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- W3106730531 abstract "Abstract In the search for anti-renal autoreactivity in human lupus nephritis, we stimulated blood-derived CD4 + T cells from patients with systemic lupus erythematosus with various kidney lysates. Although only minor responses were detectable, these experiments led to the development of a search algorithm that combined autoantibody association with human lupus nephritis and target gene expression in inflamed kidneys. Applying this algorithm, five potential T cell antigens were identified. Blood-derived CD4 + T cells were then stimulated with these antigens. The cells were magnetically enriched prior to measurement with flow cytometry to facilitate the detection of very rare autoantigen-specific cells. The detected responses were dominated by IFN-γ-producing CD4 + T cells. Additionally, IL-10-producing CD4 + T cells were found. In a next step, T cell reactivity to each single antigen was independently evaluated with T cell libraries and [ 3 H]-thymidine incorporation assays. Here, Vimentin and Annexin A2 were identified as the main T cell targets. Finally, Vimentin reactive T cells were also found in the urine of three patients with active disease. Overall, our experiments show that antigen-specific CD4 + T cells targeting renally expressed antigens arise in human lupus nephritis and correlate with disease activity and are mainly of the Th1 subset." @default.
- W3106730531 created "2020-12-07" @default.
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- W3106730531 date "2020-12-04" @default.
- W3106730531 modified "2023-10-16" @default.
- W3106730531 title "Identification and characterization of antigen-specific CD4+ T cells targeting renally expressed antigens in human lupus nephritis with two independent methods" @default.
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- W3106730531 doi "https://doi.org/10.1038/s41598-020-78223-3" @default.
- W3106730531 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7718878" @default.
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- W3106730531 hasPublicationYear "2020" @default.
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