Matches in SemOpenAlex for { <https://semopenalex.org/work/W3106992092> ?p ?o ?g. }
- W3106992092 abstract "Summary The trillions of cells that constitute the human body are developed from a fertilized egg through embryogenesis. However, cellular dynamics and developmental outcomes of embryonic cells in humans remain to be largely unknown due to the technical and ethical challenges. Here, we explored whole-genomes of 334 single-cell expanded clones and targeted deep-sequences of 379 bulk tissues obtained from various anatomical locations from seven individuals. Using the discovered 1,688,652 somatic mutations as an intrinsic barcode, we reconstructed cellular phylogenetic trees that provide novel insights into early human embryogenesis. Our findings suggest (1) endogenous mutational rate that is higher in the first cell division of life but decreases to ~1 per cell per cell division later in life, (2) universal unequal contribution of early cells into embryo proper resulting from early cellular bottlenecks that stochastically separate epiblasts from embryonic cells (3) uneven differential outcomes of early cells into three germ layers, left-right and cranio-caudal tissues, (4) emergence of a few ancestral cells that will contribute to the substantial fraction of adult blood cells, and (5) presence of mitochondrial DNA heteroplasmy in the fertilized egg. Our approach additionally provides insights into the age-related mutational processes including UV-mediated mutagenesis and loss of chromosome X or Y in normal somatic cells. Taken together, this study scrutinized somatic mosaicism, clonal architecture, and cellular dynamics in human embryogenesis at an unprecedented level and provides a foundation for future studies to complete cellular phylogenies in human embryogenesis." @default.
- W3106992092 created "2020-12-07" @default.
- W3106992092 creator A5004445429 @default.
- W3106992092 creator A5005650192 @default.
- W3106992092 creator A5008807591 @default.
- W3106992092 creator A5028688587 @default.
- W3106992092 creator A5028785394 @default.
- W3106992092 creator A5029773293 @default.
- W3106992092 creator A5030341663 @default.
- W3106992092 creator A5030458579 @default.
- W3106992092 creator A5037123236 @default.
- W3106992092 creator A5042703505 @default.
- W3106992092 creator A5046809047 @default.
- W3106992092 creator A5049364024 @default.
- W3106992092 creator A5050866517 @default.
- W3106992092 creator A5052179421 @default.
- W3106992092 creator A5055647121 @default.
- W3106992092 creator A5059131847 @default.
- W3106992092 creator A5069177443 @default.
- W3106992092 creator A5072548045 @default.
- W3106992092 creator A5073453541 @default.
- W3106992092 creator A5080087633 @default.
- W3106992092 creator A5085660248 @default.
- W3106992092 creator A5086268762 @default.
- W3106992092 date "2020-11-24" @default.
- W3106992092 modified "2023-09-27" @default.
- W3106992092 title "Clonal dynamics in early human embryogenesis inferred from somatic mutation" @default.
- W3106992092 cites W1509720837 @default.
- W3106992092 cites W1534550228 @default.
- W3106992092 cites W1934389460 @default.
- W3106992092 cites W1956871134 @default.
- W3106992092 cites W1986006829 @default.
- W3106992092 cites W1989952069 @default.
- W3106992092 cites W1990385569 @default.
- W3106992092 cites W1994561301 @default.
- W3106992092 cites W1996048984 @default.
- W3106992092 cites W2008520570 @default.
- W3106992092 cites W2011116207 @default.
- W3106992092 cites W2012785339 @default.
- W3106992092 cites W2021341670 @default.
- W3106992092 cites W2028251374 @default.
- W3106992092 cites W2040151712 @default.
- W3106992092 cites W2042610635 @default.
- W3106992092 cites W2042734644 @default.
- W3106992092 cites W2048558997 @default.
- W3106992092 cites W2056298328 @default.
- W3106992092 cites W2059300459 @default.
- W3106992092 cites W2062691001 @default.
- W3106992092 cites W2069512527 @default.
- W3106992092 cites W2074507113 @default.
- W3106992092 cites W2085947407 @default.
- W3106992092 cites W2103441770 @default.
- W3106992092 cites W2106619117 @default.
- W3106992092 cites W2109991075 @default.
- W3106992092 cites W2119180969 @default.
- W3106992092 cites W2125336256 @default.
- W3106992092 cites W2128718933 @default.
- W3106992092 cites W2129136620 @default.
- W3106992092 cites W2136336930 @default.
- W3106992092 cites W2146005104 @default.
- W3106992092 cites W2150575159 @default.
- W3106992092 cites W2150603502 @default.
- W3106992092 cites W2152061559 @default.
- W3106992092 cites W2155943701 @default.
- W3106992092 cites W2165974744 @default.
- W3106992092 cites W2166664098 @default.
- W3106992092 cites W2171892226 @default.
- W3106992092 cites W2218943248 @default.
- W3106992092 cites W2219142985 @default.
- W3106992092 cites W2411874306 @default.
- W3106992092 cites W2513727910 @default.
- W3106992092 cites W2517993048 @default.
- W3106992092 cites W2547187744 @default.
- W3106992092 cites W2603764892 @default.
- W3106992092 cites W2611387862 @default.
- W3106992092 cites W2770273110 @default.
- W3106992092 cites W2774274736 @default.
- W3106992092 cites W2774506962 @default.
- W3106992092 cites W2790816122 @default.
- W3106992092 cites W2883177040 @default.
- W3106992092 cites W2885069342 @default.
- W3106992092 cites W2889734305 @default.
- W3106992092 cites W2897064819 @default.
- W3106992092 cites W2898789672 @default.
- W3106992092 cites W2902846198 @default.
- W3106992092 cites W2905550051 @default.
- W3106992092 cites W2905667684 @default.
- W3106992092 cites W2929496863 @default.
- W3106992092 cites W2947095040 @default.
- W3106992092 cites W2952564192 @default.
- W3106992092 cites W2980068720 @default.
- W3106992092 cites W2981329637 @default.
- W3106992092 cites W2981552021 @default.
- W3106992092 cites W2981923411 @default.
- W3106992092 cites W2990327991 @default.
- W3106992092 cites W2991701263 @default.
- W3106992092 cites W2996524386 @default.
- W3106992092 cites W3003949123 @default.
- W3106992092 cites W3004480399 @default.
- W3106992092 cites W3006500278 @default.