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- W3105786320 abstract "Fc-gamma receptor (FcγR) activation by soluble IgG immune complexes (sICs) is thought to be a major mechanism of inflammation in certain autoimmune diseases such as systemic lupus erythematosus (SLE). A robust and scalable test system allowing for the detection and quantification of sIC bioactivity is missing. Therefore, we developed a comprehensive reporter cell panel capable of measuring the sIC-mediated activation of individual human and mouse FcγRs. We show that compared to human FcγRs IIB and III, human FcγRs I and IIA lack sensitivity to sICs. Further, the assay proved to be sensitive to sIC stoichiometry and size enabling us to demonstrate for the first time a complete translation of the Heidelberger-Kendall precipitation curve to FcγR responsiveness. This was validated using primary immune cells. The approach was applied to quantify sIC-mediated FcγR activation using sera from SLE patients or from mouse models of lupus and arthritis. Thus, in clinical practice, it might be employed as a toolbox enabling the evaluation of FcγR activation by sICs as a biomarker for disease activity in immune-complex mediated diseases." @default.
- W3105786320 created "2020-11-23" @default.
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- W3105786320 date "2020-11-11" @default.
- W3105786320 modified "2023-09-23" @default.
- W3105786320 title "Immune complex solubility and size govern Fc-gamma receptor responses: A scalable cell-based reporter system" @default.
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