FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3106631681> ?p ?o ?g. }

• W3106631681 endingPage "101130" @default.
• W3106631681 startingPage "101130" @default.
• W3106631681 abstract "Brown adipose tissue (BAT) is specialized in thermogenesis. The conversion of energy into heat in brown adipocytes proceeds via stimulation of β-adrenergic receptor (βAR)-dependent signaling and activation of mitochondrial uncoupling protein 1 (UCP1). We have previously demonstrated a functional role for pannexin-1 (Panx1) channels in white adipose tissue; however, it is not known whether Panx1 channels play a role in the regulation of brown adipocyte function. Here, we tested the hypothesis that Panx1 channels are involved in brown adipocyte activation and thermogenesis. In an immortalized brown pre-adipocytes cell line, Panx1 currents were measured using patch-clamp electrophysiology. Flow cytometry was used for assessment of dye uptake and luminescence assays for adenosine triphosphate (ATP) release, and cellular temperature measurement was performed using a ratiometric fluorescence thermometer. We used RNA interference and expression plasmids to manipulate expression of wild-type and mutant Panx1. We used previously described adipocyte-specific Panx1 knockout mice (Panx1Adip-/-) and generated brown adipocyte-specific Panx1 knockout mice (Panx1BAT-/-) to study pharmacological or cold-induced thermogenesis. Glucose uptake into brown adipose tissue was quantified by positron emission tomography (PET) analysis of 18F-fluorodeoxyglucose (18F-FDG) content. BAT temperature was measured using an implantable telemetric temperature probe. In brown adipocytes, Panx1 channel activity was induced either by apoptosis-dependent caspase activation or by β3AR stimulation via a novel mechanism that involves Gβγ subunit binding to Panx1. Inactivation of Panx1 channels in cultured brown adipocytes resulted in inhibition of β3AR-induced lipolysis, UCP-1 expression, and cellular thermogenesis. In mice, adiponectin-Cre-dependent genetic deletion of Panx1 in all adipose tissue depots resulted in defective β3AR agonist- or cold-induced thermogenesis in BAT and suppressed beigeing of white adipose tissue. UCP1-Cre-dependent Panx1 deletion specifically in brown adipocytes reduced the capacity for adaptive thermogenesis without affecting beigeing of white adipose tissue and aggravated diet-induced obesity and insulin resistance. These data demonstrate that Gβγ-dependent Panx1 channel activation is involved in β3AR-induced thermogenic regulation in brown adipocytes. Identification of Panx1 channels in BAT as novel thermo-regulatory elements downstream of β3AR activation may have therapeutic implications." @default.
• W3106631681 created "2020-12-07" @default.
• W3106631681 creator A5000159326 @default.
• W3106631681 creator A5017544749 @default.
• W3106631681 creator A5021535519 @default.
• W3106631681 creator A5026140682 @default.
• W3106631681 creator A5030081677 @default.
• W3106631681 creator A5033870596 @default.
• W3106631681 creator A5034480104 @default.
• W3106631681 creator A5035655436 @default.
• W3106631681 creator A5039339384 @default.
• W3106631681 creator A5043171743 @default.
• W3106631681 creator A5048577226 @default.
• W3106631681 creator A5050327070 @default.
• W3106631681 creator A5051208927 @default.
• W3106631681 creator A5055086428 @default.
• W3106631681 creator A5060349140 @default.
• W3106631681 creator A5063630024 @default.
• W3106631681 creator A5064145098 @default.
• W3106631681 creator A5066022617 @default.
• W3106631681 creator A5068275315 @default.
• W3106631681 creator A5076239980 @default.
• W3106631681 creator A5080986969 @default.
• W3106631681 date "2021-02-01" @default.
• W3106631681 modified "2023-10-16" @default.
• W3106631681 title "Adaptive thermogenesis in brown adipose tissue involves activation of pannexin-1 channels" @default.
• W3106631681 cites W1535117441 @default.
• W3106631681 cites W1761885863 @default.
• W3106631681 cites W1965447971 @default.
• W3106631681 cites W1968522305 @default.
• W3106631681 cites W1970656757 @default.
• W3106631681 cites W1970812735 @default.
• W3106631681 cites W1970896416 @default.
• W3106631681 cites W1971084572 @default.
• W3106631681 cites W1972836378 @default.
• W3106631681 cites W1978593376 @default.
• W3106631681 cites W1981706410 @default.
• W3106631681 cites W1981909596 @default.
• W3106631681 cites W1989567728 @default.
• W3106631681 cites W1990577987 @default.
• W3106631681 cites W1999673165 @default.
• W3106631681 cites W2008561463 @default.
• W3106631681 cites W2017612052 @default.
• W3106631681 cites W2019100110 @default.
• W3106631681 cites W2019615270 @default.
• W3106631681 cites W2025259609 @default.
• W3106631681 cites W2027589498 @default.
• W3106631681 cites W2029913563 @default.
• W3106631681 cites W2040478551 @default.
• W3106631681 cites W2061777482 @default.
• W3106631681 cites W2085780740 @default.
• W3106631681 cites W2087825813 @default.
• W3106631681 cites W2098457930 @default.
• W3106631681 cites W2101235551 @default.
• W3106631681 cites W2102246235 @default.
• W3106631681 cites W2106701169 @default.
• W3106631681 cites W2115313813 @default.
• W3106631681 cites W2116927194 @default.
• W3106631681 cites W2122275827 @default.
• W3106631681 cites W2123367576 @default.
• W3106631681 cites W2127653983 @default.
• W3106631681 cites W2128291544 @default.
• W3106631681 cites W2132358997 @default.
• W3106631681 cites W2135941843 @default.
• W3106631681 cites W2150612042 @default.
• W3106631681 cites W2160146943 @default.
• W3106631681 cites W2164410780 @default.
• W3106631681 cites W2337936516 @default.
• W3106631681 cites W2542636225 @default.
• W3106631681 cites W2582947238 @default.
• W3106631681 cites W2742463962 @default.
• W3106631681 cites W2752817656 @default.
• W3106631681 cites W2763536199 @default.
• W3106631681 cites W2764017545 @default.
• W3106631681 cites W2767407105 @default.
• W3106631681 cites W2799659527 @default.
• W3106631681 cites W2883879320 @default.
• W3106631681 cites W2898294884 @default.
• W3106631681 cites W2917898015 @default.
• W3106631681 cites W2940254466 @default.
• W3106631681 cites W2945045299 @default.
• W3106631681 cites W2968055927 @default.
• W3106631681 cites W2996797552 @default.
• W3106631681 cites W3001317202 @default.
• W3106631681 cites W3005705986 @default.
• W3106631681 cites W3010985052 @default.
• W3106631681 cites W3032333420 @default.
• W3106631681 doi "https://doi.org/10.1016/j.molmet.2020.101130" @default.
• W3106631681 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7779784" @default.
• W3106631681 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33248294" @default.
• W3106631681 hasPublicationYear "2021" @default.
• W3106631681 type Work @default.
• W3106631681 sameAs 3106631681 @default.
• W3106631681 citedByCount "17" @default.
• W3106631681 countsByYear W31066316812021 @default.
• W3106631681 countsByYear W31066316812022 @default.
• W3106631681 countsByYear W31066316812023 @default.
• W3106631681 crossrefType "journal-article" @default.

• next