Matches in SemOpenAlex for { <https://semopenalex.org/work/W3108968498> ?p ?o ?g. }
- W3108968498 abstract "Abstract PriA is a member of the SuperFamily 2 helicase family. Its role in vivo is to reload the primosome onto stalled replication forks resulting in the restart of the previously stalled DNA replication process. SSB is known to play key roles in mediating activities at replication forks and it is known to bind to PriA. To gain mechanistic insight into the PriA-SSB interaction, a coupled spectrophotometric assay was utilized to characterize the ATPase activity of PriA in vitro in the presence of fork substrates. The results demonstrate that SSB enhances the ability of PriA to discriminate between fork substrates 140-fold. This is due to a significant increase in the catalytic efficiency of the helicase induced by DNA-bound SSB. This interaction is species-specific as bacteriophage gene 32 protein cannot substitute for the E.coli protein. SSB, while enhancing the activity of PriA on its preferred fork, both decreases the affinity of the helicase for other forks and decreases catalytic efficiency. Central to the stimulation afforded by SSB is the unique ability of PriA to bind with high affinity to the 3’-OH placed at the end of the nascent leading strand at the fork. When both the 3’-OH and SSB are present, the maximum effect is observed. This ensures that PriA will only load onto the correct fork, in the right orientation, thereby ensuring that replication restart is directed to only the template lagging strand." @default.
- W3108968498 created "2020-12-07" @default.
- W3108968498 creator A5002684349 @default.
- W3108968498 creator A5036019614 @default.
- W3108968498 date "2020-11-23" @default.
- W3108968498 modified "2023-09-28" @default.
- W3108968498 title "SSB facilitates fork substrate discrimination by PriA" @default.
- W3108968498 cites W1518548089 @default.
- W3108968498 cites W1522284441 @default.
- W3108968498 cites W1525204026 @default.
- W3108968498 cites W1544482700 @default.
- W3108968498 cites W1559132509 @default.
- W3108968498 cites W1560669282 @default.
- W3108968498 cites W1570361288 @default.
- W3108968498 cites W1583600685 @default.
- W3108968498 cites W1626070385 @default.
- W3108968498 cites W1965780315 @default.
- W3108968498 cites W1968035605 @default.
- W3108968498 cites W1969725499 @default.
- W3108968498 cites W1974380099 @default.
- W3108968498 cites W1980299999 @default.
- W3108968498 cites W1985188411 @default.
- W3108968498 cites W1986104253 @default.
- W3108968498 cites W1987314616 @default.
- W3108968498 cites W1995761114 @default.
- W3108968498 cites W1997139387 @default.
- W3108968498 cites W1998772598 @default.
- W3108968498 cites W2004392681 @default.
- W3108968498 cites W2005596792 @default.
- W3108968498 cites W2005699935 @default.
- W3108968498 cites W2005986251 @default.
- W3108968498 cites W2008250450 @default.
- W3108968498 cites W2013291882 @default.
- W3108968498 cites W2013735908 @default.
- W3108968498 cites W2015366670 @default.
- W3108968498 cites W2021063616 @default.
- W3108968498 cites W2025475717 @default.
- W3108968498 cites W2026635338 @default.
- W3108968498 cites W2050150719 @default.
- W3108968498 cites W2051530487 @default.
- W3108968498 cites W2051555014 @default.
- W3108968498 cites W2056375209 @default.
- W3108968498 cites W2061713612 @default.
- W3108968498 cites W2065976083 @default.
- W3108968498 cites W2066427226 @default.
- W3108968498 cites W2066638568 @default.
- W3108968498 cites W2069361954 @default.
- W3108968498 cites W2070872901 @default.
- W3108968498 cites W2080857411 @default.
- W3108968498 cites W2084158547 @default.
- W3108968498 cites W2089298763 @default.
- W3108968498 cites W2091529967 @default.
- W3108968498 cites W2093085132 @default.
- W3108968498 cites W2095833603 @default.
- W3108968498 cites W2099764109 @default.
- W3108968498 cites W2100489858 @default.
- W3108968498 cites W2101330556 @default.
- W3108968498 cites W2105444563 @default.
- W3108968498 cites W2105791961 @default.
- W3108968498 cites W2127979283 @default.
- W3108968498 cites W2134225956 @default.
- W3108968498 cites W2147220402 @default.
- W3108968498 cites W2154773952 @default.
- W3108968498 cites W2160336344 @default.
- W3108968498 cites W2163548075 @default.
- W3108968498 cites W2167342214 @default.
- W3108968498 cites W2170192355 @default.
- W3108968498 cites W2191824721 @default.
- W3108968498 cites W2236039528 @default.
- W3108968498 cites W2306853147 @default.
- W3108968498 cites W2317960638 @default.
- W3108968498 cites W2410925476 @default.
- W3108968498 cites W2574055941 @default.
- W3108968498 cites W2754057704 @default.
- W3108968498 cites W2891905845 @default.
- W3108968498 cites W2921031054 @default.
- W3108968498 cites W2982280598 @default.
- W3108968498 cites W3012258601 @default.
- W3108968498 cites W3013595405 @default.
- W3108968498 cites W3014889156 @default.
- W3108968498 cites W4246968887 @default.
- W3108968498 doi "https://doi.org/10.1101/2020.11.23.394411" @default.
- W3108968498 hasPublicationYear "2020" @default.
- W3108968498 type Work @default.
- W3108968498 sameAs 3108968498 @default.
- W3108968498 citedByCount "0" @default.
- W3108968498 crossrefType "posted-content" @default.
- W3108968498 hasAuthorship W3108968498A5002684349 @default.
- W3108968498 hasAuthorship W3108968498A5036019614 @default.
- W3108968498 hasBestOaLocation W31089684981 @default.
- W3108968498 hasConcept C104317684 @default.
- W3108968498 hasConcept C111919701 @default.
- W3108968498 hasConcept C12554922 @default.
- W3108968498 hasConcept C161223559 @default.
- W3108968498 hasConcept C185592680 @default.
- W3108968498 hasConcept C40523978 @default.
- W3108968498 hasConcept C41008148 @default.
- W3108968498 hasConcept C54355233 @default.
- W3108968498 hasConcept C552990157 @default.
- W3108968498 hasConcept C67705224 @default.