Matches in SemOpenAlex for { <https://semopenalex.org/work/W3107097029> ?p ?o ?g. }
- W3107097029 abstract "Antimicrobial peptides (AMPs) play pivotal roles in protecting against microbial infection in fish. However, AMPs from topmouth culter ( Erythroculter ilishaeformis ) are rarely known. In our study, we isolated an AMP from the head kidney of topmouth culter, which belonged to liver-expressed antimicrobial peptide 2 (LEAP-2) family. Topmouth culter LEAP-2 showed inhibitory effects on aquatic bacterial growth, including antibiotic-resistant bacteria, with minimal inhibitory concentration values ranging from 18.75 to 150 μg/ml. It was lethal for Aeromonas hydrophila (resistant to ampicillin), and took less than 60 min to kill A. hydrophila at a concentration of 5 × MIC. Scanning electron microscope (SEM) and SYTOX Green uptake assay indicated that it impaired the integrity of bacterial membrane by eliciting pore formation, thereby increasing the permeabilization of bacterial membrane. In addition, it showed none inducible drug resistance to aquatic bacteria. Interestingly, it efficiently delayed ampicillin-induced drug resistance in Vibrio parahaemolyticus (sensitive to ampicillin) and sensitized ampicillin-resistant bacteria to ampicillin. The chequerboard assay indicated that topmouth culter LEAP-2 generated synergistic effects with ampicillin, indicating the combinational usage potential of topmouth culter LEAP-2 with antibiotics. As expected, topmouth culter LEAP-2 significantly alleviated ampicillin-resistant A. hydrophila infection in vivo , and enhanced the therapeutic efficacy of ampicillin against A. hydrophila in vivo . Our findings provide a fish innate immune system-derived peptide candidate for the substitute of antibiotics and highlight its potential for application in antibiotic-resistant bacterial infection in aquaculture industry." @default.
- W3107097029 created "2020-12-07" @default.
- W3107097029 creator A5005830319 @default.
- W3107097029 creator A5008540706 @default.
- W3107097029 creator A5011345322 @default.
- W3107097029 creator A5014894920 @default.
- W3107097029 creator A5044904833 @default.
- W3107097029 creator A5061119659 @default.
- W3107097029 creator A5061765389 @default.
- W3107097029 creator A5071537189 @default.
- W3107097029 creator A5086595514 @default.
- W3107097029 date "2020-11-23" @default.
- W3107097029 modified "2023-10-17" @default.
- W3107097029 title "Anti-infective Effects of a Fish-Derived Antimicrobial Peptide Against Drug-Resistant Bacteria and Its Synergistic Effects With Antibiotic" @default.
- W3107097029 cites W1605435161 @default.
- W3107097029 cites W1968200715 @default.
- W3107097029 cites W1982307201 @default.
- W3107097029 cites W2002019043 @default.
- W3107097029 cites W2022972180 @default.
- W3107097029 cites W2024399491 @default.
- W3107097029 cites W2047401331 @default.
- W3107097029 cites W2051006758 @default.
- W3107097029 cites W2054602599 @default.
- W3107097029 cites W2058110390 @default.
- W3107097029 cites W2059390726 @default.
- W3107097029 cites W2063282156 @default.
- W3107097029 cites W2070062955 @default.
- W3107097029 cites W2076549410 @default.
- W3107097029 cites W2077220947 @default.
- W3107097029 cites W2094711204 @default.
- W3107097029 cites W2103034068 @default.
- W3107097029 cites W2112502042 @default.
- W3107097029 cites W2121125797 @default.
- W3107097029 cites W2290750088 @default.
- W3107097029 cites W2337957188 @default.
- W3107097029 cites W2519490000 @default.
- W3107097029 cites W2538641106 @default.
- W3107097029 cites W2763778236 @default.
- W3107097029 cites W2770541357 @default.
- W3107097029 cites W2771966062 @default.
- W3107097029 cites W2801162566 @default.
- W3107097029 cites W2887241962 @default.
- W3107097029 cites W2909367641 @default.
- W3107097029 cites W2923128159 @default.
- W3107097029 cites W2936188727 @default.
- W3107097029 cites W2950390372 @default.
- W3107097029 cites W2994634742 @default.
- W3107097029 cites W3003742399 @default.
- W3107097029 doi "https://doi.org/10.3389/fmicb.2020.602412" @default.
- W3107097029 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7719739" @default.
- W3107097029 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33329494" @default.
- W3107097029 hasPublicationYear "2020" @default.
- W3107097029 type Work @default.
- W3107097029 sameAs 3107097029 @default.
- W3107097029 citedByCount "17" @default.
- W3107097029 countsByYear W31070970292021 @default.
- W3107097029 countsByYear W31070970292022 @default.
- W3107097029 countsByYear W31070970292023 @default.
- W3107097029 crossrefType "journal-article" @default.
- W3107097029 hasAuthorship W3107097029A5005830319 @default.
- W3107097029 hasAuthorship W3107097029A5008540706 @default.
- W3107097029 hasAuthorship W3107097029A5011345322 @default.
- W3107097029 hasAuthorship W3107097029A5014894920 @default.
- W3107097029 hasAuthorship W3107097029A5044904833 @default.
- W3107097029 hasAuthorship W3107097029A5061119659 @default.
- W3107097029 hasAuthorship W3107097029A5061765389 @default.
- W3107097029 hasAuthorship W3107097029A5071537189 @default.
- W3107097029 hasAuthorship W3107097029A5086595514 @default.
- W3107097029 hasBestOaLocation W31070970291 @default.
- W3107097029 hasConcept C176947019 @default.
- W3107097029 hasConcept C2776021129 @default.
- W3107097029 hasConcept C2778883795 @default.
- W3107097029 hasConcept C2779441561 @default.
- W3107097029 hasConcept C4937899 @default.
- W3107097029 hasConcept C501593827 @default.
- W3107097029 hasConcept C523546767 @default.
- W3107097029 hasConcept C540938839 @default.
- W3107097029 hasConcept C54355233 @default.
- W3107097029 hasConcept C86803240 @default.
- W3107097029 hasConcept C89423630 @default.
- W3107097029 hasConcept C94665300 @default.
- W3107097029 hasConceptScore W3107097029C176947019 @default.
- W3107097029 hasConceptScore W3107097029C2776021129 @default.
- W3107097029 hasConceptScore W3107097029C2778883795 @default.
- W3107097029 hasConceptScore W3107097029C2779441561 @default.
- W3107097029 hasConceptScore W3107097029C4937899 @default.
- W3107097029 hasConceptScore W3107097029C501593827 @default.
- W3107097029 hasConceptScore W3107097029C523546767 @default.
- W3107097029 hasConceptScore W3107097029C540938839 @default.
- W3107097029 hasConceptScore W3107097029C54355233 @default.
- W3107097029 hasConceptScore W3107097029C86803240 @default.
- W3107097029 hasConceptScore W3107097029C89423630 @default.
- W3107097029 hasConceptScore W3107097029C94665300 @default.
- W3107097029 hasFunder F4320321001 @default.
- W3107097029 hasLocation W31070970291 @default.
- W3107097029 hasLocation W31070970292 @default.
- W3107097029 hasLocation W31070970293 @default.
- W3107097029 hasOpenAccess W3107097029 @default.
- W3107097029 hasPrimaryLocation W31070970291 @default.
- W3107097029 hasRelatedWork W1598166495 @default.