes were predicted by miRanda, miRWalk, and TargetScan websites. MiR-324 expression was measured with quantitative real-time polymerase chain reaction (qRT-PCR). The target binding of miR-324 and SOCS3 was established by dual-luciferase reporter assay. Cardiomyocyte proliferation was analyzed by cell counting kit-8 (CCK-8) assay, whereas the apoptosis was investigated via flow cytometry. The expression of TNF pathway-related proteins was detected by western blot analysis. SOCS3 was upregulated in patients with myocardial infarction (MI), and function enrichment analyses proved that SOCS3 was enriched in TNF signaling pathway. Moreover, we found that miR-324 was the upstream regulatory miRNA of SOCS3 and negatively regulated SOCS3 expression. MiR-324 was downregulated in cardiomyocytes with H/R-induced injury, inhibiting cell proliferation. In the H/R model, SOCS3 suppresses cardiomyocyte proliferation, which was recovered by miR-324, and induces cell apoptosis, which was repressed by miR-324 via regulating the expression of cleaved caspase-3 and p P38-MAPK. MiR-324 upregulation decreased the protein levels of TNF-α, p-P65, and p-IκBα in cardiomyocytes that suffered from H/R, which was reversed with SOCS3 overexpression. MiR-324/SOCS3 axis could improve the H/R-induced injury of cardiomyocytes via regulating TNF/NF-κB signaling pathway, and this might provide a new therapy strategy for myocardial ischemia." @default.
- W3105463310 created "2020-11-23" @default.
- W3105463310 creator A5008171524 @default.
- W3105463310 creator A5009352908 @default.
- W3105463310 creator A5013395751 @default.
- W3105463310 creator A5037369499 @default.
- W3105463310 creator A5059187451 @default.
- W3105463310 creator A5071537403 @default.
- W3105463310 date "2020-11-28" @default.
- W3105463310 modified "2023-09-29" @default.
- W3105463310 title "MiR-324/SOCS3 Axis Protects Against Hypoxia/Reoxygenation-Induced Cardiomyocyte Injury and Regulates Myocardial Ischemia via TNF/NF-κB Signaling Pathway" @default.
- W3105463310 cites W1481725997 @default.
- W3105463310 cites W1545911557 @default.
- W3105463310 cites W1669479280 @default.
- W3105463310 cites W1981694995 @default.
- W3105463310 cites W1986060457 @default.
- W3105463310 cites W1986207978 @default.
- W3105463310 cites W1987248329 @default.
- W3105463310 cites W1988502798 @default.
- W3105463310 cites W1989358807 @default.
- W3105463310 cites W1991865594 @default.
- W3105463310 cites W1998457769 @default.
- W3105463310 cites W2013894061 @default.
- W3105463310 cites W2016986936 @default.
- W3105463310 cites W2020428280 @default.
- W3105463310 cites W2028644104 @default.
- W3105463310 cites W2036399835 @default.
- W3105463310 cites W2036632357 @default.
- W3105463310 cites W2048362474 @default.
- W3105463310 cites W2050720508 @default.
- W3105463310 cites W2051115286 @default.
- W3105463310 cites W2053595149 @default.
- W3105463310 cites W2059062907 @default.
- W3105463310 cites W2060339708 @default.
- W3105463310 cites W2066138632 @default.
- W3105463310 cites W2066279279 @default.
- W3105463310 cites W2080999576 @default.
- W3105463310 cites W2086287235 @default.
- W3105463310 cites W2092013338 @default.
- W3105463310 cites W2101986300 @default.
- W3105463310 cites W2116380600 @default.
- W3105463310 cites W2129960497 @default.
- W3105463310 cites W2137730290 @default.
- W3105463310 cites W2140114458 @default.
- W3105463310 cites W2146272458 @default.
- W3105463310 cites W2149161770 @default.
- W3105463310 cites W2152622419 @default.
- W3105463310 cites W2159974171 @default.
- W3105463310 cites W2168645863 @default.
- W3105463310 cites W2272962241 @default.
- W3105463310 cites W2317539243 @default.
- W3105463310 cites W2333876020 @default.
- W3105463310 cites W2528296560 @default.
- W3105463310 cites W2530096609 @default.
- W3105463310 cites W2599749400 @default.
- W3105463310 cites W2624392106 @default.
- W3105463310 cites W2674744780 @default.
- W3105463310 cites W2745407714 @default.
- W3105463310 cites W2765588508 @default.
- W3105463310 cites W2766665573 @default.
- W3105463310 cites W2769628390 @default.
- W3105463310 cites W2769710939 @default.
- W3105463310 cites W2782612303 @default.
- W3105463310 cites W2800345260 @default.
- W3105463310 cites W2800524871 @default.
- W3105463310 cites W2871281777 @default.
- W3105463310 cites W2895354228 @default.
- W3105463310 cites W2895390950 @default.
- W3105463310 cites W2898336829 @default.
- W3105463310 cites W2898979195 @default.
- W3105463310 cites W2900999162 @default.
- W3105463310 cites W2901130924 @default.
- W3105463310 cites W2909380704 @default.
- W3105463310 cites W2913765920 @default.
- W3105463310 cites W2913989805 @default.
- W3105463310 cites W2918557729 @default.
- W3105463310 cites W2935928027 @default.
- W3105463310 cites W2944692499 @default.
- W3105463310 cites W2950793396 @default.
- W3105463310 cites W2953205469 @default.
- W3105463310 cites W3121415533 @default.
- W3105463310 cites W391098122 @default.
- W3105463310 doi "https://doi.org/10.1536/ihj.19-687" @default.
- W3105463310 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33191336" @default.
- W3105463310 hasPublicationYear "2020" @default.
- W3105463310 type Work @default.
- W3105463310 sameAs 3105463310 @default.
- W3105463310 citedByCount "10" @default.
- W3105463310 countsByYear W31054633102021 @default.
- W3105463310 countsByYear W31054633102022 @default.
- W3105463310 countsByYear W31054633102023 @default.
- W3105463310 crossrefType "journal-article" @default.
- W3105463310 hasAuthorship W3105463310A5008171524 @default.
- W3105463310 hasAuthorship W3105463310A5009352908 @default.
- W3105463310 hasAuthorship W3105463310A5013395751 @default.
- W3105463310 hasAuthorship W3105463310A5037369499 @default.
- W3105463310 hasAuthorship W3105463310A5059187451 @default.
- W3105463310 hasAuthorship W3105463310A5071537403 @default.