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- W3106404481 abstract "Introduction: Cardiac arrest (CA) causes ischemia and reperfusion damage to the entire body. Augmented expression of circulating microRNA (miR)-122 has been associated with poor neurological outcome after CA, as well as drug and alcohol-induced liver injury in human. Plasma exosomal miR-300 has shown to be up regulated in transient ischemic attack while cellular miR-300 expression increased after traumatic brain injury in rodent model. Hypothesis: The response of tissue injury can be determined by measuring differential expression of miR. This study sought to quantify two specific miR-122 and 300, from the intracellular compartment of the brain, kidney, and liver tissues to determine the injury pattern after severe CA and resuscitation. Methods: Male Sprague-Dawley rats were assigned into 2 groups: control (no CA or CA and resuscitation) and 20 min CA followed by 30 min cardiopulmonary bypass (CPB) resuscitation (n=3). Brain, kidney, and liver tissues were isolated and homogenized. Total RNA was isolated from homogenized tissue by trizol method, RNA was then converted to cDNA by RT kit and processed for miR-122 and miR-300 quantification using quantitative real-time polymerase chain reaction (qRT-PCR). RNU6 was used as an endogenous control and results were expressed in fold change. Results: Brain after CA and resuscitation revealed an increased trend in expression of miR-122 (p=0.053) and miR-300 (p=0.57). Liver also demonstrated an increasing trend in miR-122 (p=0.15) and miR-300 (p=0.007) expression. The increasing trend of miR-122 and miR-300 expression in brain and liver following CA and resuscitation suggests reperfusion injury in the early phase of resuscitation. Kidney demonstrated a lower expression of both miR-122 and miR-300 after CA and resuscitation, which suggests that the event of CA and resuscitation further down regulate miR-122 and miR-300 expression. Conclusion: Levels of intracellular miR-122 and 300 in the brain, kidney, and liver appear to be altered after CA and resuscitation. Although, only one of the miR showed a significant difference in our small exploratory study, the current finding suggests that tissue specific miR levels may provide insight about specific injury following CA." @default.
- W3106404481 created "2020-11-23" @default.
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- W3106404481 date "2020-11-17" @default.
- W3106404481 modified "2023-09-23" @default.
- W3106404481 title "Abstract 208: MicroRNA 122 and 300 as a Potential Prognosticator of Brain, Kidney and Liver Damage in Response to Ischemic-reperfusion Injury After Severe Rodent Cardiac Arrest" @default.
- W3106404481 doi "https://doi.org/10.1161/circ.142.suppl_4.208" @default.
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