FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3107258009> ?p ?o ?g. }

• W3107258009 endingPage "1336" @default.
• W3107258009 startingPage "1336.1" @default.
• W3107258009 abstract "Background: HE-1100 is a multicomponent medicinal product. Initial preclinical data potentially suggest a preventive effect on cartilage degradation. Objectives: This study aims to understand the mode of action of HE-1100 on OA chondrocytes in vitro . Methods: Primary chondrocytes were obtained from 10 knee osteoarthritis (OA) patients undergoing knee replacement surgery. The cultures were treated with 20% (v/v) HE-1100 or placebo. Samples were collected for subsequent RNA extraction using standard methods. The reads were generated with Illumina NextSeq5000 sequencer and aligned to the human reference genome (UCSC hg19) to generate the transcriptome. Differential expression analysis between HE-1100 and placebo was made in R using the DESeq2 package to identify the differentially expressed genes in the OA-associated regulatory pathways. The protein production of the selected genes was quantified by ELISA in 10 independent human OA chondrocytes cultures. Results: According to the DESeq2 analysis, HE-1100 significantly modified the expression of 13 genes in OA chondrocytes by at least 10% with an adjusted p-value < 0.05: EGR1 (+93%), FOS (+87%), NR4A1 (+43%), DUSP1 (+18%), ZFP36 (+18%), ZFP36L1 (+14%), NFKBIZ (+16%) and CYR61 (+14%) were upregulated and ATF7IP (-10%), TXNIP (-11%), C10orf10 (-12%), CLEC3A (-12%) and MMP13 (-18%) were downregulated after 24h HE-1100 treatment. HE-1100 significantly increased (2.3 fold +/-1.2 after 24h, p=0.0444 and 2.3-fold +/-1.0 after 72h, p=0.0239) the CYR61 protein production by human OA chondrocytes. After 72h, HE-1100 slightly but not significantly increased aggrecan production by 14 ± 19 % (p=0.1117) and significantly increased type II collagen pro-peptide production by 27 ± 20 % (p=0.0147). For both time points CYR61 production by OA chondrocytes was positively and significantly correlated with aggrecan (r=0.66, p=0.0004) and type II collagen pro-peptide (r=0.64, p=0.0008) production. In alginate beads culture, pro-MMP-13 was significantly decreased by HE-1100 treated cultures from day 7 to day 14 (from -16 to -25 %, p<0.05) and from day 17 to 21 (-22 %, p=0.0331) in comparison to controls. Conclusion: HE-1100 significantly modified the expression of DUSP1, C10orf10, ZFP36/L1 and CLEC3A, which are pathway mediators involved in MMP-13 expression and activation. Further, long-term (28 days) treatment with HE-1100 significantly reduced the production of pro-MMP-13, the inactive precursor of the metalloproteinase MMP-13 involved in type II collagen degradation. HE-1100 also promoted extracellular matrix formation probably through CYR61 production, a growth factor well correlated with type II collagen and aggrecan production. References: / Acknowledgments: We would like to thank the staff of the GIGA ULiège Genomic Next Generation Sequencing platform for performing the RNA sequencing and Benoit Charloteaux for his help in RNAseq data analysis. Disclosure of Interests: christelle sanchez: None declared, Kathrin Hemmer Employee of: Heel, Natascha Kroemmelbein Employee of: Heel, Bernd Seilheimer Employee of: Heel, Jean-Emile Dubuc: None declared, Christophe Antoine Employee of: Artialis, Yves Henrotin Grant/research support from: HEEL, TILMAN" @default.
• W3107258009 created "2020-12-07" @default.
• W3107258009 creator A5013195734 @default.
• W3107258009 creator A5027325896 @default.
• W3107258009 creator A5040319350 @default.
• W3107258009 creator A5050755647 @default.
• W3107258009 creator A5058161894 @default.
• W3107258009 creator A5071267851 @default.
• W3107258009 creator A5078070582 @default.
• W3107258009 date "2020-06-01" @default.
• W3107258009 modified "2023-09-23" @default.
• W3107258009 title "AB0072 A MULTICOMPONENT MEDICATION PROMOTES CHONDROGENESIS AND REDUCES MMP-13 IN PRIMARY ARTICULAR CHONDROCYTES FROM KNEE OSTEOARTHRITIS PATIENTS IN VITRO" @default.
• W3107258009 doi "https://doi.org/10.1136/annrheumdis-2020-eular.2106" @default.
• W3107258009 hasPublicationYear "2020" @default.
• W3107258009 type Work @default.
• W3107258009 sameAs 3107258009 @default.
• W3107258009 citedByCount "0" @default.
• W3107258009 crossrefType "journal-article" @default.
• W3107258009 hasAuthorship W3107258009A5013195734 @default.
• W3107258009 hasAuthorship W3107258009A5027325896 @default.
• W3107258009 hasAuthorship W3107258009A5040319350 @default.
• W3107258009 hasAuthorship W3107258009A5050755647 @default.
• W3107258009 hasAuthorship W3107258009A5058161894 @default.
• W3107258009 hasAuthorship W3107258009A5071267851 @default.
• W3107258009 hasAuthorship W3107258009A5078070582 @default.
• W3107258009 hasBestOaLocation W31072580091 @default.
• W3107258009 hasConcept C104317684 @default.
• W3107258009 hasConcept C105702510 @default.
• W3107258009 hasConcept C126322002 @default.
• W3107258009 hasConcept C127561419 @default.
• W3107258009 hasConcept C142724271 @default.
• W3107258009 hasConcept C150194340 @default.
• W3107258009 hasConcept C153911025 @default.
• W3107258009 hasConcept C162317418 @default.
• W3107258009 hasConcept C197534660 @default.
• W3107258009 hasConcept C202751555 @default.
• W3107258009 hasConcept C204787440 @default.
• W3107258009 hasConcept C2776164576 @default.
• W3107258009 hasConcept C2780550940 @default.
• W3107258009 hasConcept C3020332539 @default.
• W3107258009 hasConcept C35496256 @default.
• W3107258009 hasConcept C55493867 @default.
• W3107258009 hasConcept C71924100 @default.
• W3107258009 hasConcept C86803240 @default.
• W3107258009 hasConceptScore W3107258009C104317684 @default.
• W3107258009 hasConceptScore W3107258009C105702510 @default.
• W3107258009 hasConceptScore W3107258009C126322002 @default.
• W3107258009 hasConceptScore W3107258009C127561419 @default.
• W3107258009 hasConceptScore W3107258009C142724271 @default.
• W3107258009 hasConceptScore W3107258009C150194340 @default.
• W3107258009 hasConceptScore W3107258009C153911025 @default.
• W3107258009 hasConceptScore W3107258009C162317418 @default.
• W3107258009 hasConceptScore W3107258009C197534660 @default.
• W3107258009 hasConceptScore W3107258009C202751555 @default.
• W3107258009 hasConceptScore W3107258009C204787440 @default.
• W3107258009 hasConceptScore W3107258009C2776164576 @default.
• W3107258009 hasConceptScore W3107258009C2780550940 @default.
• W3107258009 hasConceptScore W3107258009C3020332539 @default.
• W3107258009 hasConceptScore W3107258009C35496256 @default.
• W3107258009 hasConceptScore W3107258009C55493867 @default.
• W3107258009 hasConceptScore W3107258009C71924100 @default.
• W3107258009 hasConceptScore W3107258009C86803240 @default.
• W3107258009 hasIssue "Suppl 1" @default.
• W3107258009 hasLocation W31072580091 @default.
• W3107258009 hasOpenAccess W3107258009 @default.
• W3107258009 hasPrimaryLocation W31072580091 @default.
• W3107258009 hasRelatedWork W2026734181 @default.
• W3107258009 hasRelatedWork W2030780288 @default.
• W3107258009 hasRelatedWork W2039221240 @default.
• W3107258009 hasRelatedWork W2044607996 @default.
• W3107258009 hasRelatedWork W2139452459 @default.
• W3107258009 hasRelatedWork W2173165013 @default.
• W3107258009 hasRelatedWork W2404897701 @default.
• W3107258009 hasRelatedWork W2486727342 @default.
• W3107258009 hasRelatedWork W2917702969 @default.
• W3107258009 hasRelatedWork W4206330498 @default.
• W3107258009 hasVolume "79" @default.
• W3107258009 isParatext "false" @default.
• W3107258009 isRetracted "false" @default.
• W3107258009 magId "3107258009" @default.
• W3107258009 workType "article" @default.