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• W3107259064 endingPage "463" @default.
• W3107259064 startingPage "448" @default.
• W3107259064 abstract "Heme-nitric oxide/oxygen binding (H-NOX) domains bind gaseous ligands for signal transduction in organisms spanning prokaryotic and eukaryotic kingdoms. In the bioluminescent marine bacterium Shewanella woodyi (Sw), H-NOX proteins regulate quorum sensing and biofilm formation. In higher animals, soluble guanylyl cyclase (sGC) binds nitric oxide with an H-NOX domain to induce cyclase activity and regulate vascular tone, wound healing and memory formation. sGC also binds stimulator compounds targeting cardiovascular disease. The molecular details of stimulator binding to sGC remain obscure but involve a binding pocket near an interface between H-NOX and coiled-coil domains. Here, we report the full NMR structure for CO-ligated Sw H-NOX in the presence and absence of stimulator compound IWP-051, and its backbone dynamics. Nonplanar heme geometry was retained using a semi-empirical quantum potential energy approach. Although IWP-051 binding is weak, a single binding conformation was found at the interface of the two H-NOX subdomains, near but not overlapping with sites identified in sGC. Binding leads to rotation of the subdomains and closure of the binding pocket. Backbone dynamics are similar across both domains except for two helix-connecting loops, which display increased dynamics that are further enhanced by compound binding. Structure-based sequence analyses indicate high sequence diversity in the binding pocket, but the pocket itself appears conserved among H-NOX proteins. The largest dynamical loop lies at the interface between Sw H-NOX and its binding partner as well as in the interface with the coiled coil in sGC, suggesting a critical role for the loop in signal transduction." @default.
• W3107259064 created "2020-12-07" @default.
• W3107259064 creator A5006564372 @default.
• W3107259064 creator A5006822373 @default.
• W3107259064 creator A5011988253 @default.
• W3107259064 creator A5043970745 @default.
• W3107259064 creator A5052591725 @default.
• W3107259064 date "2020-12-10" @default.
• W3107259064 modified "2023-10-14" @default.
• W3107259064 title "Solution structures of the <i>Shewanella woodyi</i> <scp>H‐NOX</scp> protein in the presence and absence of soluble guanylyl cyclase stimulator <scp>IWP</scp> ‐051" @default.
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• W3107259064 doi "https://doi.org/10.1002/pro.4005" @default.
• W3107259064 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7784750" @default.
• W3107259064 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33236796" @default.
• W3107259064 hasPublicationYear "2020" @default.
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