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- W3105490435 abstract "Abstract In order to better understand the Fusarium head blight disease, reliable real-time PCR assays for detection and quantification of fungal species belonging to the Fusarium and Microdochium genus are needed. Specific qPCR assays were developed for nine of those species. All criteria required for reproducing the assays are presented. The assays were species specific and allow quantification of at least 5 pg of fungal DNA and detection of 0.5 pg of fungal DNA per PCR reaction. Moreover we showed that the quantification performances of the tests were not altered in the presence of DNA of closely related species in the sample. The assays were tested on field samples and have been already used in greenhouse experiments." @default.
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- W3105490435 date "2018-02-27" @default.
- W3105490435 modified "2023-10-15" @default.
- W3105490435 title "Optimized real time QPCR assays for detection and quantification of Fusarium and Microdochium species involved in wheat head blight as defined by MIQE guidelines" @default.
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- W3105490435 doi "https://doi.org/10.1101/272534" @default.
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