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- W3106491566 abstract "Experiments in cell cultures have been useful for investigating a number of RNA endonucleases. However, endonuclease decay intermediates are often challenging to study in cellulo, as decay intermediates are rapidly degraded by exoribonucleases. Thus, cell-free assays have been critical for assessing endonuclease kinetics. Here, we describe such an in vitro assay to analyze endoribonuclease activity using recombinant proteins and end-radiolabeled RNA oligonucleotides. Specifically, we detail a protocol for assaying the endoribonuclease activity and kinetics of the human MARF1 protein." @default.
- W3106491566 created "2020-11-23" @default.
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- W3106491566 date "2020-11-18" @default.
- W3106491566 modified "2023-09-23" @default.
- W3106491566 title "A Cell-Free System for Investigating Human MARF1 Endonuclease Activity" @default.
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- W3106491566 doi "https://doi.org/10.1007/978-1-0716-0935-4_21" @default.
- W3106491566 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33201479" @default.
- W3106491566 hasPublicationYear "2020" @default.
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