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- W3107781897 abstract "Fusion of vesicles with the plasma membrane and liberation of their contents is a multistep process involving several proteins. Correctly assigning the role of specific proteins and reactions in this cascade requires a measurement method with high temporal resolution. Patch-clamp recordings of cell membrane capacitance in combination with calcium measurements, calcium uncaging, and carbon-fiber amperometry allow for the accurate determination of vesicle pool sizes, their fusion kinetics, and their secreted oxidizable content. Here, we will describe this method in a model system for neurosecretion, the adrenal chromaffin cells, which secrete adrenaline." @default.
- W3107781897 created "2020-12-07" @default.
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- W3107781897 date "2020-11-22" @default.
- W3107781897 modified "2023-09-23" @default.
- W3107781897 title "Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging in Mouse Adrenal Chromaffin Cells" @default.
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- W3107781897 doi "https://doi.org/10.1007/978-1-0716-1044-2_16" @default.
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