FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3107748881> ?p ?o ?g. }

• W3107748881 endingPage "3363" @default.
• W3107748881 startingPage "3353" @default.
• W3107748881 abstract "Transcriptional perturbation using inactivated CRISPR-nucleases (dCas) is a common method in eukaryotic organisms. While rare examples of dCas9-based tools for prokaryotes have been described, multiplexing approaches are limited due to the used effector nuclease. For the first time, a dCas12a derived tool for the targeted activation and repression of genes was developed. Therefore, a previously described SoxS activator domain was linked to dCas12a to enable the programmable activation of gene expression. A proof of principle of transcriptional regulation was demonstrated on the basis of fluorescence reporter assays using the alternative host organism Paenibacillus polymyxa as well as Escherichia coli. Single target and multiplex CRISPR interference targeting the exopolysaccharide biosynthesis of P. polymyxa was shown to emulate polymer compositions of gene knockouts. The simultaneous expression of 11 gRNAs targeting multiple lactate dehydrogenases and a butanediol dehydrogenase resulted in decreased lactate formation, as well as an increased butanediol production in microaerobic fermentation processes. Even though Cas12a is more restricted in terms of its genomic target sequences compared to Cas9, its ability to efficiently process its own guide RNAs in vivo makes it a promising tool to orchestrate sophisticated genetic reprogramming of bacterial cells or to screen for engineering targets in the genome. The developed tool will accelerate metabolic engineering efforts in the alternative host organism P. polymyxa and might be also applied for other bacterial cell factories." @default.
• W3107748881 created "2020-12-07" @default.
• W3107748881 creator A5019099649 @default.
• W3107748881 creator A5056167556 @default.
• W3107748881 creator A5074195161 @default.
• W3107748881 creator A5083816004 @default.
• W3107748881 date "2020-11-25" @default.
• W3107748881 modified "2023-10-18" @default.
• W3107748881 title "Novel Prokaryotic CRISPR-Cas12a-Based Tool for Programmable Transcriptional Activation and Repression" @default.
• W3107748881 cites W1264699681 @default.
• W3107748881 cites W1731173485 @default.
• W3107748881 cites W1971439989 @default.
• W3107748881 cites W1975837950 @default.
• W3107748881 cites W1989568784 @default.
• W3107748881 cites W1992783297 @default.
• W3107748881 cites W1999130463 @default.
• W3107748881 cites W1999863614 @default.
• W3107748881 cites W2000046950 @default.
• W3107748881 cites W2018532552 @default.
• W3107748881 cites W2028543816 @default.
• W3107748881 cites W2035420256 @default.
• W3107748881 cites W2043055543 @default.
• W3107748881 cites W2053609834 @default.
• W3107748881 cites W2061650108 @default.
• W3107748881 cites W2080865798 @default.
• W3107748881 cites W2089694025 @default.
• W3107748881 cites W2090660721 @default.
• W3107748881 cites W2101749257 @default.
• W3107748881 cites W2107277218 @default.
• W3107748881 cites W2111014820 @default.
• W3107748881 cites W2111612750 @default.
• W3107748881 cites W2120674407 @default.
• W3107748881 cites W2120932131 @default.
• W3107748881 cites W2149202606 @default.
• W3107748881 cites W2152505144 @default.
• W3107748881 cites W2169373177 @default.
• W3107748881 cites W2204875887 @default.
• W3107748881 cites W2293809279 @default.
• W3107748881 cites W2337626698 @default.
• W3107748881 cites W2341405505 @default.
• W3107748881 cites W2539030633 @default.
• W3107748881 cites W2560230043 @default.
• W3107748881 cites W2565333691 @default.
• W3107748881 cites W2569653953 @default.
• W3107748881 cites W2623910725 @default.
• W3107748881 cites W2768075868 @default.
• W3107748881 cites W2768861378 @default.
• W3107748881 cites W2780422845 @default.
• W3107748881 cites W2793309516 @default.
• W3107748881 cites W2794688631 @default.
• W3107748881 cites W2808828797 @default.
• W3107748881 cites W2878721160 @default.
• W3107748881 cites W2901495854 @default.
• W3107748881 cites W2915013877 @default.
• W3107748881 cites W2920923539 @default.
• W3107748881 cites W2963739921 @default.
• W3107748881 cites W2966542918 @default.
• W3107748881 cites W2967705142 @default.
• W3107748881 cites W2969515168 @default.
• W3107748881 cites W2971842044 @default.
• W3107748881 cites W2989794628 @default.
• W3107748881 cites W3041482157 @default.
• W3107748881 cites W3047123218 @default.
• W3107748881 cites W4379389746 @default.
• W3107748881 cites W826124742 @default.
• W3107748881 doi "https://doi.org/10.1021/acssynbio.0c00424" @default.
• W3107748881 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33238093" @default.
• W3107748881 hasPublicationYear "2020" @default.
• W3107748881 type Work @default.
• W3107748881 sameAs 3107748881 @default.
• W3107748881 citedByCount "16" @default.
• W3107748881 countsByYear W31077488812021 @default.
• W3107748881 countsByYear W31077488812022 @default.
• W3107748881 countsByYear W31077488812023 @default.
• W3107748881 crossrefType "journal-article" @default.
• W3107748881 hasAuthorship W3107748881A5019099649 @default.
• W3107748881 hasAuthorship W3107748881A5056167556 @default.
• W3107748881 hasAuthorship W3107748881A5074195161 @default.
• W3107748881 hasAuthorship W3107748881A5083816004 @default.
• W3107748881 hasBestOaLocation W31077488812 @default.
• W3107748881 hasConcept C104317684 @default.
• W3107748881 hasConcept C132455925 @default.
• W3107748881 hasConcept C144501496 @default.
• W3107748881 hasConcept C145290725 @default.
• W3107748881 hasConcept C154055011 @default.
• W3107748881 hasConcept C191908910 @default.
• W3107748881 hasConcept C2777271071 @default.
• W3107748881 hasConcept C2779134421 @default.
• W3107748881 hasConcept C2781188995 @default.
• W3107748881 hasConcept C31909778 @default.
• W3107748881 hasConcept C523546767 @default.
• W3107748881 hasConcept C54355233 @default.
• W3107748881 hasConcept C6350086 @default.
• W3107748881 hasConcept C70721500 @default.
• W3107748881 hasConcept C84356384 @default.
• W3107748881 hasConcept C86803240 @default.
• W3107748881 hasConcept C97702854 @default.
• W3107748881 hasConcept C98108389 @default.

• next