FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3107787921> ?p ?o ?g. }

• W3107787921 endingPage "e2290" @default.
• W3107787921 startingPage "e2290" @default.
• W3107787921 abstract "COVID-19 is a public health emergency of international concern whose detection in recovered asymptomatic patients is dependent on accurate diagnosis as it enables the estimation of the susceptibility of the population to the infection. This demand has resulted in the development of several commercial assays employing recombinant proteins, but the results of these assays are not reliable as they do not involve comparison with natural viral antigens. We independently used the SARS-CoV-2 whole viral antigen (WVA) and recombinant nucleocapsid protein (rNP) to develop in-house ELISAs for IgG detection; the results of these ELISAs were then compared to obtain reliable results.WVA and rNP ELISAs were performed on COVID-19 negative sera from patients before the pandemic in Brazil, and on RT-qPCR-positive or SARS-CoV-2-IgG against rNP and IgG against WVA-positive samples from recently infected patients in Sao Paulo, Brazil.Both ELISAs detected a large fraction of infected patients but exhibited certain drawbacks. Higher signals and lower numbers of false-negatives were observed in rNP ELISA; however, a higher fraction of false-positives was observed in control groups. A high number of false-negatives was observed with WVA ELISA. Correlating the results of rNP and WVA ELISAs resulted in improved performance for COVID-19 diagnosis.The choice of antigen is an important aspect in optimizing the laboratory diagnosis of COVID-19. The use of rNP ELISA for the detection of anti-SARS-CoV-2 IgG antibodies seems promising, but comparison of the results with those of WVA ELISA is crucial for accurate test development prior to commercialization. IgG serology using several assays, and with the spectral patterns of SARS-CoV-2, resulted in confusing information that must be clarified before the establishment of diagnostic serology criteria." @default.
• W3107787921 created "2020-12-07" @default.
• W3107787921 creator A5024090944 @default.
• W3107787921 creator A5025450903 @default.
• W3107787921 creator A5032593064 @default.
• W3107787921 creator A5039556331 @default.
• W3107787921 creator A5041531713 @default.
• W3107787921 creator A5049800755 @default.
• W3107787921 creator A5058085588 @default.
• W3107787921 creator A5060896241 @default.
• W3107787921 creator A5065959170 @default.
• W3107787921 creator A5080383372 @default.
• W3107787921 creator A5084049447 @default.
• W3107787921 creator A5089589980 @default.
• W3107787921 date "2020-01-01" @default.
• W3107787921 modified "2023-10-14" @default.
• W3107787921 title "Natural versus Recombinant Viral Antigens in SARS-CoV-2 Serology: Challenges in Optimizing Laboratory Diagnosis of COVID-19" @default.
• W3107787921 cites W2029489594 @default.
• W3107787921 cites W2046502440 @default.
• W3107787921 cites W2069717299 @default.
• W3107787921 cites W2084580119 @default.
• W3107787921 cites W2116586125 @default.
• W3107787921 cites W2560409032 @default.
• W3107787921 cites W2911273095 @default.
• W3107787921 cites W3004280078 @default.
• W3107787921 cites W3006703916 @default.
• W3107787921 cites W3007104924 @default.
• W3107787921 cites W3008027968 @default.
• W3107787921 cites W3010233963 @default.
• W3107787921 cites W3010466793 @default.
• W3107787921 cites W3010604545 @default.
• W3107787921 cites W3011420630 @default.
• W3107787921 cites W3012569595 @default.
• W3107787921 cites W3016611530 @default.
• W3107787921 cites W3016924729 @default.
• W3107787921 cites W3017325113 @default.
• W3107787921 cites W3018274661 @default.
• W3107787921 cites W3019765297 @default.
• W3107787921 cites W3021274772 @default.
• W3107787921 cites W3029055936 @default.
• W3107787921 cites W3032292222 @default.
• W3107787921 cites W3035201735 @default.
• W3107787921 cites W3038707046 @default.
• W3107787921 cites W3087128437 @default.
• W3107787921 cites W3094307568 @default.
• W3107787921 doi "https://doi.org/10.6061/clinics/2020/e2290" @default.
• W3107787921 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7690966" @default.
• W3107787921 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33331401" @default.
• W3107787921 hasPublicationYear "2020" @default.
• W3107787921 type Work @default.
• W3107787921 sameAs 3107787921 @default.
• W3107787921 citedByCount "3" @default.
• W3107787921 countsByYear W31077879212021 @default.
• W3107787921 countsByYear W31077879212022 @default.
• W3107787921 crossrefType "journal-article" @default.
• W3107787921 hasAuthorship W3107787921A5024090944 @default.
• W3107787921 hasAuthorship W3107787921A5025450903 @default.
• W3107787921 hasAuthorship W3107787921A5032593064 @default.
• W3107787921 hasAuthorship W3107787921A5039556331 @default.
• W3107787921 hasAuthorship W3107787921A5041531713 @default.
• W3107787921 hasAuthorship W3107787921A5049800755 @default.
• W3107787921 hasAuthorship W3107787921A5058085588 @default.
• W3107787921 hasAuthorship W3107787921A5060896241 @default.
• W3107787921 hasAuthorship W3107787921A5065959170 @default.
• W3107787921 hasAuthorship W3107787921A5080383372 @default.
• W3107787921 hasAuthorship W3107787921A5084049447 @default.
• W3107787921 hasAuthorship W3107787921A5089589980 @default.
• W3107787921 hasBestOaLocation W31077879211 @default.
• W3107787921 hasConcept C104317684 @default.
• W3107787921 hasConcept C126322002 @default.
• W3107787921 hasConcept C147483822 @default.
• W3107787921 hasConcept C159047783 @default.
• W3107787921 hasConcept C159654299 @default.
• W3107787921 hasConcept C203014093 @default.
• W3107787921 hasConcept C2777910003 @default.
• W3107787921 hasConcept C2779134260 @default.
• W3107787921 hasConcept C2908647359 @default.
• W3107787921 hasConcept C3007834351 @default.
• W3107787921 hasConcept C3008058167 @default.
• W3107787921 hasConcept C40767141 @default.
• W3107787921 hasConcept C45189115 @default.
• W3107787921 hasConcept C524204448 @default.
• W3107787921 hasConcept C55493867 @default.
• W3107787921 hasConcept C71924100 @default.
• W3107787921 hasConcept C86803240 @default.
• W3107787921 hasConcept C99454951 @default.
• W3107787921 hasConceptScore W3107787921C104317684 @default.
• W3107787921 hasConceptScore W3107787921C126322002 @default.
• W3107787921 hasConceptScore W3107787921C147483822 @default.
• W3107787921 hasConceptScore W3107787921C159047783 @default.
• W3107787921 hasConceptScore W3107787921C159654299 @default.
• W3107787921 hasConceptScore W3107787921C203014093 @default.
• W3107787921 hasConceptScore W3107787921C2777910003 @default.
• W3107787921 hasConceptScore W3107787921C2779134260 @default.
• W3107787921 hasConceptScore W3107787921C2908647359 @default.
• W3107787921 hasConceptScore W3107787921C3007834351 @default.
• W3107787921 hasConceptScore W3107787921C3008058167 @default.
• W3107787921 hasConceptScore W3107787921C40767141 @default.

• next