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- W3106284032 abstract "Abstract Activated Ezrin-Radixin-Moesin (ERM) proteins link the plasma membrane to the actin cytoskeleton to generate apical structures, including microvilli. Among many kinases implicated in ERM activation are the homologs LOK and SLK. CRISPR/Cas9 was used to knockout all ERM proteins or LOK/SLK in human cells. LOK/SLK knockout eliminates all ERM activating phosphorylation. The apical domain of cells lacking LOK/SLK or ERMs is strikingly similar and selectively altered, with loss of microvill, and junctional actin replaced by ectopic myosin-II containing apical stress-fiber-like structures. Constitutively active ezrin can reverse the phenotypes of either ERMs or LOK/SLK knockouts, showing that the major function of LOK/SLK is to activate ERMs. Both knockout lines have elevated active RhoA with concomitant enhanced myosin light chain phosphorylation, revealing that active ERMs are negative regulators of RhoA. As RhoA-GTP activates LOK/SLK to activate ERM proteins, the ability of active ERMs to negatively regulate RhoA-GTP represents a novel local feedback loop necessary for the proper apical morphology of epithelial cells." @default.
- W3106284032 created "2020-11-23" @default.
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- W3106284032 date "2020-07-02" @default.
- W3106284032 modified "2023-09-26" @default.
- W3106284032 title "RhoA effectors LOK/SLK activate ERM proteins to locally inhibit RhoA and define apical morphology" @default.
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- W3106284032 doi "https://doi.org/10.1101/2020.07.02.185298" @default.
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