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• W3108448221 abstract "Abstract Candida tropicalis is a human pathogen that primarily infects the immunocompromised. Whereas the genome of one isolate, C. tropicalis MYA-3404, was originally sequenced in 2009, there have been no large-scale, multi-isolate studies of the genetic and phenotypic diversity of this species. Here, we used whole genome sequencing and phenotyping to characterize 77 isolates C. tropicalis isolates from clinical and environmental sources from a variety of locations. We show that most C. tropicalis isolates are diploids with approximately 2 - 6 heterozygous variants per kilobase. The genomes are relatively stable, with few aneuploidies. However, we identified one highly homozygous isolate and six isolates of C. tropicalis with much higher heterozygosity levels ranging from 36 - 49 heterozygous variants per kilobase. Our analyses show that the heterozygous isolates represent two different hybrid lineages, where the hybrids share one parent (A) with most other C. tropicalis isolates, but the second parent (B or C) differs by at least 4% at the genome level. Four of the sequenced isolates descend from an AB hybridization, and two from an AC hybridization. The hybrids are MTL a / α heterozygotes. Hybridization, or mating, between different parents is therefore common in the evolutionary history of C. tropicalis . The new hybrids were predominantly found in environmental niches, including from soil. Hybridization is therefore unlikely to be associated with virulence. In addition, we used genotype-phenotype correlation and CRISPR-Cas9 editing to identify a genome variant that results in the inability of one isolate to utilize certain branched-chain amino acids as a sole nitrogen source. Author summary Candida tropicalis is an important fungal pathogen, which is particularly common in the Asia-Pacific and Latin America. There is currently very little known about the diversity of genotype and phenotype of C. tropicalis isolates. By carrying out a phylogenomic analysis of 77 isolates, we find that C. tropicalis genomes range from very homozygous to highly heterozygous. We show that the heterozygous isolates are hybrids, most likely formed by mating between different parents. Unlike other Candida species, the hybrids are more common in environmental than in clinical niches, suggesting that for this species, hybridization is not associated with virulence. We also explore the range of phenotypes, and we identify a genomic variant that is required for growth on valine and isoleucine as sole nitrogen sources." @default.
• W3108448221 created "2020-12-07" @default.
• W3108448221 creator A5000225841 @default.
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• W3108448221 creator A5043867868 @default.
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• W3108448221 creator A5079361647 @default.
• W3108448221 creator A5089155974 @default.
• W3108448221 date "2020-11-26" @default.
• W3108448221 modified "2023-09-27" @default.
• W3108448221 title "Population genomics of the pathogenic yeast Candida tropicalis identifies hybrid isolates in environmental samples" @default.
• W3108448221 cites W1590529516 @default.
• W3108448221 cites W1641544182 @default.
• W3108448221 cites W1828054788 @default.
• W3108448221 cites W1900534157 @default.
• W3108448221 cites W1915062133 @default.
• W3108448221 cites W1923694921 @default.
• W3108448221 cites W1974342169 @default.
• W3108448221 cites W1981396590 @default.
• W3108448221 cites W1982727409 @default.
• W3108448221 cites W1996687891 @default.
• W3108448221 cites W2002005828 @default.
• W3108448221 cites W2002525133 @default.
• W3108448221 cites W2007746831 @default.
• W3108448221 cites W2011310198 @default.
• W3108448221 cites W2021488480 @default.
• W3108448221 cites W2021960948 @default.
• W3108448221 cites W2036743290 @default.
• W3108448221 cites W2049767049 @default.
• W3108448221 cites W2059302109 @default.
• W3108448221 cites W2067651829 @default.
• W3108448221 cites W2074335138 @default.
• W3108448221 cites W2077088073 @default.
• W3108448221 cites W2077724722 @default.
• W3108448221 cites W2081933515 @default.
• W3108448221 cites W2083481962 @default.
• W3108448221 cites W2085726024 @default.
• W3108448221 cites W2087348828 @default.
• W3108448221 cites W2087561016 @default.
• W3108448221 cites W2097054939 @default.
• W3108448221 cites W2102278945 @default.
• W3108448221 cites W2102619694 @default.
• W3108448221 cites W2106042067 @default.
• W3108448221 cites W2106578986 @default.
• W3108448221 cites W2107546215 @default.
• W3108448221 cites W2107772251 @default.
• W3108448221 cites W2108234281 @default.
• W3108448221 cites W2110903706 @default.
• W3108448221 cites W2114619345 @default.
• W3108448221 cites W2116486447 @default.
• W3108448221 cites W2117732943 @default.
• W3108448221 cites W2119180969 @default.
• W3108448221 cites W2119451273 @default.
• W3108448221 cites W2120902911 @default.
• W3108448221 cites W2122716163 @default.
• W3108448221 cites W2126392954 @default.
• W3108448221 cites W2130517250 @default.
• W3108448221 cites W2131213513 @default.
• W3108448221 cites W2136365742 @default.
• W3108448221 cites W2140646298 @default.
• W3108448221 cites W2140968247 @default.
• W3108448221 cites W2141052558 @default.
• W3108448221 cites W2147937067 @default.
• W3108448221 cites W2160734881 @default.
• W3108448221 cites W2163938253 @default.
• W3108448221 cites W2165415564 @default.
• W3108448221 cites W2165891191 @default.
• W3108448221 cites W2167188257 @default.
• W3108448221 cites W2167510791 @default.
• W3108448221 cites W2169540909 @default.
• W3108448221 cites W2170087779 @default.
• W3108448221 cites W2170392103 @default.
• W3108448221 cites W2171775455 @default.
• W3108448221 cites W2295980213 @default.
• W3108448221 cites W2338633945 @default.
• W3108448221 cites W2349021332 @default.
• W3108448221 cites W2522491625 @default.
• W3108448221 cites W2527740469 @default.
• W3108448221 cites W2547473733 @default.
• W3108448221 cites W2560383108 @default.
• W3108448221 cites W2602369823 @default.
• W3108448221 cites W2666643556 @default.
• W3108448221 cites W2731392871 @default.
• W3108448221 cites W2772906561 @default.
• W3108448221 cites W2790754851 @default.
• W3108448221 cites W2798064943 @default.
• W3108448221 cites W2802109134 @default.
• W3108448221 cites W2802789837 @default.
• W3108448221 cites W2805097482 @default.
• W3108448221 cites W2886523240 @default.
• W3108448221 cites W2897778894 @default.
• W3108448221 cites W2898110413 @default.
• W3108448221 cites W2908916169 @default.
• W3108448221 cites W2918666832 @default.
• W3108448221 cites W2922388167 @default.
• W3108448221 cites W2922672649 @default.
• W3108448221 cites W2941715396 @default.

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