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- W310910588 abstract "HSP 90, a member of heat shock proteins (HSP) plays key role in moderating the heat stress of the animal. Zebu cattle (Bos indicus) possessing this gene display better heat resistance. This study details the single stranded conformational polymorphism (SSCP) pattern of exon 8, 9, and 10 of HSP90AA1 gene in Deoni cattle, a premier dual purpose (dairy and draught) breed in peninsular India, which was not investigated earlier. The study was conducted on Deoni cows maintained at the institute farm at Bangalore. The genomic DNA was isolated by Miller’s High Salt method, and the purity was confirmed on the basis of OD ratio (260nm/280nm). Primers, specific to coding sequence of HSP90AA1 gene were designed using Primer 3 software and amplified by polymerase chain reaction (PCR). PCR products were subjected to Single-Strand Conformation Polymorphism (SSCP) analysis, to determine the genetic variants. Exon 8 revealed three unique SSCP patterns, labeled as pattern I, pattern II and pattern III, which showed one, two, and six distinct DNA bands, in the genotypic frequency of 0.250, 0.639, and 0.111, respectively. Exon 9 revealed two unique SSCP patterns, labeled as pattern I and pattern II, which showed two and three distinct DNA bands, in the genotypic frequency of 0.153 and 0.847, respectively. Exon 10 revealed two unique SSCP patterns, labeled as pattern I and pattern II, which showed two and three distinct DNA bands, in the genotypic frequency of 0.236 and 0.764, respectively. This study concludes that SSCP polymorphism with in exon 8, exon 9, and exon 10 of HSP90AA1 gene in Deoni cattle, can be further exercised to explore their linkage with economically important traits in this breed." @default.
- W310910588 created "2016-06-24" @default.
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- W310910588 date "2014-01-01" @default.
- W310910588 modified "2023-09-23" @default.
- W310910588 title "Genetic polymorphisms within exon 8, 9, and 10 of heat shock protein (HSP) 90AA1 in Deoni cattle." @default.
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