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- W3108020086 abstract "Cow's milk allergy is one of the most prevalent food allergies in both children and adults. As dairy products are common dietary ingredients and the prevalence of chronic conditions is on the rise, milk allergy is a growing public health concern. To elucidate underlying mechanisms and develop therapeutic strategies, reliable animal models are essential research tools. Sensitization to a milk protein is the principal procedure for establishing animal models of cow's milk allergy. However, the methods of sensitization vary from laboratory to laboratory, using different milk proteins with different amounts, routes, and durations of allergen exposure during sensitization of varying sex and strains of mice, likely resulting in diverse immunological and physical responses. Furthermore, the sources and potential impurities of milk protein may also produce variable responses. Thus, standardization of sensitization protocol is important, particularly when results are compared across studies. Here, we describe a method to generate a mouse model of cow's milk allergy using purified β-lactoglobulin as the milk allergen with cholera toxin as an adjuvant in a 5-week oral sensitization protocol." @default.
- W3108020086 created "2020-12-07" @default.
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- W3108020086 date "2020-11-24" @default.
- W3108020086 modified "2023-10-15" @default.
- W3108020086 title "Induction of Hypersensitivity with Purified Beta-Lactoglobulin as a Mouse Model of Cow’s Milk Allergy" @default.
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- W3108020086 doi "https://doi.org/10.1007/978-1-0716-1001-5_5" @default.
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