Matches in SemOpenAlex for { <https://semopenalex.org/work/W3108351386> ?p ?o ?g. }
- W3108351386 abstract "Abstract Investigating the complex interactions between stem cells and their native environment requires an efficient means to image them in situ . Caenorhabditis elegans germline stem cells (GSCs) are distinctly accessible for intravital imaging; however, long-term image acquisition and analysis of dividing GSCs can be technically challenging. Here we present a systematic investigation into the technical factors impacting GSC physiology during live imaging and provide an optimized method for monitoring GSC mitosis under minimally disruptive conditions. We describe CentTracker, an automated and generalizable image analysis tool that uses machine learning to pair mitotic centrosomes and which can extract a variety of mitotic parameters rapidly from large-scale datasets. We employ CentTracker to assess a range of mitotic features in GSCs and show that subpopulations with distinct mitotic profiles are unlikely to exist within the stem cell pool. We further find evidence for spatial clustering of GSC mitoses within the germline tissue and for biases in mitotic spindle orientation relative to the germline’s distal-proximal axis, and thus the niche. The technical and analytical tools provided herein pave the way for large-scale screening studies of multiple mitotic processes in GSCs dividing in situ , in an intact tissue, in a living animal, under seemingly physiological conditions." @default.
- W3108351386 created "2020-12-07" @default.
- W3108351386 creator A5011445343 @default.
- W3108351386 creator A5015587261 @default.
- W3108351386 creator A5067965301 @default.
- W3108351386 creator A5087229969 @default.
- W3108351386 creator A5087241634 @default.
- W3108351386 creator A5089304873 @default.
- W3108351386 date "2020-11-22" @default.
- W3108351386 modified "2023-10-16" @default.
- W3108351386 title "CentTracker: a trainable, machine learning-based tool for large-scale analyses of C. elegans germline stem cell mitosis" @default.
- W3108351386 cites W1565994303 @default.
- W3108351386 cites W1968426398 @default.
- W3108351386 cites W1975022818 @default.
- W3108351386 cites W1980735976 @default.
- W3108351386 cites W1981433220 @default.
- W3108351386 cites W1984944970 @default.
- W3108351386 cites W1986006829 @default.
- W3108351386 cites W2000197813 @default.
- W3108351386 cites W2007496445 @default.
- W3108351386 cites W2008674896 @default.
- W3108351386 cites W2009691273 @default.
- W3108351386 cites W2015159529 @default.
- W3108351386 cites W2016540253 @default.
- W3108351386 cites W2018264362 @default.
- W3108351386 cites W2018868264 @default.
- W3108351386 cites W2034556762 @default.
- W3108351386 cites W2038029561 @default.
- W3108351386 cites W2038492783 @default.
- W3108351386 cites W2040453897 @default.
- W3108351386 cites W2053704624 @default.
- W3108351386 cites W2056398241 @default.
- W3108351386 cites W2085454685 @default.
- W3108351386 cites W2086265207 @default.
- W3108351386 cites W2087849201 @default.
- W3108351386 cites W2091441160 @default.
- W3108351386 cites W2091992164 @default.
- W3108351386 cites W2098085839 @default.
- W3108351386 cites W2101832805 @default.
- W3108351386 cites W2107518572 @default.
- W3108351386 cites W2109796319 @default.
- W3108351386 cites W2112855374 @default.
- W3108351386 cites W2113160211 @default.
- W3108351386 cites W2114839546 @default.
- W3108351386 cites W2116352427 @default.
- W3108351386 cites W2132162535 @default.
- W3108351386 cites W2135625048 @default.
- W3108351386 cites W2138700610 @default.
- W3108351386 cites W2140706307 @default.
- W3108351386 cites W2146292423 @default.
- W3108351386 cites W2157149380 @default.
- W3108351386 cites W2159565935 @default.
- W3108351386 cites W2162448144 @default.
- W3108351386 cites W2163871471 @default.
- W3108351386 cites W2166622823 @default.
- W3108351386 cites W2167279371 @default.
- W3108351386 cites W2168830217 @default.
- W3108351386 cites W2236822702 @default.
- W3108351386 cites W2399785989 @default.
- W3108351386 cites W2406250479 @default.
- W3108351386 cites W2465388906 @default.
- W3108351386 cites W2490379935 @default.
- W3108351386 cites W2509653185 @default.
- W3108351386 cites W2529052661 @default.
- W3108351386 cites W2567058169 @default.
- W3108351386 cites W2587071877 @default.
- W3108351386 cites W2588549795 @default.
- W3108351386 cites W2791362637 @default.
- W3108351386 cites W2795472155 @default.
- W3108351386 cites W2807818417 @default.
- W3108351386 cites W2917725672 @default.
- W3108351386 cites W2992563067 @default.
- W3108351386 cites W3044437722 @default.
- W3108351386 cites W3099878876 @default.
- W3108351386 cites W4242673802 @default.
- W3108351386 cites W4300009529 @default.
- W3108351386 doi "https://doi.org/10.1101/2020.11.22.393272" @default.
- W3108351386 hasPublicationYear "2020" @default.
- W3108351386 type Work @default.
- W3108351386 sameAs 3108351386 @default.
- W3108351386 citedByCount "0" @default.
- W3108351386 crossrefType "posted-content" @default.
- W3108351386 hasAuthorship W3108351386A5011445343 @default.
- W3108351386 hasAuthorship W3108351386A5015587261 @default.
- W3108351386 hasAuthorship W3108351386A5067965301 @default.
- W3108351386 hasAuthorship W3108351386A5087229969 @default.
- W3108351386 hasAuthorship W3108351386A5087241634 @default.
- W3108351386 hasAuthorship W3108351386A5089304873 @default.
- W3108351386 hasBestOaLocation W31083513861 @default.
- W3108351386 hasConcept C104317684 @default.
- W3108351386 hasConcept C10447061 @default.
- W3108351386 hasConcept C109825262 @default.
- W3108351386 hasConcept C121332964 @default.
- W3108351386 hasConcept C1491633281 @default.
- W3108351386 hasConcept C2778755073 @default.
- W3108351386 hasConcept C2778944004 @default.
- W3108351386 hasConcept C28328180 @default.
- W3108351386 hasConcept C29537977 @default.
- W3108351386 hasConcept C54355233 @default.
- W3108351386 hasConcept C62520636 @default.