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• W3106894959 endingPage "250" @default.
• W3106894959 startingPage "237" @default.
• W3106894959 abstract "Hepcidin, a 25-amino acid peptide encoded by the HAMP gene and produced mainly by hepatocytes and macrophages, is a mediator of innate immunity and the central iron-regulatory hormone. Circulating hepcidin controls iron efflux by inducing degradation of the cellular iron exporter ferroportin. HCV infection is associated with hepatic iron overload and elevated serum iron, which correlate with poor antiviral responses. The HCV nonstructural NS5A protein is known to function in multiple aspects of the HCV life cycle, probably exerting its activity in concert with cellular factor(s). In this study, we attempted to delineate the effect of HCV NS5A on HAMP gene expression. We observed that transient transfection of hepatoma cell lines with HCV NS5A resulted in down-regulation of HAMP promoter activity. A similar effect was evident after transduction of Huh7 cells with a recombinant baculovirus vector expressing NS5A protein. We proceeded to construct an NS5A-expressing stable cell line, which also exhibited down-regulation of HAMP gene promoter activity and significant reduction of HAMP mRNA and hepcidin protein levels. Concurrent expression of HCV core protein, a well-characterized hepcidin inducer, revealed antagonism between those two proteins for hepcidin regulation. In attempting to identify the pathways involved in NS5A-driven reduction of hepcidin levels, we ruled out any NS5A-induced alterations in the expression of the well-known hepcidin inducers SMAD4 and STAT3. Further analysis linked the abundance of intracellular zinc ions and the deregulation of the MTF-1/MRE/hepcidin axis with the observed phenomenon. This effect could be associated with distinct phases in HCV life cycle." @default.
• W3106894959 created "2020-12-07" @default.
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• W3106894959 date "2020-12-13" @default.
• W3106894959 modified "2023-10-12" @default.
• W3106894959 title "The Hepatitis C virus NS5A and core proteins exert antagonistic effects on HAMP gene expression: the hidden interplay with the MTF‐1/MRE pathway" @default.
• W3106894959 cites W1003598251 @default.
• W3106894959 cites W1495909545 @default.
• W3106894959 cites W1901626724 @default.
• W3106894959 cites W1968603331 @default.
• W3106894959 cites W1970739518 @default.
• W3106894959 cites W1971884054 @default.
• W3106894959 cites W1973509755 @default.
• W3106894959 cites W1974980105 @default.
• W3106894959 cites W1975922979 @default.
• W3106894959 cites W1980042430 @default.
• W3106894959 cites W1984515680 @default.
• W3106894959 cites W1986567995 @default.
• W3106894959 cites W1989910341 @default.
• W3106894959 cites W1990733264 @default.
• W3106894959 cites W1995278959 @default.
• W3106894959 cites W1998872615 @default.
• W3106894959 cites W2001563512 @default.
• W3106894959 cites W2007440356 @default.
• W3106894959 cites W2012808888 @default.
• W3106894959 cites W2012929232 @default.
• W3106894959 cites W2015248217 @default.
• W3106894959 cites W2020550910 @default.
• W3106894959 cites W2022869504 @default.
• W3106894959 cites W2033125798 @default.
• W3106894959 cites W2039007136 @default.
• W3106894959 cites W2043928258 @default.
• W3106894959 cites W2047508872 @default.
• W3106894959 cites W2050833520 @default.
• W3106894959 cites W2054421541 @default.
• W3106894959 cites W2057967679 @default.
• W3106894959 cites W2059144268 @default.
• W3106894959 cites W2060061109 @default.
• W3106894959 cites W2061122330 @default.
• W3106894959 cites W2061565132 @default.
• W3106894959 cites W2064883199 @default.
• W3106894959 cites W2065010611 @default.
• W3106894959 cites W2065585203 @default.
• W3106894959 cites W2068313428 @default.
• W3106894959 cites W2068382047 @default.
• W3106894959 cites W2069594569 @default.
• W3106894959 cites W2070968956 @default.
• W3106894959 cites W2073675795 @default.
• W3106894959 cites W2076000238 @default.
• W3106894959 cites W2077230125 @default.
• W3106894959 cites W2077890484 @default.
• W3106894959 cites W2081147927 @default.
• W3106894959 cites W2082026289 @default.
• W3106894959 cites W2082933842 @default.
• W3106894959 cites W2083282915 @default.
• W3106894959 cites W2088185132 @default.
• W3106894959 cites W2090540258 @default.
• W3106894959 cites W2090825777 @default.
• W3106894959 cites W2093214540 @default.
• W3106894959 cites W2096604412 @default.
• W3106894959 cites W2103263770 @default.
• W3106894959 cites W2113445958 @default.
• W3106894959 cites W2115610036 @default.
• W3106894959 cites W2116374020 @default.
• W3106894959 cites W2125422626 @default.
• W3106894959 cites W2125721076 @default.
• W3106894959 cites W2144347299 @default.
• W3106894959 cites W2146729562 @default.
• W3106894959 cites W2150926825 @default.
• W3106894959 cites W2152977212 @default.
• W3106894959 cites W2164984156 @default.
• W3106894959 cites W2167499479 @default.
• W3106894959 cites W2172197126 @default.
• W3106894959 cites W2218944944 @default.
• W3106894959 cites W2231278137 @default.
• W3106894959 cites W2320908715 @default.
• W3106894959 cites W2757036630 @default.
• W3106894959 cites W3043839133 @default.
• W3106894959 cites W8890124 @default.
• W3106894959 cites W1982057459 @default.
• W3106894959 doi "https://doi.org/10.1002/2211-5463.13048" @default.
• W3106894959 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7780115" @default.
• W3106894959 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33247551" @default.
• W3106894959 hasPublicationYear "2020" @default.
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